Undergraduate Theses
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Item Mud crab (Scylla serrata) shells for the removal of humic acids from Santa Barbara well waterAsdillo, Reina Jean A. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2010-04)The chemical contamination of drinking water from a wide range of contaminants such as humic acids can potentially lead to health-related in living organisms, especially humans. In rural areas of the Philippines, the use of low cost methods to remove water pollutants is, therefore, of great interest. In this study, osterized mud crab (Scylla serrata) shells or exoskeletons from cooked crab wastes were used to remove humic acids from the Santa Barbara well water. Adsorption experiments were done to evaluate its capacity to remove humic acids from the well water. Adsorption Isotherms and Fourier Transform Infrared Spectroscopy were employed to study the characteristics of the adsorption of humic acids onto the OSSE (osterized Scylla serrata exoskeletons). Adsorption experiments showed that at an average 1.0010 g of OSSE and after six hours of agitation, an average 74.95% of humic substances were removed from 50 mL of Montinola, Santa Barbara well water sample. Isotherm experiments showed that the experimental data can be fitted in both Langmuir and Freundlich models with low K values of 0.0235 L/mg and 0.4450 (mg/g)(L/mg), respectively. Based on the results of the above experiments, OSSE is a good adsorbent for humic acids in well waters (although more research need to be done on the removal of the substances remaining in the water after OSSE treatment) and the U-OSSE (used osterized Scylla serrata exoskeletons) can possibly be recycled.Item Comparison of protein quality of three seaweed species (Sargasum polycystum, Sargasusum oligocystum and Ulva pertusa) from Panay and Guimaras Island, Philippines for potential use in milkfish feedArnaiz, Regina Marielle T. (Division of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2016-06)Aquafeed production is the largest expenditure in intensive aquaculture operations, contributing to about 40-60% of total costs (Yildrim et al., 2009; Akiyama et al., 1992 cited in Ali et al., 2009). This is mostly due to the ingredients being used, particularly the protein source fishmeal (Millamena, 2002). The variable and finite supple of fishmeal, however, result in high costs, prompting the search for cheaper alternative ingredients as a priority in cost reduction practices regarding the production of aquafeeds (Attala and Mikhail, 2008). Seaweeds have known nutritional values, and are naturally abundant in Philippine waters, thus their potential could be tapped to reduce costs in aquaculture and could therefore maintain the sustainability of the industry (Shields and Lupatsch, 2012; Trono, 1999). In this study, the protein quality of two brown seaweeds (Sargassum polycystum and Sargassum oligocystum) and one green seaweed (Ulva pertusa) was evaluated by determining their crude protein content, inhibitory activities against trypsin and chymotrypsin, and in vitro protein digestibility. Among the three species, the green seaweed U. pertusa has the highest crude protein content at 23.43%. The obtained crude protein content was 8.28% for S. polycystum and 9.22% for S. oligocystum. Trypsin inhibition of the seaweeds ranged from 54-57%, and chymotrypsin inhibition was from 57-64%. Total phenolic content (TPC) was highest in S. polycystum, and lowest in S. oligocystum. In vitro protein digestibility action of milkfish gut enzyme was highest in S. oligocystum (92.30 ± 2.80), followed by the observed digestibility in U. pertusa (75.01 ± 2.14), and lowest in S. polycystum (64.99 ± 8.11). Determination of Pearson's correlation (r) showed a negative correlation (r=-0.99, p=0.08) existing between the TPC and %RPD values. Based on the results, among the threes species, U. pertusa with 23.43% crude protein content and a %RPD of 75.01% has a potential for partial inclusion in aquafeeds to reduce costs.Item Treatment of Vibrio harveyi-infected Penaeus monodon using Alibutbut (Tabernaemontana pandacaqui Poir.) plant powderBacaling, Marivic T. (Division of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Visayas, 2005-04)The cytotoxicity test of crude methanolic leaf extract using Brine Shrimp Lethality Assay revealed that Alibutbut (Tabernaemontana pandacaqui Poir.) is safe to use in treating vibrio-infected penaeid shrimps. The effectiveness of the alibutbut powder against vibriosis was examined through the determination of the post-larvae 20 (PL 20) penaeid shrimp mortality and the water microbial assay after 48 hours. Percent penaied shrimp mortality (0-6.67%) and microbial count (2.91 x 10^4- 3.26 x 104 cfu/mL) of the plant treatments were significantly lower compared to the negative control (no treatment) that exhibited 50% penaeid shrimp mortality and a microbial count greater than 6.00 x 10^4 cfu/mL. The results of the experiment showed that Alibutbut plant powder is indeed effective in controlling Vibrio harveyi bacteria.Item Determination of heavy metal (Cu, Cd, and Pb) concentration in musclemeat of fish from different areas in Batan Bay, AklanBagarinao, Cathrina F. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)Food safety is of utmost importance but it is usually compromised by the presence of pollutants in the environment such as heavy metals. This study was conducted to investigate heavy metals concentrations in fish musclemeat collected from different areas of Batan Bay, Aklan. A total of 22 fish species were analyzed for Cu, Cd and Pb using Flame Atomic Absorbance Spectroscopy. Pb concentration in the musclemeat of fish ranged from 0.0292 ± 0.2070- 2.09 ±1.15 mg/kg wet weight and was found to be highest in Mugil cephalus (flathead grey mullet), while Cd concentration ranged from 0.0075 ± 0.0016-0.157 ± 0.209 mg/kg wet weight with highest value in Megalops cyprinoides (Indo-pacific tarpon), and Cu concentration ranged from 0.0605 ± 0.0667-2.76 ± 0.05 mg/kg wet weight, with highest concentration in Leiognathus equulus (common ponyfish). Among the metals, only Pb concentration in flathead grey mullet exceeded the maximum permissible concentration in food as indicated by the Joint FAO/WHO Codex Alimentarius (1984). Assessment of Noncarcinogenic Hazard Quotient (NHQ) gave NHQ values less than 1, indicating that the risk of developing adverse effects due to fish intake is low.Item Effects of high and low protein diets on the gut evacuation rate of Tilapia niloticaAndrada, Bryan Andrew S. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1999-03)Gut evacuation rate of young Nile tilapia, Tilapia nilotica fed commercial pelleted diets containing high or low protein diet were determined in the laboratory. The decline in gut fullness of the fish with time was examined at low and high protein diets. Two mathematical models were used to express the decline in gut fullness of the fish with time. Fish fed the low protein diet followed more closely the linear model while those fed the high protein diet followed the logarithmic model. The level of protein resulted in different gut evacuation rates (GER), expressed as coefficients b of the gut fullnesstime curve. GER values were higher in either linear or logarithmic models for fish fed low protein diet than for those fed high protein diet.Item Diel feeding patterns of angelwing clam Pholas (Monothyra) orientales (Gmelin, 1970)Alojepan, Marbe Joy L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1999-04)Filtration rate of Pholas orientalis measured using the indirect suspension deletion method and monitored by direct algal cell counts. Phytoplankton Isochrysis galbana was used as food at an algal cell concentration of 2.5 x 103 cells ml ”'. Biological rhythms have been demonstrated in all animals and plants except bacteria. They control rhythmic patterns in behavior and physiology of a living organism. Biological rhythms, or oscillations, have a “period” that is measured from one arbitrary point or phase of the rhythm to the next occurrence of that phase. They are best demonstrated in a 24 h cycle or circadian cycle. There is a widely varying feeding activity in the 24h cycle of P. orientalis observed at a constant temperature of 27$C and hydrogen ion concentration of 35%o Like any other bivalves, P. orientalis has defined feeding periods equivalent to food uptake at 2400h-0300h. Such observation could be supported by a previous study by Corda stating that optimal feeding was obtained at total darkness. Periods of digestion occur shortly after the periods of feeding. P. orientalis when actively feeding were observed to have extended siphons, larger valve openings, and an inclination of 45. One primary external stimulus that affected filtration rate was the amount of light present. Biorhythms provide temporal information, such as the time of day or season of the year, which facilitates adaptation of the organism to internal or external environments.Item Effect of Physico-chemical parameters of rearing waters on survival rates of larvae and post larvae of Penaeus monodon in two hatchery systemsAlindong, Ray Lauron (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1995-03)Physico-chemical parameters such as temperature, pH, salinity, and D.O. of the small-scale and large-scale hatchery system were monitored daily for a duration of two months. The relationship between the physico-chemical parameters and the survival of the different larval and post-larval stages between the two hatchery systems was then determined. Results showed that the physico-chemical parameters obtained from the small-scale hatchery system were favorable to the survival of the larval stages of the prawn. However, the physico—chemical parameters obtained from the large-scale hatchery system were more suitable for the growth of the post-larval stages.Item Heavy metal concentrations in sediments from Batan Bay and its tributariesAndrada, Mary Royce D. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)The concentration of three heavy metals (Cd, Pb, Cu) in sediments from Batan Bay and its tributaries were determined in order to assess the degree of contamination of sediments in the said area. The samples were digested following the Method 3050B of US Environmental Protection Program (EPA, 1996). The heavy metal concentrations were determined using Flame Atomic Absorption Spectrometer (FAAS). Results show that the heavy metal concentrations ranged from n.d. - 2.57 mg/kg dry weight (dw) for Cd; 16.44-143.02 mg/kg dw for Cu; and 3.18-28.53 mg/kg dw for Pb. Sediment sample collected from BTN #10 has the highest Cd concentration while sediment sample collected from BTN 052 which is a rice field in Batan, Aklan registered the highest Pb and Cu concentrations. Three sediment quality guidelines (National Oceanic and Atmospheric Administration, Canadian Sediment Quality Guideline and Hong Kong Sediment Quality Guideline) were employed to assess the metal contamination in sediments. The concentrations of Cd and Pb in all sites did not exceed the maximum permissible limits set by the three guidelines. As for Cu, BTN 052 exceeded the Upper Chemical Exceedance Level set by Hong Kong SQG. Correlation analysis showed that there is a significant correlation (p <0.01) between Pb and Cu indicating possible similar source input. Special attention must be given to BTN 052 since the levels of the studied heavy metals are high in this area. The results can be used as basis for the pollution level of sediments from Batan Bay and its tributaries. Special consideration should be given to BTN 052 since all of the test metals are significantly high in this area. It is also suggested to in identify the primary sources of metal contamination in this area.Item Effects of freezing and thawing on some agar parameters of Eucheuma spinosum and Gracilaria confervoidesAbelende, Ma. Orfa R. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1995-05)Agar was extracted from two agar-containing red algae species, namely Gracilaria confervoides and Eucheuma spinosum. Qualitative assessment was made on the agar extracts, which included percentage yield, gel strength, syneresis, and hysteresis range. The quality of agar extracts derived from this study was compared to commercial agar-agar. The. effects of each parameters, repeated freezing and thawing on agar were also investigated. Qualitative test showed that percentage yield from Gracilaria was higher than Eucheuma. Gel strength and hysteresis range of agar from commercial sources and Gracilaria were higher compared to Eucheuma. On the other hand, syneresis range was highest in Eucheuma agar. Repeated freezing and thawing lowered percentage yield and syneresis but increased gel strength and hysteresis of agar extracts from agar sources used.Item Comparison between commercial pellets and golden apple snail pellets as food for Tilapia nilotica fingerlingsArboleda, Daphne A. (Dision of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1997-04)The viability of Golden apple snail as food for Tilapia nilotica was determined by comparing it with commercial pellets. The experiment was conducted on February 3, 1997. The two treatments had three replicates each with a stocking density of 15 fingerlings each. Comparison of growth curves showed that fingerlings fed with commercial pellets grew significantly (P< 0.01) faster than those fed with golden apple snail pellets. The computed FCR was also greater for commercial pellets. The results suggest that golden apple snail alone does not promote efficient growth in Tilapia nilotica fingerlings. Thus it is recommended that golden apple snail be combined with other food ingredients to enable its efficient utilization as a protein source.
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