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Undergraduate Theses

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14583/13

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Keyword: search.filters.keyword.Antibacterial activitySDG: search.filters.sdg.SDG 15 - Life on land

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    Hydrogel composites loaded with plant extract as an antibacterial and quorum sensing inhibiting wound dressing
    Baranda, Marivic M. (Division of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2018-06)
    Delayed wound healing is often caused by bacterial infection. Furthermore, the persistent inflammation brought by the formation of biofilm via quorum sensing causes pain and discomfort. In a novel effort, this study fabricated an antibacterial and quorum sensing inhibiting hydrogel dressing loaded with plant extracts. KC-PVP hydrogel composites were loaded separately with lg/mL C. longa and H. durvillei extract under optimized conditions. The hydrogels were characterized through swelling test and FTIR analysis. It was determined that C. longa-loaded and H. durvillei-loaded hydrogels have a maximum swelling capacity of 175% and 135%, respectively. The antibacterial efficacy and the quorum sensing inhibition activity of extract-loaded hydrogels were evaluated using soft-layer agar well diffusion method. Hydrogel loaded with C. longa extract displayed 47.0 (±1.0), 45.0 (±1.0), and 32.33 (±0.58) mm mean growth inhibition against S. aureus, E. coli, and P. aeruginosa, respectively. H. durvillei extract-loaded hydrogels inhibited the growth of S. aureus, E. coli, and P. aeruginosa by 45.33 (±0.58), 43.67 (±1.52), and 33.33 (±0.58) mm in diameter, respectively. The quorum-sensing inhibition zone of C. longa extract and H. durvillei extract-loaded hydrogels were 49.63 (±0.40) and 49.37 (±1.11) mm in diameter, respectively. Overall, the extract-loaded hydrogel composites exhibited good antibacterial and quorum-sensing inhibiting activity, potentially for wound treatment.
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    A preliminary investigation of the antibacterial activity of the alkaloidal constituent of Hyptis suaveolens Poit (Family Labiatae)
    Alcayde, Glenn G. (Division of Physical Sciences & Mathematics, College of Arts and Sciences, University of the Philippines Visayas, 1999-03)
    This study determined the effectiveness of the alkaloidal constituent on the leaves ofHyptis suaveolens against the four bacteria namely S. aureus, B. subtil is E. coli, and P. aeruginosa, and two fungi namely C. albicans and S. cerevisae. The alkaloidal constituent was first detected using the Culvenor-Fitzgerald method. It was then separated in the form of an alkaloidal residue. The residue was separated further using column chromatography. This process resulted in three fraction isolates.These three fraction isolates were purified and determined their physical properties. Finally, each fraction isolate was prepared into three concentrations, 100%, 50%, and 25%. The isolates of three concentrations were then tested on six microorganisms and growth of inhibition was measured. Results showed that the three fraction isolates in three different concentrations exhibited no antibacterial and antifungal effect