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Undergraduate Theses

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Keyword: search.filters.keyword.AntibacterialSDG: search.filters.sdg.SDG 12 - Responsible consumption and production

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    Phytochemical screening, antioxidant activity, and comparative bioefficacy assessment of Camansi (Artocarpus camansi) seed extracts for in vitro antibacterial activity
    Bantayan, Andrea Marie B. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2016-06)
    This study was conducted to compare and assess the antibacterial and antioxidative properties, and to screen for the presence of phytochemical constituents in the different solvent-derived extracts of Artocarpus camansi seeds from Mintal, Davao City. Phytochemical analysis revealed the presence of alkaloids, flavonoids, glycosides, phenol, steroids, tannins, and terpenoids in the methanol extract. The antibacterial efficacy was determined by agar well diffusion method against B. subtilis and E. coli. Streptomycin was used as a standard drug for the study of antibacterial activity. Sensitivity in terms of zones of inhibition and phytochemical composition of the different extracts were also determined. Results showed that all extracts were effective against both of the bacteria tested with the pet. ether and DCM extracts showing moderate activity, and the methanol extract exhibiting strong activity against B. subtilis and E. coli. Furthermore, methanol extract of A. camansi seeds possessed strong DPPH radical scavenging activity. In the DPPH method, the pet. ether, DCM, and methanol extracts showed free radical inhibition of 18.96±0.16%, 21.62±0.14%, and 90.05±0.08%, respectively. The IC50 value obtained for the methanol seed extract at concentration 0.05 g/mL was 6.67±0.004 g/L. The current findings suggest that the phytochemicals present in the methanol extract of Artocarpus camansi seeds (alkaloids, flavonoids, glycosides, phenol, steroids, tannins, and terpenoids) may be responsible for the antibacterial and antioxidant activity.
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    Anti-inflammatory and antibacterial activities of Batuan (Garcinia binucao) fruits
    Albano, Zella Faye (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2010)
    In this study, fresh fruits of batuan (Garcinia binucao) was evaluated for its antiinflammatory and antibacterial activities. The anti-inflammatory assay using Carrageenan-Induced Paw Edema Method showed that n-hexane extracts (65.79%) and dichloromethane extracts (64.91%) exhibited anti-inflammatory activities comparable to Indomethacin, the positive control (67.11%). The antibacterial assay using Paper Disk Diffusion Method showed that crude methanolic extracts, n-hexane extracts and dichloromethane extracts at 1000pg/mL were most effective against Staphylococcus aureus. The microbial indexes were 0.39, 0.47 and 0.50 respectively. The positive control, Amoxicillin has a 3.39 microbial index. The components of the three most active extracts (crude methanolic, n-hexane and DCM extracts) from both assays were separated through Thin Layer Chromatography and subjected to phytochemical tests. Crude methanolic extracts contained alkaloids, phenolic compounds and cardiac glycosides. Both n-hexane and DCM extracts contained terpenes, alkaloids, saponins, phenolic compounds and cardiac glycosides. These bioactive compounds present may have been responsible for the antiinflammatory and antibacterial activities of the extracts.