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Undergraduate Theses

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    Comparison of direct microscopy and concentration techniques using household bleach for the detection of acid-fast bacilli in sputum
    Bernabe, John Marvin S.; Biaoco, Beverly G.; Castillanes, Melvic A.; Fernandez, Ia Jean F.; Geraldoy, Ruffa V.; Molina, Ryge Albert D.; Pluma, Zyra April D.; Que, Agnes Karen B.; Servidad, Mercy Jeane C.; Tamayo, Kyria Mae Marre P. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2008-03-29)
    Several published studies have demonstrated that the sedimentation and centrifugation concentration techniques which utilize sodium hypochlorite are more efficient in detecting acid fast bacilli in sputum smears compared to the conventional direct smear method. This study was conducted to (1) compare the proportion of AFB-positive samples by direct microscopy with the household bleach centrifugation concentration technique; (2) compare the proportion of AFB-positive samples by direct microscopy with the household bleach sedimentation concentration technique;(3) compare the proportion of AFB-positive samples by household bleach sedimentation with the centrifugation concentration techniques; and (4) compare the cost of the direct smear method, centrifugation concentration technique, and sedimentation concentration technique for detection of AFB. Sputum specimens were collected from TB-DOTS clinics in Miag-ao and San Joaquin, Iloilo and from Medicus laboratories in Iloilo City. The specimens were then processed through the direct smear method, centrifugation concentration method, and sedimentation concentration method. Out of 174 sputum samples included in this study, results revealed that (1) there was no significant difference in the proportions of AFB-positive slides detected using the direct microscopy and centrifugation concentration technique; (2) there was no significant difference in the proportions of AFB-positive slides detected using the direct microscopy and sedimentation concentration technique; (3) there was no significant difference in the proportions of AFB-positive slides detected using the centrifugation and the sedimentation concentration techniques; and (4) simple cost analysis of the three methods suggests that the direct smear method is the most cost efficient. The findings indicate that whichever method is used would yield almost the same proportions of AFB-positive slides. Internal quality control should be maintained throughout the laboratory work to obtain reliable results, as it is the manner of processing that determines the outcome of this study and its validity.
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    Cost and return analysis of van operation in the Municipality of Miag-ao, Iloilo
    Alvizo, Marjorie V.; Laura, Cecille R. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2003-03)
    Generally, this study aimed to evaluate or assess the profitability of van operation in Miag-ao, Iloilo. It specifically dealt with the cost and return structures of the said business. The study was conducted in the municipality of Miag-ao, which is one of the towns in the province of Iloilo. There were 11 respondents who are all van operators and at the same time members of the Miag-ao Association of Van Owners and Drivers, Inc. (MAVAD). Two of them owned or operate two vans, so in the data presented there were 13 vans. A one-shot survey using recall method was used as a source of data. An interview schedule was also utilized in gathering the needed data for the study. In analyzing the data, frequency distribution, percentage, ranking and weighted mean were employed. Likewise, cost and return analysis was used to determine the profitability of the van operation. Results of the study showed that almost half of the respondents have an age range of 30-40 years. Majority (73%) of them were males and already married. All van operators earned college degrees and have well-paying jobs. Roman Catholic was the dominant religion and most of them have or belong to medium family size. The cost and return analysis of the study showed that the average initial investment in the van operation was P 238,000. The total annual operating cost was P 434,366. Forty nine percent of it was paid for the salaries and allowances of the laborers. The annual gross revenue was P 505,210 with a net income (or pure economic profit) of P 70,844. Rate of return on investment was 30% and it will take 3.4 years for the business to recover its initial capital outlay. Based on the results presented, with positive net profit and gross revenue it is profitable to engage in this business venture.