Undergraduate Theses

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A comparison on the effect of light on melanophore differentiation in the larval stage of frog, Hyla sp.
Araneta, Merrie Celine E. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1996-03)
Three set-ups were exposed to different experimental conditions namely: set-up A. under prolonged light: set-up B. under prolonged darkness: set-up C. under normal condition. Observation was done under the dissecting microscope from the first appearance of melanophores until the migration was stabilized. The pattern of differentiation was observed to be the same in all set-ups. only the distribution and the color intensity differred. B was the darkest and C was the lightest. It only showed that light could not, alter the pattern of melanophore differentiation, only the distribution.
A survey of the catch composition of fyke nets in the Iloilo river
Andrada, Kristina Abelaine D. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2000-04)
A survey of the catch composition of fyke nets in the Iloilo River Estuary during the months of August 1999 and February 2000 show that catches are dominated by Metapenaens crisis and (ilossygobius sp. The 18 species caught are : 9 fish species, 5 shrimp species, and 4 crab species. The index of similarity 0.3 between the monthly samples is low which means that there is minimal degree of similarity between the species composition of the catches. There is no significant difference between the total catch volumes per day for the months of August 1999 and February 2000 which means that the seasons do not affect the volume of the catch.
Academic performance of UPV Iloilo High School students admitted under the democratized admission scheme
Apalacio, Reymund D. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1993-04)
As its contribution to the enhancement of the social welfare of people, especially the poor, in a poor developing country like the Philippines, a government university like UP has embarked on a policy democratized education which includes the scheme of democratized admission at the UPV Iloilo High School. This paper undertook action research, which is the applications of tools and methods of social science to immediate, practical problems, to determine the academic performance of economically poor students admitted under the new, democratized admission scheme of the UPV Iloilo High School. It was found out that generally they perform as well as their better-off counterparts under the old scheme (with tuition fees).
Adaptive capacity of the local government to the impacts of climate change: comparison between Guimbal and Tigbauan
Andal, Jane Desiree F. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2011)
The paper examined the factors affecting the adaptive capacity of the local government in terms of responsiveness and preparedness to deal with the impacts of climate change. This is focused on three factors namely, organizational learning, collaboration and socio-economic status. The study is conducted by comparing the municipalities of Guimbal and Tigbauan in terms of their organizational learning, collaboration and socio-economic status as well as their responsiveness and preparedness in dealing with typhoons, flooding, and drought as the major impacts considered in this study. The results show that organizational learning enhances the preparedness of the municipality to deal with the impacts while collaboration and socio-economic status enhance both responsiveness and preparedness to deal with the impacts of climate change.
Antioxidant and antibacterial activities of bika (Ampelocissus martini panch) seed oil
Angot, Rose Imee Lyn N. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2016-06)
Ampelocissus martini Planch, locally known as Bika, is a native tropical plant generally found in Southeast Asia. It is abundant in Panay Island, particularly in the provinces of Antique and Iloilo. Despite that the fruit is being consumed as food, used as medicine, and made into wine, very few scientific studies pertaining to Bika grape exist. Hence, this study focused on its antioxidant and antibacterial activities. The antioxidant activities were carried out through the % DPPH radical scavenging activity and reducing power method. Results show that the Bika seed oil has antioxidant activity. Its IC50 using DPPH assay is 23.63 ppm. Moreover, it also showed a significant antioxidant activity based on the results from the reducing power assay. Both results demonstrated that Bika grape seed oil have antioxidant activity. On the other hand, the mean zones of inhibition using disk diffusion assay was used to assess the antibacterial activity of Bika seed oil against Escherichia coh and Staphylococcus aureus. Only the antibiotic Gentamicin showed active antibacterial property, whereas the Bika seed oil showed little zone of inhibition, only goes to show that it has an inactive antibacterial property.
Assessment of induced stress as schemes for selectively removing fish with opercular deformities in hatchery produced milkfish (Chanos chanos) seeds
Cordero, Christian P. (College of Fisheries and Ocean Sciences, University of the Philippines Visayas, 2015-06)
The effects of different types of induced stress were tested as schemes for the elimination of fish with opercular deformities in hatchery produced milkfish seeds. Salinity and pH were used as the main stressors for milkfish larvae of different ages. Induced salinity stress was initially tested on larvae at 15 days post-hatch (dph) by directly transferring the larvae from its rearing water salinity of 32 ppt to salinities of 10 ppt, 25 ppt, 35 ppt and 45 ppt. Almost all of the larvae subjected to salinity stress dies, hence, it was deemed necessary to examine the influence of handling stress and to determine the age of larvae that could tolerate handling. Larvae of different ages (from 10 dph to 22 dph) were thus subjected to handling stress by collecting them from the mass rearing tank and transferring them to 1000 ml beakers where survival was monitored. Results showed that milkfish larvae were able to withstand handling stress beginning 21 dph. Considering that milkfish fry (21 dph) are already known to tolerate direct transfers to various salinities, the next experiments were focused on pH stress. The suitability reduced or increased pH as stressors were initially tested by directly transferring larvae from normal seawater (pH 7.50) to acidic water (pH 6.5-7.0) and basic seawater (pH 8.0-8.5). Results revealed several advantages of reducing pH levels over increasing pH levels, which included the comparably higher larval survival, the lesser amount of chemicals required to change pH levels and the corresponding cost of these chemicals. A range finding test was then conducted to determine the optimum pH that could selectively remove fry with opercular deformities by directly transferring fry reared in normal seawater (pH 7.50) to seawater with reduced pH: Trt 1-pH 6.5; Trt 2-pH 6.6; Trt 3-pH 6.7; and Trt 4-6.8. Larval mortalities were recorded after 1 h, then 25% water change was done for all treatments which were monitored further for mortalities after 2h, 3h, 5h, and 24h. The surviving larvae from all the treatments were reared further for 18 days (until 40 dph) in order to monitor opercular deformities which can observed only when the larvae have metamorphosed into juveniles. Results showed that a sudden drop in pH of normal seawater to pH 6.5-6.7 selectively removed all the fish with opercular deformities in hatchery produced milkfish seeds; at such pH range, more than 90% survival of milkfish fry was obtained with this batch of larvae and all the surviving fish had no opercular deformities.
Bacteriological analysis of seawater from San Salvador to Belen bay areas in Banate Bay, Banate, Iloilo
Abancio, Celyn Ann L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines in Visayas, 2000-04)
This study was conducted at Banate bay, Banate, Iloilo from a point from Belen to San Salvador. Two samplings were taken one week apart and was analyzed for the Most Probable Number (MPN) per ml coliform count The Multiple fermentation tube technique showed that in Poblacion approximately 100 meters from the shore had the lowest MPN values for both samplings which was >3 MPN per ml. The highest count of greater than or equal to 2400 MPN per ml was obtained in sites Belen-10, Belen-50, and Bularan-10 meters away from the shore. As the distance from the shore increases, the MPN values usually decreases, showing that the area near the shore would exhibit high coliform counts than the are further away from the shore. The tide and the weather may have affected the coliform counts because of the differences of MPN values.
Bioactivity-guided screening of selected plants from UPV Miag-ao campus
Arceo, Ma. Ofelia H. (Division of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Visayas, 1998-05)
Twelve selected ethnomedically-used plants were screened for alkaloids, saponins, and flavonoids. Nine out of twelve plants contained at most two of the phytochemicals tested, and three showed the absence of these phytochemicals. Methanolic extracts of three selected plants (Stachy/arpheta /amaicensis, Hyptis suaveo/ens, Semecarpus philippinensis) were examined for antibacterial activity against Staphylococcus aureus. Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. All extracts were found to be active at least against two of the microorganisms tested. The extract of Siachytarphe/a jamaicensis was found to be active against three out of four microorganisms and found to be the most bioactive. Stachyiarpheta jamaicensis was subjected to bioactivity-guided fractionation using solvents of increasing polarity - hexane, ethyl acetate, and n-butanol. The hexane extract was considered as the most bioactive fraction. Its chromatogram contained seven spots in which four turned blue violet after spraying with vanillin-H2SO4. The color change exhibited suggests the possible presence of saponins.
Chromatographic analysis and antibacterial assay of Avicennia officinalis bark extracts
Janagap, Steve P. (Division of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Visayas, 2003-04)
In this study, the antibacterial activity and the chemical constituents of Avicennia officinalis bark were determined. The antibacterial assay showed that the dichloromethane (DCM) fraction at 1000 ug/mL was most effective against the tested bacteria especially to Staphylococcus aureus. It had a microbial index of 0.97 while the positive control, amoxicillin had 1.00. The aqueous fraction did not show antibacterial activity to the organisms tested. A number of chemical constituents were found to be present in the different fractions obtained from Avicennia officinalis bark: alkaloids, cardiac glycosides, flavonoids, phenolic compounds, saponins and terpenes. These constituents were determined using thin layer chromatography. The DCM fraction which showed the highest bioactivity, had the most number of components while the aqueous fraction had the least number of components. The DCM fraction was subjected to two-dimensional thin layer chromatography. Nine spots were observed with the first solvent system (ethyl acetate-DCM; 5:3) while eleven spots were observed with the second solvent system (ethyl acetate-n-hexane-DCM; 2:1:1).
Community structure of a ten-year old naturally, regenerating mangrove stand in Baguingin, Tigbauan, Iloilo
Animas, Ruphil May J. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1999-04)
The structure of the mangrove community in Baguingin, Tigbauan was studied using the transect line plot method with seven plots established. Community structure is described in terms of the following parameters: basal area, stems per hectare, relative density, relative frequency, relative dominance, and importance value of the species and the Shannon Index of Diversity. Variations in these parameters are correlated with certain physico-chemical factors-- air and water temperature, soil pH, water salinity, land elevation- and the degree of human influence. Three true mangrove species were found in the area: Excoecaria agallocha, Avicennia marina, and Avicennia alba. A. marina was found to have the highest relative density of 70.8%. relative dominance of 52.35% and importance value of 169.82. A. marina and A. alba have equal relative frequency of 46.67%. Seedlings and saplings of A. marinadominate the area, indicating that the community is a young and regenerating stand. Basal area significantly increased from sea to land ( 53.68 cm2 to 431.66 cm2) and from creek to land ( 8.83 cm2 to 366.54 cm2). Shannon Index of Diversity gave a result of 0. The very low diversity index means that there is uneven distribution of individual trees among the species, and only a few kinds of species were present and are centered around only two genera, Avicennia species and Excoecaria agallocha.
Determination of the anthocyanin content in Hibiscus sabdariffa L. (Roselle) calyses from fresh to spray-dried form
Arganioza, Hannah Mae C. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2015-06)
Hibiscus sabdariffa L. is an agricultural herb with calyces as the commercially important part this plant. The calyces is a good source of anthocyanin (pigments) with several potential applications in the food, pharmaceutical and cosmetic industries. These pigments are used worldwide in the production of drinks and as a source of natural food coloring. However, these pigments are very unstable due to processing and storage conditions. Thus, this present study investigated the amount of anthocyanin as it is processed into a commercial product in the form of spray-dried powder. The Roselle plant samples were obtained from Herbanext Laboratories farm. The fresh extract has 493.45 mg/L total monomeric anthocyanin. Compared to the fresh extract, concentrated aqueous spray-dried extract (261.06 mg/L) contains lower anthocyanin content, however, this is significantly higher than aqueous concentrate (124.41 mg/L). Both the aqueous extract and ethanolic extract contains the smallest amount of anthocyanin for each sets. Spray drying techniques increases the amount of anthocyanin which helps to prevent further degradation of the compound due to the influences of the storage environment and processing conditions.
Effect of varying levels of cotton (Gossypium sp.) seed meal on the spermatogenesis of mice (Mus musculus)
Abonador, Luena A. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1992-04)
Cottonseeds procured from Western Colton Corporation, Oton, Iloilo were dehulled, cleaned by hand, and ground using a mortar and pestle. Ground cottonseed meal (CSM) was incorporated in varying amounts into the basal diet so that diets contained varying levels (0,10,20,30 g) of CSM per 100 g formulated diet. These were then pelletized at the Nutrition and Feed Division, Aquaculture Department (AQD), Southeast Asian Fisheries Development Center (SEAFDEC), Tigbauan, Iloilo. A total of thirty-two 20-day-old male mice (16 white and 16 black) were randomly distributed to four dietary treatments, each with four replicates. After a 5-day acclimatization period, during which the mice were fed with the basal diet (0% CSM), the four experimental diets were given to the randomly selected experimental animals for another 35 days. The mice were subsequently sacrificed, and histological examination of the testes was conducted. Differential count of sperms was done to evaluate the effects of varying levels of CSM on the spermatogenesis of mice. The data obtained were analyzed statistically using analysis of variance followed by Duncan’s Multiple Range Test. There was no significant difference in the sperm counts of the two strains of mice. However, significant differences (P<0.01) were noted in sperm counts of mice at various levels of CSM in the diet. Likewise, significant differences (P<0.01) were observed between 0% and 20 or 30%; 10% and 20 or 30%; 20% and 30% CSM in the diet. No significant difference was noted between sperm counts of mice fed with 0% and 10% CSM levels. Slight degeneration of the seminiferous tubules and predominance of immature stages (spermatogonia) were observed in the testes of mice fed with diet containing 30% CSM.
Fears of Doña Montserrat Lopez Memorial High School students, Silay City, Negros Occidental
Arcenas, Maricar D. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-04)
The study focuses on the different types of fears of Doña Montserrat Lopez Memorial High School students in Silay City, Negros Occidental. The aim of the study is to identify the different types of fears of Doña Montserrat Lopez Memorial High School students? to determine the most intensely Feared abject and the least intensely feared object on every fear category. For every gender, per year levels and to determine whether there is gender difference on the reported intensity for each type of fear, per year level. There were 245 respondents, 85 of which were Freshmen, 51 were Sophomores, 64 were Juniors, and 45 were Seniors. The respondents were selected through stratified random sampling using proportional allocation. The design that was used in this study is the survey. A questionnaire and a checklist were used to identity the different types of fear of the respondents and the trend of its intensity. The trend of the intensity on the different types of fear were measured using the formula of the average score and the T test formula. The findings showed that Doña Montserrat Lopez Memorial High School students have fear of supernaturals, animals, nature natural calamities; man-made calamities; means oF transportation; places; fear of certain criminals; and fear of crimes. The intensity report showed that there are similarities and differences on the reported most intensely feared objects and least intensely feared object on the different fear categories, per year level. Furthermore, the result of the study also showed that there is no gender difference on the intensity report on most feared objects per year level.
Fecundity and egg quality in two species of siganids (Siganus canaliculatus and Siganus fuscescens) from Lanuza Bay, Surigao del Sur, Philippines
Anore, Gaguis Anito II T. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2019-06)
Siganus canaliculatus and Siganus fuscescens are two of the important yet highly exploited siganid aquatic resources in the Philippines. Only few studies for both species, especially for S. fuscescens, are available in the Philippine setting regarding its fecundity and egg quality. This study was conducted to provide information on the fecundity and egg quality of S. canaliculatus and S. fuscescens from Lanuza Bay, Surigao del Sur, Philippines. Fecundity was determined through gravimetric method in individual gonads. Fecundity range was determined to be 54,856 — 279,957 individual oocytes for S. canaliculatus and 7,397 — 563,722 for S. fuscescens. Fecundities of both species exhibited a weak to strong positive correlation to size, with higher correlation coefficient to standard length for S. canaliculatus and body weight for S. fuscescens. On the other hand, egg quality was determined through mean oocyte diameter, with diameters ranging from 176 — 426 ųm for S. canaliculatus and 171 — 500 pm for S. fuscescens. Mean oocyte diameters of both species exhibited weak positive correlation to size, but with a higher correlation coefficient on standard length. Regulating the capture of smaller-sized fishes and allowing them to grow would promote the increase in fecundity and egg quality of the species.
Gahom sa binalaybay: Mayamor's poetry as vehicle of power in the resistance movement of Panay
Arceño, Angeliza T. (Division of Humanities, College of Arts and Sciences, University of the Philippines Visayas, 2016-12)
This study analyzes Mayamor’s poetry as a vehicle of power in the resistance movement of Panay. Using New Historicism and textual analysis, this study identifies the cultural status quo in Mayamor’s poetry as Feudal and describes its manifestations and his mechanisms to counter it by waging a National Democratic revolution through a protracted people’s war and cultural revolution. Mayamor’s poems serve as a vehicle of power in the resistance movement of Panay by using poetry as a tool to teach people about the situation in the countryside of Central Panay, for exposing the cruelty of the state and the ruling class, by being a testimony to the plight of the people, and by inviting them to be allies and join the struggle in the countryside.
Geochemical association of Cr, Cu, Ni, and Pb in the sediments of Iloilo River estuary
Arban, John Paul B. (Division of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Visayas, 2005-04)
The geochemical association of Cr, Cu, Ni, and Pb in the sediments of Iloilo River Estuary has been studied using a modified Tessier’s sequential extraction procedure. The results obtained show that most of the metals studied are mostly associated in the residual and organic matter fractions of the sediment samples (Cr: 66.26% - 74.36% in the residual,15.43% - 24.08% in the Fe-Mn oxide fraction, 8.14% - 12.71% in organic matter fraction, and 0.000% - 2.996% in the exchangeable/carbonate fraction; Cu: 39.30% - 46.73% in the residual, 12.00% - 52.18% in the organic fraction, 3.227% - 11.41% in the Fe-Mn fraction, and 0.000% - 17.43% in the exchangeable/carbonate fractions; Ni: 42.75% - 57.02% in the organic matter fraction, 24.93% - 39.33% in the residual, 5.814% - 14.15% in the Fe-Mn fraction, and 5.504% - 13.12% exchangeable/carbonate fraction; and Pb in sample AAA-21: 48.02% in the residual, 38.55% in the organic matter fraction, and 7.33% in the Fe-Mn fraction, and 6.078% in the exchangeable/carbonate fraction). The analysis of the total metal concentrations of the sediment samples taken from Iloilo River are found to be above the reported concentration of uncontaminated sediment except for Pb in sediment sample AAA-21 (Chromium: 102.1 ug/g - 150.5 pg/g; Copper: 62.56 pg/g - 111.7 pg/g; Nickel: 53.40 pg/g - 75.44 pg/g; and Lead in sample AAA-21: 22.21 pg/g). The study shows that about 50% of the total metal concentration in the sediment samples are bioavailable to the aquatic organisms.
Growth of the telescope snail, telescopium telescopium (Linne) in brackishwater ponds
Apistar, Jobert H. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines, 1998-04)
Samples of T. telescopium were collected every month, from April to October 1997 for 6 months, in brackishwater ponds. Allometric relationships were defined by comparing shell length with shell width, as well as various body weight proportions including total weight with shell, total volume with shell, visceral weight and visceral volume. A total of 5 cohorts with growth rates ranging from 0.45 - 0.67 mm-day- were observed using the Bhattacharya analysis. A high mean growth rate of 0.56 mm-day- (sd = 0.07, n= 5) was estimated by joining the modes of the cohorts between successive sampling months. Low inverse correlations were observed between mean shell length and various environmental parameters (i.e. salinity, air temperature, water temperature and organic matter content) possibly explained by the species' environmental adaptations. On the other hand significant correlation was observed between mean shell length and density, indicating the possible role of overcrowding in these habitats.
Health risks associated with bacteriological quality of bathing waters in Villa Beach, Brgy. Sto. Niño Sur, Arevalo, Iloilo City
Abad, Sue Ellen T.; Catinan, Mary Joy A.; Hautea, Therese Angelie T.; Mabang, Moctar M.; Narida, Leah Joy S.; Panggat, Kevin Matthew B.; Turija, Maisie Mae L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2004-04)
ABSTRACT A special problem submitted to Division of Biological Sciences, College of Arts and Sciences, University of the Philippines in the Visayas, Miagao, Iloilo. The study investigated the bacteriological quality, using E. coli, Salmonella spp., and culturable Vibrios as bacterial indicators, of four selected bathing waters in Villa Beach, Brgy. Sto. Nino Sur, Arevalo, Iloilo City, as well as the incidence of symptom occurrence associated with these microbes among 33 respondents. This was to determine health risks associated with bacteriological quality of bathing waters in Villa Beach. Conventional and modified laboratory procedures were used in the detection of E. coli, Salmonella spp., and culturable Vibrios, while pre-structured interview schedule was utilized in the epidemiological survey. p-value was used as statistical tool to measure association. All four water samples tested positive for E. coli and culturable Vibrios. Salmonella spp. was negative in all of the water samples. The MPN for E. coli in water sample C was 460 per 100 ml water sample, while in water samples A, B and D it was 240. Obtained MPN were above the DENR MPN standard for fecal coliform for Class SB, which is only 200. There were 52 individual participated in the study but only 33 (63 %) were able to complete the follow up interview done seven days after the actual date of survey. 18 (55 %) of the respondents were females. The mean (SD) age was 19.3 (14.5) years with an age range of 3-56 years. 11 (34 %) respondents came from sampling site C and 32 (97 %) were visitor-swimmers. Only 13 (39 %) of the respondents reported to have experienced symptoms during the follow up, and the most common symptoms reported by 10 (77 %) of the symptomatic respondents were upper respiratory tract symptoms such as colds, cough and sore throat. Among the symptomatic respondents, only four (31 %) were classified as cases. Significant differences were observed only in the case of symptom occurrence relative to MPN and sampling site as indicated by p-values (0.0004 and 0.003, respectively) less than 0.05. The presence of E. coli in significant concentration and culturable Vibrios in all of the four water samples indicated poor bathing water quality of Villa Beach which may pose health risks among swimmers. The incidence of symptom occurrence or health risks was positive as reported by some of the respondents in the follow up survey. Positive relationship was observed only in the case of symptom occurrence with variables sampling site and MPN for E. coli. Symptom occurrence was found out to be more likely relative to the sampling site, and that it increases with E. coli concentration present in water samples.
Japanese involvements in the Southeast Asian Fisheries Development Center - Aquaculture Department (SEAFDEC-AQD)
Abalajon, Ma. Kristel S. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2011-04)
The Southeast Asian Fisheries Development Center has been acknowledged as one of the leading institutions in aquaculture industry by the scientific community contributing to the development of the fishing industry in the Philippines and in Southeast Asian region as a whole. Its success can be partly attributed to the assistance given by the Japanese. This study entitled — Japanese Involvement in Southeast Asian Fisheries Development Center Aquaculture Department (SEAFDEC-AQD) and in the Philippine Fishing Industry was undertaken to support the claim that Japanese assistance to the Aquaculture Department mutually beneficial to Japan and the Philippines and the rest of the SEAFDEC Member Countries. High ranking staffs and scientists were interviewed to counter check the data collected in various publications. Findings revealed that Japanese assistance to the Aquaculture Department were motive driven however benefited Japan, the Philippines and Member Countries of SEAFDEC. It can be concluded that the existence of Japan as a member country greatly influence the operations of AQD.
Larval rearing of Scylla serrata Forskal: The effect of Artemia salima and Brachionus plicatilis as food on the growth and survival of zoea
Apote, Ma. Noemi G. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1996-11)
A study was conducted to find out tile effects of Artemia, Brachionus and a combination of Anemia and Brachionus as food on the survival of mudcrab Scylla serrata Forskal zoea and the ability of the zoea to metamorphose to megalopa stage. The study composed of three experiments. Each experiment lasted for 21 days. Results showed highest mean percent survival (66.7±2.03) on zoea fed with a combination of Anemia and Brachionus at a feeding density of 5 individuals/ml each. When the density of Brachionus was increased from 5 individuals/ml to 12 individuals/ml in the combination diet, there was an increase in the survival of the zoea to 68.9±2.41. Highest metamorphosis from zoea to megalopa was also obtained on those fed with a combination of Anemia and Brachionus. This combination diet also gave the shortest number of days for the zoea to metamorphose to the next stage which also resulted to the least number of days for megalopa production to start. The study also revealed that larvae ted with a combination of Anemia and Brachionus reared at 30 ppt salinity got high survival on the first three days of culture, however starting on the 4th day up to the 15th day of culture, those reared at 28 ppt obtained higher survival although not statistically different from the other salinities tested.