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Undergraduate Theses

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    The growth and yield performance of oyster mushroom (Pleurotus ostreatus Fries) using fish wastewater as fertilizer
    Arroyo, Karen T. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2000-04)
    Oyster mushroom, Pleurotus ostreatus, is an edible wood-inhabiting fungi that continues to gain popularity in the Philippines, as well as in other tropical countries. With the use of modern technology, this mushroom can be grown in the Philippines, provided sufficient moisture is present. Its present cultivation is limited, perhaps due to insufficiency of planting materials and the limited knowledge about its culture. The main objective of the study was to establish the possibility of the replacement of urea, the commonly used fertilizer for mushroom cultivation by fish wastewater. The study also aimed to compare the effects of different concentrations of fish wastewater on the growth of mushrooms and determine which treatment produced the best results. A total of five groups were used: two control groups (positive and negative - using urea and water, respectively) and three experimental groups, the latter with different concentrations of fish wastewater serving as alternative fertilizer (A: 10%, B: 20%, C: 30%). Fruiting bags were inoculated with oyster mushroom spawn, and after a month of incubation, the growing mushrooms were assessed using the parameters of number, weight, length, and diameter. Results showed that the weight of oyster mushrooms was heaviest when the substrate was sprayed with plain water. However, the number of mushrooms harvested, their length, and the width of the pilei were not affected by spraying the substrate with urea or moistening these with up to 30% fish wastewater. The conclusions reached were: 1) when using sawdust as substrate for oyster mushroom cultivation, it is best to simply use water to maintain the moisture of the material; 2) sawdust used in combination with low concentration of fish wastewater (10%) can result in good production; 3) neither nitrogen nor urea may be a critical factor for the growth of oyster mushroom on sawdust; and 4) high amount of phosphorus and low potassium content of fish wastewater are favorable for oyster mushroom growth.
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    Molluscicidal effect of quicklime, CaO on golden snail (Pomacea canaliculata)
    Baibado, Joewel Tarra (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1998-03)
    The golden snail (Pomacea canaliculata) is presently a major pest of rice in lowland and rainfed areas through out the Philippines . The problem of golden snail infestation in rice farming system includes damage to the rice plants, rice yield losses, additional expense, side effects of chemicals and destructive effect on native snails . The study was conducted to evaluate the molluscicidal effect of calcium oxide for the control of golden snails in farmlands and to determine the 96hour LC50 in order to know the lethal concentration of calcium oxide to golden snails. Calcium oxide was found to have a molluscicidal activity on golden snails The golden snails were exposed to different concentrations namely. 100 ppm. 200 ppm. 300 ppm .400 ppm. and 500 ppm Remarkable lethal effects of calcium oxide (p<0.05) has been found in 4(H) and 500 ppm concentrations which has reached 60-100 % after the 96 hour treatment. The 96-hour LC50 within the 95% confidence interval was 322.02 ppm as computed Calcium oxide is safe on rice when used as a molluscicide It performs dual function as fertilizer/soil stabilizer and molluscide. These dual effects are very significant because it means big savings on the part of the rice farmers. Considering the prevalence of this pest, farmers spend much on molluscicides and their application. With calcium oxide, the farmer has a molluscicide and soil stabilizer in one application.
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    Effects of high and low protein diets on the gut evacuation rate of Tilapia nilotica
    Andrada, Bryan Andrew S. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1999-03)
    Gut evacuation rate of young Nile tilapia, Tilapia nilotica fed commercial pelleted diets containing high or low protein diet were determined in the laboratory. The decline in gut fullness of the fish with time was examined at low and high protein diets. Two mathematical models were used to express the decline in gut fullness of the fish with time. Fish fed the low protein diet followed more closely the linear model while those fed the high protein diet followed the logarithmic model. The level of protein resulted in different gut evacuation rates (GER), expressed as coefficients b of the gut fullnesstime curve. GER values were higher in either linear or logarithmic models for fish fed low protein diet than for those fed high protein diet.
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    Diel feeding patterns of angelwing clam Pholas (Monothyra) orientales (Gmelin, 1970)
    Alojepan, Marbe Joy L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1999-04)
    Filtration rate of Pholas orientalis measured using the indirect suspension deletion method and monitored by direct algal cell counts. Phytoplankton Isochrysis galbana was used as food at an algal cell concentration of 2.5 x 103 cells ml ”'. Biological rhythms have been demonstrated in all animals and plants except bacteria. They control rhythmic patterns in behavior and physiology of a living organism. Biological rhythms, or oscillations, have a “period” that is measured from one arbitrary point or phase of the rhythm to the next occurrence of that phase. They are best demonstrated in a 24 h cycle or circadian cycle. There is a widely varying feeding activity in the 24h cycle of P. orientalis observed at a constant temperature of 27$C and hydrogen ion concentration of 35%o Like any other bivalves, P. orientalis has defined feeding periods equivalent to food uptake at 2400h-0300h. Such observation could be supported by a previous study by Corda stating that optimal feeding was obtained at total darkness. Periods of digestion occur shortly after the periods of feeding. P. orientalis when actively feeding were observed to have extended siphons, larger valve openings, and an inclination of 45. One primary external stimulus that affected filtration rate was the amount of light present. Biorhythms provide temporal information, such as the time of day or season of the year, which facilitates adaptation of the organism to internal or external environments.
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    Evaluation of feeding performance of early siganid, Siganus guttatus, larvae fed with mixed zooplankton
    Andrade, Maria Shiela S. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1999-04)
    Siganus guttatus is an economically important rabbitfish for commercial farming. It is very important to evaluate their feeding performance particularly the effects of different diets for this larvae and to determine their growth and survival after being fed on different diets. Newly-hatched siganid, Siganus guttatus, larvae were reared in 500-L fiberglass tanks and fed either on copepod nauplii and rotifers alone or combination of both. Copepod nauplii proliferated in tanks consisted mainly of Pseudodiaptomus sp., and Acartia sp. Siganid larvae successfully commenced feeding (feeding incidence) on early stage copepod nauplii even if their abundance was low. Selective feeding ability of larvae seemed to start from day 6 and the larvae thereafter preferred to feed nauplii than rotifers. Survival of the siganid larvae was significantly higher when fed with with combination of rotifers and copepod nauplii compared to those fed with either rotifers or copepod alone. The growth of the larvae however are the same for all treatments. Results of this study suggest that coastal calanoid copepods of the genus Pseudodiaptomus sp. and Acartia sp. could be reproduced in tanks and their nauplii can be used as food for siganid larval rearing.
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    Bacteriological analysis of seawater from San Salvador to Belen bay areas in Banate Bay, Banate, Iloilo
    Abancio, Celyn Ann L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines in Visayas, 2000-04)
    This study was conducted at Banate bay, Banate, Iloilo from a point from Belen to San Salvador. Two samplings were taken one week apart and was analyzed for the Most Probable Number (MPN) per ml coliform count The Multiple fermentation tube technique showed that in Poblacion approximately 100 meters from the shore had the lowest MPN values for both samplings which was >3 MPN per ml. The highest count of greater than or equal to 2400 MPN per ml was obtained in sites Belen-10, Belen-50, and Bularan-10 meters away from the shore. As the distance from the shore increases, the MPN values usually decreases, showing that the area near the shore would exhibit high coliform counts than the are further away from the shore. The tide and the weather may have affected the coliform counts because of the differences of MPN values.