Undergraduate Theses

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14583/13

Browse

Now showing 1 - 20 of 177

A benchmark survey on the cyanide and protein content in the leaves and roots of cassava (Manihot esculenta Crantz) in Iloilo
Bacolod, Hanny Joy C. (Division of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Visayas, 2006-04)
The cyanide and protein content of the roots and the leaves of the cassava samples were determined. Samples were taken from five locations, namely, Mambatad, Miagao; Poblacion, Lambunao; Ambarihon, Tubungan; Naclub, Miagao; and Calampitao, Miagao. This study shows the levels of cyanide and protein in the roots and leaves of the cassava samples from the five selected locations in the province of Iloilo only. It does not establish any concrete conclusion on the cyanide and protein content of the cassava in Iloilo. Based on the results, the amount of cyanide varies among parts of the plant with an average of 229 ppm in the leaves and 100 ppm in the roots. It also varies among varieties in the same location, the white root variety having 125 ppm cyanide in the roots and 202 ppm in the leaves, while the yellow root variety has an average of 58.2 ppm cyanide in the roots and 263 ppm in the leaves. Different locations also have different levels of cyanide. Cyanide content also decreases in the roots as the period of storage is prolonged. The level of cyanide in the white root variety from Lambunao decreased by 17.1% on the 2nd day and 61.5% on the 3rd day, while the yellow root variety, also from Lambunao, decreased by only 4.3% on the 2nd day and 30.1% on the third day. Total soluble protein in the leaves (0.12%) of the samples is higher than in the roots (0.11%) . Among the roots, the amount of protein varies. It was found out that there is a significant correlation (0.750 at 0.05 level) between the amount of protein in the roots and that in the leaves of the samples.
A community study of mangroves in Brgy. Caliling, Cauayan, Negros Occidental
Barredo, Sherry Marie P. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2000-03)
The species distribution of the mangrove community in Brgy. Caliling, Cauayanz Negros Occidental was studied using the transect line plot method with three plots for each of four stations established. Both stations 1 and 4 were located landward, while stations 2 and 3 were located seaward. Community structure Is described in terms of the following parameters; basal area, number of trees per hectare, importance value, relative density, relative frequency, frequency of species and relative dominance. Variations in these parameters are correlated with physico-chemical factors such as the air, water and soil temperature, water depth, salinity and pH. Ten true species were found In the mangals of the coastal environment of Brgy. Caliling, Cauayan, Negros Occidental. They were Avicennia aiba, Avicennia Janata, Avicennia manna, Campostemon phiiHpinensis, Excoecaria agallocha, Osbomia octodonta, Nipa fruticans, Brugeria cylindrica, Rhizophora apiculata and R. mucronata. Standard Basal Area was highest in Station 3 with 7.54 m/ha. In terms of zonation, the landward zone was found to have the highest Stand Basal Area with 11.15 m/ha. Station 2 had the highest density value of 33.92 %, whereas in terms of zonation, both the middle and seaward zone had the density value of 36.20 %. Avicennia marina was found to be the most dominant species and was observed to occupy sites with hypersaline conditions, in silty clay to muddy substratum, which may or may not be inundated by tidal flow most of the time.
A comparative study of issue and candidate orientation of voters in rural and urban barangays
Azzaraga, Josephine D. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1996-03)
Voting is the most important political activity, and for most Filipinos the only means of political participation, engaged in by citizens in a democratic society. Thus, it is important to examine the degree of importance given by voters towards issues as opposed to candidates' ascribed personal traits and achievements and their (the voters’) particularistic interests’ when they cast their votes. The locale, which is either rural or urban, may possibly show different degrees of these influences on the voters. This study, therefore, seeks to determine the level of issue and candidate orientations of voters in rural and urban barangays. The study also aims to compare the level of issue and candidate orientations of voters from rural and urban barangays. Given these objectives, a survey was conducted in the rural barangays of Bacauan, Indag-an and North Kirayan in Miag-ao, Iloilo and the urban barangays of San Pedro, Rizal and Rizal Pala-pala II in Iloilo City with a total of 100 respondents. A questionnaire which contains issue and candidate orientation scales was used. These scales were designed to elicit the issue awareness and concern of the respondents when they chose their candidate for president in the 1992 election as well as the respondents' attraction towards candidates when they voted The findings of the study reveal that voters in rural barangays are moderately issue- oriented. The study also found a majority of the voters from urban barangays to be moderately issue oriented. Among rural voters, the level of candidate orientation was found to be moderate. Voters from urban barangays were found to have a moderate level of candidate orientation. Results of the study using the Mann-Whiney U test showed that voters from rural barangays were no less issue-oriented than those from urban barangays. Also, no difference was found in the level of candidate orientation of rural and urban voters. Results suggest that attitudes towards candidates and issues in national elections are not affected by the locale of the voter.
A comparison of spelling in texting and formal writing
Barrientos, Shiela Rose Z.; Vencer, Ruby Jean A. (Division of Humanities, College of Arts and Sciences, University of the Philippines Visayas, 2011-03)
This study, entitled A Comparison of Spelling in Texting and Formal Writing, shows the similarities and differences in spelling by high school students on mobile communication and in formal writing. It also provides educators basic information on whether or not they should worry over the spelling skills of students. And it will somehow shed light on the ongoing debate as to the perceived influence of text messaging on the writing skills of students. The respondents of the study were fourth-year students of Palaca-Damilisan National High School. Using essays and text messages written and composed by the high school students, the researchers aimed to describe how high school students construct messages using cellular phone, find out their commonly misspelled words in English language based on the set of words provided by the researchers, and finally to compare the way high school students spell words in text messaging and formal writing. Using the Theory of Planned Behavior of Ajzen (2006) that shows how a person’s beliefs, the social pressure to conform to the wishes of others, and perceived ability to carry out the action can affect his behavior such as spelling words in text messaging and formal writing. After the necessary data were gathered, the researchers found that majority of the respondents scored low in spelling in text messaging and scored average in the formal writing. Twenty or 48% has a low score both in text messaging and formal writing. Only two respondents got an average score both in text messaging and formal writing, while only one rated high in formal writing but scored low in text messaging. The researchers found out that in text messaging, five of the 15 words in the list were consistently misspelled while in formal writing, the respondents spelled properly all the words except for one, which is irresistible. In addition, respondents used contractions, numbers and symbols as substitute for letters and omitted some vowels in text messaging, while in formal writing the respondents do not substitute letters with numbers. The researchers recommend to conduct more studies on the perceived influence of technological advancement such as cellular phone on the word construction in formal writing, improve the questionnaire so as to satisfy other elements stated in the Theory of Planned Behavior, formulate survey questions that will measure the respondents’ English language skills, conduct a study that would compare the spelling skills of cellular phone and non-cellular phone users, and conduct the study on a group of respondents who are cellular phone users and have a high competence in the English language.
A comparison on the effect of light on melanophore differentiation in the larval stage of frog, Hyla sp.
Araneta, Merrie Celine E. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1996-03)
Three set-ups were exposed to different experimental conditions namely: set-up A. under prolonged light: set-up B. under prolonged darkness: set-up C. under normal condition. Observation was done under the dissecting microscope from the first appearance of melanophores until the migration was stabilized. The pattern of differentiation was observed to be the same in all set-ups. only the distribution and the color intensity differred. B was the darkest and C was the lightest. It only showed that light could not, alter the pattern of melanophore differentiation, only the distribution.
A content analysis of 12 episodes of the radio comedy-drama Toyang Ermitanya
Bantiquete, Theola H.; Galanza, Siena Therese M.; Gersabalino, Jeniffer G. (Division of Humanities, College of Arts and Sciences, University of the Philippines Visayas, 2008-03)
This study “A Content Analysis of the Radio-Comedy Drama Toyang Ermitanya” aims:(1) to identify the various issues presented in the 12 episodes of the drama (Episodes 256-267, aired in July 2003); (2) to classify the issues into different categories such as political, social, economic, religious, educational and others (gender issues); (3) to determine which of the six categories are frequently raised, and; (4) to determine whether or not the drama reflects social reality. The researchers used a sell-devised descriptive code sheet in analyzing and categorizing the issues that are presented in the drama. They set six categories where each issue would fall. Percentage distribution was used to determine which among the six categories is frequently raised. They also provided actual samples of the issues under each category, quoted in the Hiligaynon language with its corresponding translation in English. Researched on the events that happened in July 2003--the time when the 12 episodes were aired over Bombo Rady Iloilo— were then related to the issues that were presented in the 12 episodes of the drama in order to determine whether or not Toyang Ermitanya reflects social reality. This study is significant for it provides an in-depth treatment of the societal issues, thus, making people aware of the prevalent issues confronting the society at the same time. Second, it serves as an important tool in analyzing the role of media in presenting various issues of the society in a manner that almost everyone can relate to. In the process, it will guide media practitioners in producing a satirical program that will catch the interest, and at the same time, meet the need of the society in terms of information and entertainment. The researchers identified 163 issues that were raised in the 12 episodes of the drama Toyang Ermitanya. Results of the study show that social issues are frequently raised in the drama. It is followed by political, economic, religious, others (gender issues), and finally, educational which got a zero percentage. Using the Cone-effect theory, the study shows that Radio Comedy-Drama Toyang Ermitanya reflects social reality. The study would have been better if the researchers interviewed the producer/ scriptwriter of Toyang Ermitanya. For future researchers, the researchers recommend that research on another radio drama be done to further validate this study’s claim that radio dramas are not only for entertainment, but, are also a good source of information and reflect social realities.
A critical analysis of the movie "Bikini Open"
Aportadera, Ela Vanessa; Azarcon, Rudylyne; Baldeviso, Donna Marie; Novelo, Fiona; Punsalan, Jona Marie (Division Humanities, College of Arts and Sciences, University of the Philippines Visayas, 2005-10-21)
This study entitled "A Critical Analysis of the Movie Bikini Open," will find out whether the said film affirms the real state of Philippine Mass Media. The study aims to identify the issues on Phi lippine media depicted in the movie, point out the issues confronting media in the Philippine society, determine the similarities and differences of the issues depicted in the movie with the current state of Philippine media and society, analyze the issues reflected in the movie in the context of Philippine media and society, and point out the implications of the movie on the Philippine media and society. Twelve professionals from Iloilo City coming from the academe, television, radio, and print media and the film's director served as respondents of the study. The study used Critical Theories of Communication in analyzing the film in the context of Philippine media and society. Results of the study indicate that Philippine media tends to serve the interest of the dominating class such as advertisers and media owners who exercise power over those below them intheir pursuit of economic interests. Philippine mass media is leaning towards the practice wherein corporate responsibility takes over social responsibility. With competition, the media resorted to sensationalism and exploitation. Moreover, media content is used as a commodity in order to sell. Finally Philippine media serves as a potent force in shaping the way of life of the people. Although such ills in the media occur, this does not generally speak for the entire Philippine mass media. Thus, this study concluded that the film "Bikini Open" partly affirmed the state of Philippine media in reality.
A descriptive study on how adolescents respond to value-bearing communication
Asong, Mariver A.; Bobe, Minnie Marie M.; Guadalquiver, Nanette L.; Jalover, Carol P.; Niembra, Lorlyn C.; Rasmo, Anna Vina Lisa L.; Salazar, Ma. Jonalyn U.; Tupas, Euridice L. (Division of Humanities, College of Arts and Sciences, University of the Philippines Visayas, 1995-03)
This study is an attempt to re-validate the study framework of Dr. Jacobson Kliatchko, which he used in his dissertation entitled, Communication and Attitude Development: A Philosophical Perspective. The framework deals with the development of attitudes after exposure to value-bearing communication. Because this study does not deal with the cause and effect relationship, the variables are rather called as components. The components of such study framework are: environment, value-bearing communication, and attitude. In this study, the environment is Marillac Academy. The value-bearing communication is the module on good study habits. And the attitude is the study habits of the respondents. needed data. Purposive sampling was used to determine the respondents. The essays and interviews with the students and the questionnaires filled up by the parents were the instruments designed to elicit the needed data. The general objective of this study is to determine how adolescents respond to value-bearing communication. Results reveal that although most of the respondents did not follow their study schedule, all of them have allotted time for their studies as reflected by the filled up observation sheets accomplished by the parents. This suggests that there is favorable response on the part of the respondents towards value-bearing communication. The study framework of Dr. Kliatchko that was used in the study shows that there is indeed an attitude formed when a value-bearing communication is inputted to a certain environment.
A historical analysis of Pintado (Euphorbia heterophylla)
Alcarde, Annabelle Z. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-03)
A histochemical study on Euphorbia heterophylla, a shrubby plant of the Family Euphorbiaceae was performed to screen the plant for the presence of medically important substances that could be responsible for its curative powers. Results of the analysis showed that aldehydes are highly detectable in the stem (xylem and phloem); moderately detectable in the leaf (xylem), stem (epidermis and vascular cambium), root (phloem) and flower (ovary); and slightly detectable in the flower (calyx). Alkaloids are moderately detectable in the stem (xylem, vascular cambium, and phloem) and slightly detectable in the leaf (xylem, vascular cambium, epidermis, and phloem). Amygdalin is highly detectable in the leaf (xylem) and stem (epidermis); moderately detectable in the root (phloem), and fruit (seed and pericarp); and slightly detectable in the root (xylem) and the flower (ovary). Arbutin is highly detectable in the xylem of the leaf and pericarp of the fruit; moderately detectable in the phloem of the leaf and vascular cambium of the stem; and slightly detectable in the stem (xylem, phloem, and epidermis), root (xylem), and flower (ovary). Saponins are highly detectable in the leaf (xylem, phloem, and epidermis), (xylem), root (xylem and phloem), flower (calyx and ovary) and fruit (seed). Formic acid is moderately detectable in the leaf and stem (epidermis), and slightly detectable in the stem and root (xylem). Oxalic acid is slightly detectable in the stem (phloem). Tannins are highly detectable in the stem (xylem and phloem) and fruit (pericarp); moderately detectable in the leaf (xylem and phloem) and fruit (seed); and slightly detectable in the stem (epidermis).
A novel water-in-oil-in-water emulsion to encapsulate antibacterial agents
Balcarcel, Angelica V. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2018-06)
Encapsulating bioactive compounds can protect them from adverse conditions. This study aimed to encapsulate bioactive agents in a stable multiple emulsion system using a material that can be recovered easily from a product abundant here in the country. Results of the study revealed that the method of encapsulation used is effective in encapsulating and releasing the bioactive compound encapsulated. The antibacterial activity determination showed that encapsulated chlorhexidine is effective against both S. aureus and E. coli bacteria. Further investigation was made by encapsulating aqueous guava (Psidium guajava) extract in the same emulsion system (water-in-oil- in-water) using recovered coconut milk protein (CMP) as a gelling agent. The evaluation of the stability of the emulsions revealed that the treatment with 40% guava extract in the aqueous phase of its primary emulsion (Emulsion 3) is the most stable among the other treatments. This treatment showed the smallest droplet size range and most uniformed droplet sizes. The creaming index of this treatment also showed the lowest values after three (3) weeks of storage. On the other hand, the treatment with 60% guava extract in the aqueous phase of its primary emulsion (Emulsion 4) appeared to be the most stable against heating. However, no antibacterial activity was observed for any of the emulsion systems both S. aureus and E. coli bacteria. Additional test revealed that the bioactive compound present in the plant extract was disrupted by the sonication process
A preliminary investigation of the antibacterial activity of the alkaloidal constituent of Hyptis suaveolens Poit (Family Labiatae)
Alcayde, Glenn G. (Division of Physical Sciences & Mathematics, College of Arts and Sciences, University of the Philippines Visayas, 1999-03)
This study determined the effectiveness of the alkaloidal constituent on the leaves ofHyptis suaveolens against the four bacteria namely S. aureus, B. subtil is E. coli, and P. aeruginosa, and two fungi namely C. albicans and S. cerevisae. The alkaloidal constituent was first detected using the Culvenor-Fitzgerald method. It was then separated in the form of an alkaloidal residue. The residue was separated further using column chromatography. This process resulted in three fraction isolates.These three fraction isolates were purified and determined their physical properties. Finally, each fraction isolate was prepared into three concentrations, 100%, 50%, and 25%. The isolates of three concentrations were then tested on six microorganisms and growth of inhibition was measured. Results showed that the three fraction isolates in three different concentrations exhibited no antibacterial and antifungal effect
A preliminary study on the extraction of alginic acid from three species of brown algae: Sargassum polycystum, Sargassum cristaefolium and Turbinaria sp. in Taklong Is.,Guimaras
Adelantar, Geoffrey V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1996-04)
Three species of brown algae were harvested in Taklong Is., Guimaras. These were Sargassum polycystum, Sargassum cristaefolium, and Turbinaria sp. These brown algae were subjected to physical and chemical treatment to extract and compare the amount of their alginic acid in the form of sodium alginate. Four seaweed collections for extraction were made between August to December, 1995. Values obtained during the first three extractions gave almost similar results. S. polycystum exhibited the highest percentage yield of sodium alginate with a mean of 22.84%. It was followed by S. cristaefolium at 22.21%. Turbinaria sp. gave the lowest yield with a mean of 14.12%. The last extraction somehow showed a decrease in the percentage yield of all three samples. S. polycystum yielded just 20.00%, S. cristaefolium at 19.00% and Turbinaria sp. at just. 10.10%. Several factors may have contributed to the differences in the amount of alginic acid of the three species of brown algae. Among these are the inherent capability of the different species of brown algae to produce alginates, the thickness and sturdiness of the samples, the viscosity of the extracts, and seasonal changes.
A preliminary study on the effect of neem (Azadirachta indica) seed extract on the reproductive potential of the female Friutfly (Drosophila melanogaster)
Altea, Armee H. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-04)
Eqqs, first instar larvae, and third instar larvae of fruit flies (D. melanogaster), were treated with 0%, 10%, and 15% neem seed extract. This resulted in reduced number of adults that emerged from treated embryos and larvae. Females following treatment crossed with untreated males have reduced number of progeny compared with control (untreated) females. Treatment of extract at three stages of development indicated that fertilized eggs were most affected by the extract. Females derived from treated eggs were sterile. The number of progeny produced by females treated during first instar stage were considerably reduced compared to controls. On the other hand, the number of progeny produced by females treated during third instar stage was not significantly different from controls. The results suggest that neem seed extract has active components which can reduce the reproductive potential of female fruit flies exposed to the chemicals particularly at the early stages of development.
A study of the performance of the marketing intermediaries at the Iloilo fishing port complex
Acanto, Jan B. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1989-03)
This research study presents an analysis on the performance of the marketing intermediaries at the Iloilo fishing Port Complex. The following types of intermediaries are involved in marketing fish landed at IFPC: brokers, wholesalers, bulanteros, and retailers. Brokers assemble and dispose the fish of producers while the wholesalers, bulanteros and retailers create the forms, time and place utilities. The results show that fish marketing intermediaries perform functions which are vital in the fish marketing system. Average costs per week were ₱13.67, ₱46.54 and ₱77.26 per tub for the brokers, wholesalers, and retailers, respectively. The average volume handled per week is 4,641.17 kilograms. Cost and returns analysis for all intermediaries showed that the average not return above total cost is ₱2.36 per kilogram. The average total income above total cost is ₱10,953.18 per week. The rate of return to capital, operator’s labor, and entrepreneurship is 14.49 percent. Efficiency of each type of intermediary _ could not be measured due to the fact that the sample size of each type is not sufficient to obtain significant results. In relation to this, a further study that could measure the efficiency of each intermediary is recommended by the researcher.
A study on spawning induction of Imbaw Anodontia edentula (Linne, 1758)
Bacaro, Teressa Mae D. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2008-03)
The mud clam, Anodontia edentula, was induced to spawn using extract from ripe and developing gonads. Clams responded to the spawning stimulus by extension of the foot, swelling and extension of the mantle, swirling of the whole body, rapid opening and closing of the valves, ejecting of white material from the excurrent siphon, and subsequent contraction of the adductor muscles. Successful spawning of all individuals occurred with the use of gonad extract. Developing homogenized extract was not as effective in inducing the broodstock to spawn. Gametes were released through the excurrent siphon located at the posterior end.
A study on the performance of the regulatory functions of the Kapisanan ng mga Broadcaster sa Pilipinas Iloilo Chapter
Advincula, Rebecca (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1987-03)
Generally, regulations are viewed as important factors in the functions of an industry or organization. Like any other industry, broadcasting needs to he regulated. The Kapisanan ng mga Brodkaster sa Pilipinas is a self-regulatory body which implements regulations affecting the programming operations of the Broadcast stations. However, not all regulations are said to be followed by the stations. It is in this regard that this study was conducted. Three factors were considered in finding out whether KBP regulations are important to the operations of the stations: the importance and necessity of these regulations; the capability of the KBP in implementing these regulations; and the extent of public participation between KBP and its member stations. The necessity level was determined by a study of each regulatory function, the activities covered by these regulations, and the relative need of the stations to these regulations. The capability level was determined by a study on the frequent observance of the stations to the regulations, the objectives of the respondent stations, and the major output, organizational structure, and budget of the KBP as a regulatory body. Public participation was determined by a study of the frequency used modes of interaction involved in the implementation of the regulations, the problems met in the process, and the respondent stations' rating/assessment of the KBP. The study made use of the one-shot survey design with interview schedule and use of questionnaires as main instruments. The respondents for the KBP are the station managers, while respondents for the regulated are the production staff of the local stations. A total of twelve stations were taken as respondents for the study. Results showed too many regulations are imposed by the KBP and are not strictly followed by the stations. Regulations that are mostly followed are those which pertain to the presentation of news while those often violated pertain to public commentaries and issues, the presentation of materials regarding sex and violence and the airing of commercials. Results showed that these regulations are necessary to maintain a certain level of excellence and professionalism in the broadcast industry, Likewise, the KBP is capable of implementing regulations to the station , as shown by the constant interaction between the regulator and the regulated.
A survey of the catch composition of fyke nets in the Iloilo river
Andrada, Kristina Abelaine D. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2000-04)
A survey of the catch composition of fyke nets in the Iloilo River Estuary during the months of August 1999 and February 2000 show that catches are dominated by Metapenaens crisis and ilossygobius sp. The 18 species caught are : 9 fish species, 5 shrimp species, and 4 crab species. The index of similarity 0.3 between the monthly samples is low which means that there is minimal degree of similarity between the species composition of the catches. There is no significant difference between the total catch volumes per day for the months of August 1999 and February 2000 which means that the seasons do not affect the volume of the catch.
A taxonomic survey of epiphytes on Enhalus acoroides in Ali Cove, Taklong Island, Nueva Valencia, Guimaras
Alojado, Ezra Nelson E. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1996-06)
Epiphytes were collected from the blades of the seagrass Enhalus acoroides which were harvested from Ali Cove, Taklong Island, Guimaras. 20 epiphytic algae were identified with the aid of taxonomic keys. Of these, five belong to the Cyanophyceae, two to the Chlorophyceae, one to the Phaeophyceae, eight to the Rhodophyceae and four to the Bacillariophyceae. Thirteen specimens were identified to the species level and seven to the genus level only. Measurements of temperature, salinity and pH were taken at the study site during the collection
Academic performance of UPV Iloilo High School students admitted under the democratized admission scheme
Apalacio, Reymund D. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1993-04)
As its contribution to the enhancement of the social welfare of people, especially the poor, in a poor developing country like the Philippines, a government university like UP has embarked on a policy democratized education which includes the scheme of democratized admission at the UPV Iloilo High School. This paper undertook action research, which is the applications of tools and methods of social science to immediate, practical problems, to determine the academic performance of economically poor students admitted under the new, democratized admission scheme of the UPV Iloilo High School. It was found out that generally they perform as well as their better-off counterparts under the old scheme (with tuition fees).
Acetone -precipitated proteins in cassava (Manihot esculenta Crantz) tubers : Isolation and partial purification
Albacete, Rose Margaret F. (Division of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Visayas, 2006-04)
Cassava (Manihot esculenta Crantz) is a low-protein starchy staple. In some countries, such as South Africa, cassava bread serves as the major, if not the only, food consumed for a long period of time. This diet causes a disease called protein-energy malnutrition. The total soluble proteins in cassava tubers were extracted using 200 mM Tris- HC1 pH 8.2. Protein fractions were precipitated out at different proportions using acetone (1:1, 1:2, 1:3 crude extract: acetone ratio). Bradford Standard Assay showed that 1:1 crude protein-acetone contained the most amount protein (1.02 x ₋ g/mL protein). This sample and the dialyzed crude sample were both run in gel filtration column in which the eluted fractions generated two peaks each. The highest concentration of the purified protein was 2.67 x 10 5 g/mL protein. The pooled fractions, which made up the peak, were run in SDS-PAGE which revealed bands with molecular weights of 40, 66, 87 and 116 kDa comprised the crude sample (not dialyzed), while the dialyzed sample contained bands with molecular weights of 76 and 87 kDa. The number of proteins decreased after gel filtration.