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Undergraduate Theses

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Committee Member: search.filters.committeemember.Malata, Ma. Pilar Charmaine S.

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    Antioxidative properties of the fruits of selected tomato (Lycopersicon esculentum Mill.) varieties
    Baltazar, Gleza Wae D. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2016-05)
    This study was conducted to compare the antioxidative properties of greenstage fruits of selected Lycopersicon esculentum Miller varieties (Diamante, Diamante Max, and Pidada) from Barangay Durog, Miagao, Iloilo. The fresh fruit samples were extracted with 95% ethanol. The crude extracts were concentrated using rotary evaporator. The antioxidant activity of the ethanolic fruit extracts was assayed for DPPH radical scavenging activity and the IC50 values were determined. The determination of the phenolic and flavonoid contents was done employing Folin- Ciocalteu method and aluminum chloride spectrophotometric method, respectively. The results show that the fruits of the studied L. esculentum varieties exhibit DPPH radical scavenging activity. The IC50 values, however, differ significantly among the varieties. The IC50 values for Diamante (24.22 ± 1.41 g/L) and Diamante Max (25.08 ± 0.45 g/L) are comparable but are significantly lower than Pidada (29.47 ± 1.33 g/L). These findings imply that Diamante and Diamante Max varieties have stronger antioxidative capacity than the Pidada variety. Moreover, the results indicate that the antioxidative property of L. esculentum is variety-dependent. The results of the determination of the total phenolic content, expressed in mg gallic acid equivalent (GAE) per g fresh sample, are in the order of 0.16 ± 0.02 mg GAE/g for Diamante > 0.15 ± 0.02 mg GAE/g for Diamante Max and Pidada. On the other hand, the results of the determination of the flavonoid content, expressed in μg quercetin equivalent (QE) per g fresh sample, are in the order of 6.31 ± 1.27 μg QE/g for Diamante >6.21 ± 0.88 μg QE/g for Diamante Max > 5.63 ± 0.94 pg QE/g for Pidada. No significant difference in the total phenolic and flavonoid contents was found among varieties. Nonetheless, the detection of phenolics and flavonoids, known antioxidants, supports the present results on the antioxidative property of the fruits of the L. esculentum varieties studied.
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    Health risks associated with bacteriological quality of bathing waters in Villa Beach, Brgy. Sto. Niño Sur, Arevalo, Iloilo City
    Abad, Sue Ellen T.; Catinan, Mary Joy A.; Hautea, Therese Angelie T.; Mabang, Moctar M.; Narida, Leah Joy S.; Panggat, Kevin Matthew B.; Turija, Maisie Mae L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2004-04)
    A special problem submitted to Division of Biological Sciences, College of Arts and Sciences, University of the Philippines in the Visayas, Miagao, Iloilo. The study investigated the bacteriological quality, using E. coli, Salmonella spp., and culturable Vibrios as bacterial indicators, of four selected bathing waters in Villa Beach, Brgy. Sto. Nino Sur, Arevalo, Iloilo City, as well as the incidence of symptom occurrence associated with these microbes among 33 respondents. This was to determine health risks associated with bacteriological quality of bathing waters in Villa Beach. Conventional and modified laboratory procedures were used in the detection of E. coli, Salmonella spp., and culturable Vibrios, while pre-structured interview schedule was utilized in the epidemiological survey. p-value was used as statistical tool to measure association. All four water samples tested positive for E. coli and culturable Vibrios. Salmonella spp. was negative in all of the water samples. The MPN for E. coli in water sample C was 460 per 100 ml water sample, while in water samples A, B and D it was 240. Obtained MPN were above the DENR MPN standard for fecal coliform for Class SB, which is only 200. There were 52 individual participated in the study but only 33 (63 %) were able to complete the follow up interview done seven days after the actual date of survey. 18 (55 %) of the respondents were females. The mean (SD) age was 19.3 (14.5) years with an age range of 3-56 years. 11 (34 %) respondents came from sampling site C and 32 (97 %) were visitor-swimmers. Only 13 (39 %) of the respondents reported to have experienced symptoms during the follow up, and the most common symptoms reported by 10 (77 %) of the symptomatic respondents were upper respiratory tract symptoms such as colds, cough and sore throat. Among the symptomatic respondents, only four (31 %) were classified as cases. Significant differences were observed only in the case of symptom occurrence relative to MPN and sampling site as indicated by p-values (0.0004 and 0.003, respectively) less than 0.05. The presence of E. coli in significant concentration and culturable Vibrios in all of the four water samples indicated poor bathing water quality of Villa Beach which may pose health risks among swimmers. The incidence of symptom occurrence or health risks was positive as reported by some of the respondents in the follow up survey. Positive relationship was observed only in the case of symptom occurrence with variables sampling site and MPN for E. coli. Symptom occurrence was found out to be more likely relative to the sampling site, and that it increases with E. coli concentration present in water samples.