Undergraduate Theses
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Item Moss (Brachythecium sp.) and a filamentous green algae (Oedogonium sp.) as bunker oil sorbentsBautista, Norwell Brian C. (Division of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Visayas, 2007-04)Oil spills are very catastrophic and very destructive events. One way of controlling the dispersion of oil from these spills is by the use of sorbents. The procurement of commercial sorbents can be very costly. Thus, this study analyzed and evaluated the efficiency and effectivity of moss (Brachythecium sp.) and a green filamentous algae Oedogonium sp. as substitutes for commercial sorbents. Brachythecium sp. can grow in any moisture—rich environment and Oedogonium sp. algae grow freely in fish ponds and in any body of stagnant, oxygen-poor water. Thus, both samples are very abundant in the environment. Sorption capacity results showed that the moss Brachythecium sp. and the algae Oedogonium sp. can sorb five times and seven times its weight, respectively. In comparison, Peat Sorb, a commercial sorbent, was recorded to sorb 15 times its weight (Emedco, 2007). The minimum sorption capacity of the moss and algae samples was recorded to be approximately equal to their weight. The adsorption isotherms of both samples follow the Langmuir plot for monolayer adsorption. In the hexane capillary rise experiment, hexane rose significantly in ground moss (72.25 mm in 7 min.) and unground moss (64.80 mm in 7 min.) compared with unground algae (23.52 mm in 7 min.) and (20.70 mm in 7 min.), indicating that moss is more hydrophobic. In water, capillary rise, on the other hand, unground algae is a better medium (5.76 mm in 4 min) compared to the rest (ground algae - 4 mm, unground moss - 5.29 mm, and ground moss — 4.11 mm, all in 4 min.). Results also revealed that the moss Brachythecium sp. and the algae Oedogonium sp. expressed hydrophobic characters in the hexane-water system by remaining in the hexane layer after agitation. The moisture content of the algae (14.85%) is twice that of moss (7.94%). This gives the moss an advantage in oil sorption. The lipid contents of algae (15.58%) and moss (11.47%) do not comprise the majority of their biomass, but the lipids aid in increasing the samples’ affinity for non-polar substances. The samples may not sorb as effectively as the commercial sorbents, but they are abundant in the environment, thus, a cheaper alternative. Like Peat Sorb, which was processed from the biomass of Sphagnum sp. moss, the samples may undergo the same process that increases the hydrophobicity several-fold and gives oil-biodegradation capabilities.Item A preliminary study on the extraction of alginic acid from three species of brown algae: Sargassum polycystum, Sargassum cristaefolium and Turbinaria sp. in Taklong Is.,GuimarasAdelantar, Geoffrey V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1996-04)Three species of brown algae were harvested in Taklong Is., Guimaras. These were Sargassum polycystum, Sargassum cristaefolium, and Turbinaria sp. These brown algae were subjected to physical and chemical treatment to extract and compare the amount of their alginic acid in the form of sodium alginate. Four seaweed collections for extraction were made between August to December, 1995. Values obtained during the first three extractions gave almost similar results. S. polycystum exhibited the highest percentage yield of sodium alginate with a mean of 22.84%. It was followed by S. cristaefolium at 22.21%. Turbinaria sp. gave the lowest yield with a mean of 14.12%. The last extraction somehow showed a decrease in the percentage yield of all three samples. S. polycystum yielded just 20.00%, S. cristaefolium at 19.00% and Turbinaria sp. at just. 10.10%. Several factors may have contributed to the differences in the amount of alginic acid of the three species of brown algae. Among these are the inherent capability of the different species of brown algae to produce alginates, the thickness and sturdiness of the samples, the viscosity of the extracts, and seasonal changes.Item Analysis of the lipid content of micro algae species used in Philippine aquaculture for their potential in biodiesel productionFerriols, Victor Marco Emmanuel N. (Institute of Aquaculture, College of Fisheries and Ocean Sciences, University of the Philippines Visayas, 2008-03)This study looked into the proximate lipid content of four micro algal species (Chaetoceros calcitrans, Tetraselmis tetrahele, Nannochloropsis sp., and Chlorella sp) and one macrophyte (Chaetomorpha sp.) commonly used in Philippine aquaculture. Prior to the proximate analysis using the method described by Bligh and Dyer (1959), the four micro algal species were cultured for five days in 1 L vessels fertilized with TMRL enrichment media. Proximate gravimetric analysis showed that the average lipid content of Tetraselmis tetrahele at 24.11% was significantly higher (p < 0.01) when compared to all the algal species tested. Comparing the mean lipid content of all micro algal species against that of the macrophyte also revealed a significantly higher (p < 0.01) lipid content at 17.40%. Growth rates for all the micro algal species over the culture period was also measured, with the highest growth rate exhibited by T. tetrahele at 1.15 divisions per day. Projected estimates for micro algal production yields of oil from a 1-hectare pond at 0.5 m depth were also calculated from the lipid analysis data, a showing that the highest yield of oil (7.29 tons) can be attained by culturing T. tetrahele. This number was higher than estimated reports for oil production from an area of 1 hectare dedicated to either jatropha (1.7 tons) or coconuts (5.0 tons). This makes the culture of micro algal species a potential option for producing feedstock for biodiesel production.