Undergraduate Theses
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Item The growth and yield performance of oyster mushroom (Pleurotus ostreatus Fries) using fish wastewater as fertilizerArroyo, Karen T. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2000-04)Oyster mushroom, Pleurotus ostreatus, is an edible wood-inhabiting fungi that continues to gain popularity in the Philippines, as well as in other tropical countries. With the use of modern technology, this mushroom can be grown in the Philippines, provided sufficient moisture is present. Its present cultivation is limited, perhaps due to insufficiency of planting materials and the limited knowledge about its culture. The main objective of the study was to establish the possibility of the replacement of urea, the commonly used fertilizer for mushroom cultivation by fish wastewater. The study also aimed to compare the effects of different concentrations of fish wastewater on the growth of mushrooms and determine which treatment produced the best results. A total of five groups were used: two control groups (positive and negative - using urea and water, respectively) and three experimental groups, the latter with different concentrations of fish wastewater serving as alternative fertilizer (A: 10%, B: 20%, C: 30%). Fruiting bags were inoculated with oyster mushroom spawn, and after a month of incubation, the growing mushrooms were assessed using the parameters of number, weight, length, and diameter. Results showed that the weight of oyster mushrooms was heaviest when the substrate was sprayed with plain water. However, the number of mushrooms harvested, their length, and the width of the pilei were not affected by spraying the substrate with urea or moistening these with up to 30% fish wastewater. The conclusions reached were: 1) when using sawdust as substrate for oyster mushroom cultivation, it is best to simply use water to maintain the moisture of the material; 2) sawdust used in combination with low concentration of fish wastewater (10%) can result in good production; 3) neither nitrogen nor urea may be a critical factor for the growth of oyster mushroom on sawdust; and 4) high amount of phosphorus and low potassium content of fish wastewater are favorable for oyster mushroom growth.Item Effect of soil amendments on damping-off disease of tomatoBalofiños, Junnel B. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1998-04)Different soil amendments obtained commercially were tested for their ability to suppress damping-off disease of tomato. Compost, animal manure, inorganic fertilizer, and green manure were applied to autoclaved soil prior to addition of a pathogenic inoculum. The biological control of the damping-off disease was evaluated by monitoring disease incidence in every plot of 50 seedlings for 25 days. Compost and animal manure significantly reduced disease incidence while green manure and inorganic fertilizer did not yield significant results as compared to the nonamended control soil. Percent emergence, dry matter content, and height were not at all affected by the different treatments.Item Molluscicidal effect of quicklime, CaO on golden snail (Pomacea canaliculata)Baibado, Joewel Tarra (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1998-03)The golden snail (Pomacea canaliculata) is presently a major pest of rice in lowland and rainfed areas through out the Philippines . The problem of golden snail infestation in rice farming system includes damage to the rice plants, rice yield losses, additional expense, side effects of chemicals and destructive effect on native snails . The study was conducted to evaluate the molluscicidal effect of calcium oxide for the control of golden snails in farmlands and to determine the 96hour LC50 in order to know the lethal concentration of calcium oxide to golden snails. Calcium oxide was found to have a molluscicidal activity on golden snails The golden snails were exposed to different concentrations namely. 100 ppm. 200 ppm. 300 ppm .400 ppm. and 500 ppm Remarkable lethal effects of calcium oxide (p<0.05) has been found in 4(H) and 500 ppm concentrations which has reached 60-100 % after the 96 hour treatment. The 96-hour LC50 within the 95% confidence interval was 322.02 ppm as computed Calcium oxide is safe on rice when used as a molluscicide It performs dual function as fertilizer/soil stabilizer and molluscide. These dual effects are very significant because it means big savings on the part of the rice farmers. Considering the prevalence of this pest, farmers spend much on molluscicides and their application. With calcium oxide, the farmer has a molluscicide and soil stabilizer in one application.Item Effects of high and low protein diets on the gut evacuation rate of Tilapia niloticaAndrada, Bryan Andrew S. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1999-03)Gut evacuation rate of young Nile tilapia, Tilapia nilotica fed commercial pelleted diets containing high or low protein diet were determined in the laboratory. The decline in gut fullness of the fish with time was examined at low and high protein diets. Two mathematical models were used to express the decline in gut fullness of the fish with time. Fish fed the low protein diet followed more closely the linear model while those fed the high protein diet followed the logarithmic model. The level of protein resulted in different gut evacuation rates (GER), expressed as coefficients b of the gut fullnesstime curve. GER values were higher in either linear or logarithmic models for fish fed low protein diet than for those fed high protein diet.Item Diel feeding patterns of angelwing clam Pholas (Monothyra) orientales (Gmelin, 1970)Alojepan, Marbe Joy L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1999-04)Filtration rate of Pholas orientalis measured using the indirect suspension deletion method and monitored by direct algal cell counts. Phytoplankton Isochrysis galbana was used as food at an algal cell concentration of 2.5 x 103 cells ml ”'. Biological rhythms have been demonstrated in all animals and plants except bacteria. They control rhythmic patterns in behavior and physiology of a living organism. Biological rhythms, or oscillations, have a “period” that is measured from one arbitrary point or phase of the rhythm to the next occurrence of that phase. They are best demonstrated in a 24 h cycle or circadian cycle. There is a widely varying feeding activity in the 24h cycle of P. orientalis observed at a constant temperature of 27$C and hydrogen ion concentration of 35%o Like any other bivalves, P. orientalis has defined feeding periods equivalent to food uptake at 2400h-0300h. Such observation could be supported by a previous study by Corda stating that optimal feeding was obtained at total darkness. Periods of digestion occur shortly after the periods of feeding. P. orientalis when actively feeding were observed to have extended siphons, larger valve openings, and an inclination of 45. One primary external stimulus that affected filtration rate was the amount of light present. Biorhythms provide temporal information, such as the time of day or season of the year, which facilitates adaptation of the organism to internal or external environments.Item A taxonomic survey of epiphytes on Enhalus acoroides in Ali Cove, Taklong Island, Nueva Valencia, GuimarasAlojado, Ezra Nelson E. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1996-06)Epiphytes were collected from the blades of the seagrass Enhalus acoroides which were harvested from Ali Cove, Taklong Island, Guimaras. 20 epiphytic algae were identified with the aid of taxonomic keys. Of these, five belong to the Cyanophyceae, two to the Chlorophyceae, one to the Phaeophyceae, eight to the Rhodophyceae and four to the Bacillariophyceae. Thirteen specimens were identified to the species level and seven to the genus level only. Measurements of temperature, salinity and pH were taken at the study site during the collectionItem A preliminary study on the effect of neem (Azadirachta indica) seed extract on the reproductive potential of the female Friutfly (Drosophila melanogaster)Altea, Armee H. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-04)Eqqs, first instar larvae, and third instar larvae of fruit flies (D. melanogaster), were treated with 0%, 10%, and 15% neem seed extract. This resulted in reduced number of adults that emerged from treated embryos and larvae. Females following treatment crossed with untreated males have reduced number of progeny compared with control (untreated) females. Treatment of extract at three stages of development indicated that fertilized eggs were most affected by the extract. Females derived from treated eggs were sterile. The number of progeny produced by females treated during first instar stage were considerably reduced compared to controls. On the other hand, the number of progeny produced by females treated during third instar stage was not significantly different from controls. The results suggest that neem seed extract has active components which can reduce the reproductive potential of female fruit flies exposed to the chemicals particularly at the early stages of development.Item Effect of Physico-chemical parameters of rearing waters on survival rates of larvae and post larvae of Penaeus monodon in two hatchery systemsAlindong, Ray Lauron (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1995-03)Physico-chemical parameters such as temperature, pH, salinity, and D.O. of the small-scale and large-scale hatchery system were monitored daily for a duration of two months. The relationship between the physico-chemical parameters and the survival of the different larval and post-larval stages between the two hatchery systems was then determined. Results showed that the physico-chemical parameters obtained from the small-scale hatchery system were favorable to the survival of the larval stages of the prawn. However, the physico—chemical parameters obtained from the large-scale hatchery system were more suitable for the growth of the post-larval stages.Item A historical analysis of Pintado (Euphorbia heterophylla)Alcarde, Annabelle Z. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-03)A histochemical study on Euphorbia heterophylla, a shrubby plant of the Family Euphorbiaceae was performed to screen the plant for the presence of medically important substances that could be responsible for its curative powers. Results of the analysis showed that aldehydes are highly detectable in the stem (xylem and phloem); moderately detectable in the leaf (xylem), stem (epidermis and vascular cambium), root (phloem) and flower (ovary); and slightly detectable in the flower (calyx). Alkaloids are moderately detectable in the stem (xylem, vascular cambium, and phloem) and slightly detectable in the leaf (xylem, vascular cambium, epidermis, and phloem). Amygdalin is highly detectable in the leaf (xylem) and stem (epidermis); moderately detectable in the root (phloem), and fruit (seed and pericarp); and slightly detectable in the root (xylem) and the flower (ovary). Arbutin is highly detectable in the xylem of the leaf and pericarp of the fruit; moderately detectable in the phloem of the leaf and vascular cambium of the stem; and slightly detectable in the stem (xylem, phloem, and epidermis), root (xylem), and flower (ovary). Saponins are highly detectable in the leaf (xylem, phloem, and epidermis), (xylem), root (xylem and phloem), flower (calyx and ovary) and fruit (seed). Formic acid is moderately detectable in the leaf and stem (epidermis), and slightly detectable in the stem and root (xylem). Oxalic acid is slightly detectable in the stem (phloem). Tannins are highly detectable in the stem (xylem and phloem) and fruit (pericarp); moderately detectable in the leaf (xylem and phloem) and fruit (seed); and slightly detectable in the stem (epidermis).Item Effects of freezing and thawing on some agar parameters of Eucheuma spinosum and Gracilaria confervoidesAbelende, Ma. Orfa R. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1995-05)Agar was extracted from two agar-containing red algae species, namely Gracilaria confervoides and Eucheuma spinosum. Qualitative assessment was made on the agar extracts, which included percentage yield, gel strength, syneresis, and hysteresis range. The quality of agar extracts derived from this study was compared to commercial agar-agar. The. effects of each parameters, repeated freezing and thawing on agar were also investigated. Qualitative test showed that percentage yield from Gracilaria was higher than Eucheuma. Gel strength and hysteresis range of agar from commercial sources and Gracilaria were higher compared to Eucheuma. On the other hand, syneresis range was highest in Eucheuma agar. Repeated freezing and thawing lowered percentage yield and syneresis but increased gel strength and hysteresis of agar extracts from agar sources used.
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