Undergraduate Theses
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Item Decomposition of sugarcane bagasse by TrichodermaBangilan, Ma. Irish P. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-04)Sugarcane bagasse, an end-product after extraction of the juice of the sugarcane, was composted with the aid of Trichoderma, a compost fungus activator which shortens the decomposition process of farm wastes. Decomposition process was observed through the changes in temperature and substrate consistency. An average of 94 days was noted for a complete decomposition of 900 kg bagasse. Analysis of bagasse compost gave 0.78% nitrogen, 4.94% phosphorus, in the form of P2O5 and 1.55% potassium, in the form of K 2O. It showed that composted bagasse is a good source of phosporus but poor in nitrogen and potassium. The compost, nevertheless, can also be a good soil conditioner.Item Karyotype analysis of garlic, Allium sativum L. (Alliales; Alliaceae)Bandiola, Ma. Florma A. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-03)The C-metaphase count of Allium sativum L. showed a chromosome number of 2n = 14, consisting of six long and eight short somatic chromosomes categorized into three distinct groups: ten m type chromosomes with centromeres at the median region, two sm type chromosomes with submedian centromere and two st type chromosomes with subterminal centromere, Mitotic index for the species was computed at 16.88%.Item Historical analysis of Hippeatrum puniceum (Var) Lam.Bañas, Shirlyn L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1996-03)A histochemical study Hipppeastrum puniceum, a bulbous plant of Family Amaryllidaceae, was performed to screen the plant for the presence of medically important substances that could be responsible for its curative powers. Results of the analyses showed that the leaf reacted positively to tests for presence of aldehydes, alkaloids, amygdalin, and arbutin; the roots tested positive for aldehydes, alkaloids, amygdalin, lecithin, oxalic acid, saponins, and tartaric acid. The bulb had aldehydes, alkaloids, amygdalin, arbutin, lecithin, saponins and tannins. On the other hand, the leaf, root, and bulb yielded negative results in the tests for presence of presence of citric acid, formic acid, lignin, and phytosterol.Item Determination of the fungal flora in the rearing water, eggs, larvae and post-larvae of Penaeus monodon in a large-scale hatchery systemBaes, Glanee F. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1995-03)The rearing water and homogenized. eggs, larvae, post-larvae of Penaeus monodon were taken front the large-scale hatchery system. These were counted, isolated and identified. Analysis was done by determining the mean, standard deviation and skewness of the data. The result showed that the skewness of the yeast and mold counts in the rearing water were 0.93 and 0.64, respectively 'while that in the homogenized samples were 0.92 and 1.09, respectively. These showed that the data were positively skewed, hence, fungi were greater in the early larval stages than in the later stages. The mean showed that yeast yielded greater counts as compared to the molds. Furthermore, rearing water have also higher fungal count than that of the homogenized prawns. The fungi identified were Aspergillus, Balanium, Bispora, Cladosporium and Phialomysis. In the study, the fungi identified were the normal mycoflora which did not adversely harm the prawn due to favorable hatchery conditions, there being no disease outbreak during the duration of the study.Item A taxonomic survey of epiphytes on Enhalus acoroides in Ali Cove, Taklong Island, Nueva Valencia, GuimarasAlojado, Ezra Nelson E. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1996-06)Epiphytes were collected from the blades of the seagrass Enhalus acoroides which were harvested from Ali Cove, Taklong Island, Guimaras. 20 epiphytic algae were identified with the aid of taxonomic keys. Of these, five belong to the Cyanophyceae, two to the Chlorophyceae, one to the Phaeophyceae, eight to the Rhodophyceae and four to the Bacillariophyceae. Thirteen specimens were identified to the species level and seven to the genus level only. Measurements of temperature, salinity and pH were taken at the study site during the collectionItem A preliminary study on the effect of neem (Azadirachta indica) seed extract on the reproductive potential of the female Friutfly (Drosophila melanogaster)Altea, Armee H. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-04)Eqqs, first instar larvae, and third instar larvae of fruit flies (D. melanogaster), were treated with 0%, 10%, and 15% neem seed extract. This resulted in reduced number of adults that emerged from treated embryos and larvae. Females following treatment crossed with untreated males have reduced number of progeny compared with control (untreated) females. Treatment of extract at three stages of development indicated that fertilized eggs were most affected by the extract. Females derived from treated eggs were sterile. The number of progeny produced by females treated during first instar stage were considerably reduced compared to controls. On the other hand, the number of progeny produced by females treated during third instar stage was not significantly different from controls. The results suggest that neem seed extract has active components which can reduce the reproductive potential of female fruit flies exposed to the chemicals particularly at the early stages of development.Item Effect of Physico-chemical parameters of rearing waters on survival rates of larvae and post larvae of Penaeus monodon in two hatchery systemsAlindong, Ray Lauron (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1995-03)Physico-chemical parameters such as temperature, pH, salinity, and D.O. of the small-scale and large-scale hatchery system were monitored daily for a duration of two months. The relationship between the physico-chemical parameters and the survival of the different larval and post-larval stages between the two hatchery systems was then determined. Results showed that the physico-chemical parameters obtained from the small-scale hatchery system were favorable to the survival of the larval stages of the prawn. However, the physico—chemical parameters obtained from the large-scale hatchery system were more suitable for the growth of the post-larval stages.Item A historical analysis of Pintado (Euphorbia heterophylla)Alcarde, Annabelle Z. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-03)A histochemical study on Euphorbia heterophylla, a shrubby plant of the Family Euphorbiaceae was performed to screen the plant for the presence of medically important substances that could be responsible for its curative powers. Results of the analysis showed that aldehydes are highly detectable in the stem (xylem and phloem); moderately detectable in the leaf (xylem), stem (epidermis and vascular cambium), root (phloem) and flower (ovary); and slightly detectable in the flower (calyx). Alkaloids are moderately detectable in the stem (xylem, vascular cambium, and phloem) and slightly detectable in the leaf (xylem, vascular cambium, epidermis, and phloem). Amygdalin is highly detectable in the leaf (xylem) and stem (epidermis); moderately detectable in the root (phloem), and fruit (seed and pericarp); and slightly detectable in the root (xylem) and the flower (ovary). Arbutin is highly detectable in the xylem of the leaf and pericarp of the fruit; moderately detectable in the phloem of the leaf and vascular cambium of the stem; and slightly detectable in the stem (xylem, phloem, and epidermis), root (xylem), and flower (ovary). Saponins are highly detectable in the leaf (xylem, phloem, and epidermis), (xylem), root (xylem and phloem), flower (calyx and ovary) and fruit (seed). Formic acid is moderately detectable in the leaf and stem (epidermis), and slightly detectable in the stem and root (xylem). Oxalic acid is slightly detectable in the stem (phloem). Tannins are highly detectable in the stem (xylem and phloem) and fruit (pericarp); moderately detectable in the leaf (xylem and phloem) and fruit (seed); and slightly detectable in the stem (epidermis).Item Effects of freezing and thawing on some agar parameters of Eucheuma spinosum and Gracilaria confervoidesAbelende, Ma. Orfa R. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1995-05)Agar was extracted from two agar-containing red algae species, namely Gracilaria confervoides and Eucheuma spinosum. Qualitative assessment was made on the agar extracts, which included percentage yield, gel strength, syneresis, and hysteresis range. The quality of agar extracts derived from this study was compared to commercial agar-agar. The. effects of each parameters, repeated freezing and thawing on agar were also investigated. Qualitative test showed that percentage yield from Gracilaria was higher than Eucheuma. Gel strength and hysteresis range of agar from commercial sources and Gracilaria were higher compared to Eucheuma. On the other hand, syneresis range was highest in Eucheuma agar. Repeated freezing and thawing lowered percentage yield and syneresis but increased gel strength and hysteresis of agar extracts from agar sources used.Item A preliminary study on the extraction of alginic acid from three species of brown algae: Sargassum polycystum, Sargassum cristaefolium and Turbinaria sp. in Taklong Is.,GuimarasAdelantar, Geoffrey V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1996-04)Three species of brown algae were harvested in Taklong Is., Guimaras. These were Sargassum polycystum, Sargassum cristaefolium, and Turbinaria sp. These brown algae were subjected to physical and chemical treatment to extract and compare the amount of their alginic acid in the form of sodium alginate. Four seaweed collections for extraction were made between August to December, 1995. Values obtained during the first three extractions gave almost similar results. S. polycystum exhibited the highest percentage yield of sodium alginate with a mean of 22.84%. It was followed by S. cristaefolium at 22.21%. Turbinaria sp. gave the lowest yield with a mean of 14.12%. The last extraction somehow showed a decrease in the percentage yield of all three samples. S. polycystum yielded just 20.00%, S. cristaefolium at 19.00% and Turbinaria sp. at just. 10.10%. Several factors may have contributed to the differences in the amount of alginic acid of the three species of brown algae. Among these are the inherent capability of the different species of brown algae to produce alginates, the thickness and sturdiness of the samples, the viscosity of the extracts, and seasonal changes.