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Undergraduate Theses

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14583/13

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Start date: 2010SDG: search.filters.sdg.SDG 3 - Good health and well-being

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    Antifungal activities of lemongrass (Cymbopogon citratus) essential oil -B-cyclodextrin inclusion complex incorporated in commercial fish feeds
    Baure, Jiaren G. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2019-06)
    During the wet season in the Philippines, fish feeds can be contaminated with mycotoxins, affecting fish production. The addition of essential oil (EO) is one of the ways to prevent this, but it is prone to physical and chemical reactions. Thus, this study aims to encapsulate lemongrass essential oil (LGEO) in B-cyclodextrin (B-CD) to produce an inclusion complex (IC) that can be used as an additive in commercial tilapia feeds to prevent or minimize fungal contamination. The inclusion complex was made using the co-precipitation method and was characterized by Fourier Transform Infrared (FTIR) Spectroscopy. The spectrum was similar to that of citral, a major component of lemongrass essential oil that is responsible for inhibiting fungi. The antifungal activities of pure and encapsulated LGEO at varying concentrations against Aspergillus species were carried out by the Agar Well Diffusion assay. Results showed that A. flavus was more susceptible in LGEO and IC compared with A. fumigatus. The tested concentration of EO could inhibit both fungi. However, it would take 50% IC to effectively inhibit the growth of both fungi. The stability of the inclusion complex in commercial fish feeds was also evaluated. The feeds were stored in an area with the appropriate conditions that could promote the growth of molds, such as temperature (>27°C), moisture levels (14%), and relative humidity levels (>70%). The results showed that the encapsulated LGEO was effective in extending the shelf life of the feeds and was best observed at a concentration of 5 g kg-1 IC. The oil remained active for nine days, wherein about 10- 40% of the feeds with 5 g kg-1 IC were covered with molds. On the other hand, about 70-100% of the surface of the feeds with essential oil was contaminated with molds after nine days, which indicated that encapsulation of EO was needed to maintain its effectiveness as an antifungal agent.
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    Hydrogel composites loaded with plant extract as an antibacterial and quorum sensing inhibiting wound dressing
    Baranda, Marivic M. (Division of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2018-06)
    Delayed wound healing is often caused by bacterial infection. Furthermore, the persistent inflammation brought by the formation of biofilm via quorum sensing causes pain and discomfort. In a novel effort, this study fabricated an antibacterial and quorum sensing inhibiting hydrogel dressing loaded with plant extracts. KC-PVP hydrogel composites were loaded separately with lg/mL C. longa and H. durvillei extract under optimized conditions. The hydrogels were characterized through swelling test and FTIR analysis. It was determined that C. longa-loaded and H. durvillei-loaded hydrogels have a maximum swelling capacity of 175% and 135%, respectively. The antibacterial efficacy and the quorum sensing inhibition activity of extract-loaded hydrogels were evaluated using soft-layer agar well diffusion method. Hydrogel loaded with C. longa extract displayed 47.0 (±1.0), 45.0 (±1.0), and 32.33 (±0.58) mm mean growth inhibition against S. aureus, E. coli, and P. aeruginosa, respectively. H. durvillei extract-loaded hydrogels inhibited the growth of S. aureus, E. coli, and P. aeruginosa by 45.33 (±0.58), 43.67 (±1.52), and 33.33 (±0.58) mm in diameter, respectively. The quorum-sensing inhibition zone of C. longa extract and H. durvillei extract-loaded hydrogels were 49.63 (±0.40) and 49.37 (±1.11) mm in diameter, respectively. Overall, the extract-loaded hydrogel composites exhibited good antibacterial and quorum-sensing inhibiting activity, potentially for wound treatment.
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    Phytochemical screening, antibacterial and antioxidant activities of the crude ethanolic extract of calamansi (Citrus microcarpa) peels
    Baquiano, Clarie Dean C. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2015-06)
    Citrus fruits are well-known sources of vitamin C, and like most other whole foods, they also contain an impressive list of other essential nutrients and high amounts of phytochemicals. Calamansi, scientifically known as Citrus microcarpa, is a native citrus plant cultivated throughout the Philippines. Calamansi is chiefly utilized for its pulp and juice but the peels and pressed pulp, covering each individual segment of the edible portion and seeds are considered as wastes and thrown away. To further utilize the rind or peels, this study was conducted to investigate the antioxidant and antibacterial properties of the crude ethanolic extract of C. microcarpa peels. Presence of select phytochemicals was also tested. The antioxidant and antibacterial activities were investigated using the DPPH (2,2-diphenyl-picrylhydrazyl) free radical scavenging assay and Kirby-Bauer disc diffusion susceptibility method, respectively. Results of the phytochemical screening have confirmed the presence of alkaloids, flavonoids, tannins, terpenoids, steroids and saponins in the extract. Crude ethanolic extract of C. microcarpa peels also showed activity against DPPH radicals with a half maximal inhibitory concentration (IC50) of 14.46 pL/mL, a much higher IC50 value compared to the positive control, ascorbic acid, with 8.36 pL/mL. The antibacterial activity assay of the extract showed minimal inhibition against E. coli and S. aureus.
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    Study and stream: University students' behavior on online academic learning and their motivations for binge-watching on Netflix
    Banting, Ericka Marie; Bitoca, Joyce Anne Marie; Buergo, Angelika (Division of Humanities, College of Arts and Sciences, University of the Philippines Visayas, 2022-06)
    The Covid 19 pandemic brought Netflix to unprecedented heights, especially among people aged 18 to 24. The remote setup of learning enabled students to multi-task between their schoolwork and non-academic activities. In particular, this study looks into the phenomenon of binge-watching among communication students from a selected university who are direct subscribers of Netflix. Using the framework designed by Panda and Pandey (2017) on the motivations and outcomes of binge-watching among college students, this research investigates if there is a relationship between the university students' behaviors on online academic learning and the factors that motivate them to binge-watch TV shows/series on Netflix. The research design is quantitative in nature and data analysis utilizes the Pearson's R correlation. Findings show a moderately small, positive correlation between the university students' behavior towards online academic learning and their motivations to binge-watch TV shows/series on Netflix. This means that progress in the student's behavior on online academic learning signifies an increase in their motivations for binge-watching.
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    Phytochemical screening, antioxidant activity, and comparative bioefficacy assessment of Camansi (Artocarpus camansi) seed extracts for in vitro antibacterial activity
    Bantayan, Andrea Marie B. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2016-06)
    This study was conducted to compare and assess the antibacterial and antioxidative properties, and to screen for the presence of phytochemical constituents in the different solvent-derived extracts of Artocarpus camansi seeds from Mintal, Davao City. Phytochemical analysis revealed the presence of alkaloids, flavonoids, glycosides, phenol, steroids, tannins, and terpenoids in the methanol extract. The antibacterial efficacy was determined by agar well diffusion method against B. subtilis and E. coli. Streptomycin was used as a standard drug for the study of antibacterial activity. Sensitivity in terms of zones of inhibition and phytochemical composition of the different extracts were also determined. Results showed that all extracts were effective against both of the bacteria tested with the pet. ether and DCM extracts showing moderate activity, and the methanol extract exhibiting strong activity against B. subtilis and E. coli. Furthermore, methanol extract of A. camansi seeds possessed strong DPPH radical scavenging activity. In the DPPH method, the pet. ether, DCM, and methanol extracts showed free radical inhibition of 18.96±0.16%, 21.62±0.14%, and 90.05±0.08%, respectively. The IC50 value obtained for the methanol seed extract at concentration 0.05 g/mL was 6.67±0.004 g/L. The current findings suggest that the phytochemicals present in the methanol extract of Artocarpus camansi seeds (alkaloids, flavonoids, glycosides, phenol, steroids, tannins, and terpenoids) may be responsible for the antibacterial and antioxidant activity.
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    Economic cost of dengue fever treatment in Miagao, Iloilo
    Banehit, Gracesel Faith D.; Napulan, Macie Carlyn A. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2017-06)
    Dengue, the most rapidly spreading mosquito-borne viral infection in the world, has caused many deaths in the Philippines. The municipality of Miagao, Iloilo Province, has recorded a high number of dengue cases in recent years. This study determined the economic cost of dengue fever treatment among affected households and patients with dengue in 2016 in Miagao. The study used a cross-sectional data collected from 169 interviews with the study participants for 201 dengue patients from 62 barangays in Miagao in February to April 2017. The study identified, measured, and valued the various costs related to dengue fever treatment. Results showed that the total economic cost for home-treated patients averaged at Pl2,522.08 for both the patient and the caregiver (household), with the following breakdown: P5,068.82 for monetary costs (direct medical and non-medical costs) and P7,453.26 as opportunity cost. For those who were admitted in the hospital, the total economic cost averaged at P22,213.31 with the following breakdown: P9,504.77 for monetary costs and P12,708.54 for opportunity cost. As expected, patients treated at home had lower costs both in monetary and nonmonetary aspects compared to those confined in the hospital. Although the majority of the study participants are knowledgeable about dengue, it is still necessary to increase their awareness of dengue prevention in order to prevent dengue treatment costs for the households. The local government must also strengthen the implementation of its existing dengue prevention policies and programs to mobilize cooperation and involvement of the people in dengue prevention.
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    Antioxidative properties of the fruits of selected tomato (Lycopersicon esculentum Mill.) varieties
    Baltazar, Gleza Wae D. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2016-05)
    This study was conducted to compare the antioxidative properties of greenstage fruits of selected Lycopersicon esculentum Miller varieties (Diamante, Diamante Max, and Pidada) from Barangay Durog, Miagao, Iloilo. The fresh fruit samples were extracted with 95% ethanol. The crude extracts were concentrated using rotary evaporator. The antioxidant activity of the ethanolic fruit extracts was assayed for DPPH radical scavenging activity and the IC50 values were determined. The determination of the phenolic and flavonoid contents was done employing Folin- Ciocalteu method and aluminum chloride spectrophotometric method, respectively. The results show that the fruits of the studied L. esculentum varieties exhibit DPPH radical scavenging activity. The IC50 values, however, differ significantly among the varieties. The IC50 values for Diamante (24.22 ± 1.41 g/L) and Diamante Max (25.08 ± 0.45 g/L) are comparable but are significantly lower than Pidada (29.47 ± 1.33 g/L). These findings imply that Diamante and Diamante Max varieties have stronger antioxidative capacity than the Pidada variety. Moreover, the results indicate that the antioxidative property of L. esculentum is variety-dependent. The results of the determination of the total phenolic content, expressed in mg gallic acid equivalent (GAE) per g fresh sample, are in the order of 0.16 ± 0.02 mg GAE/g for Diamante > 0.15 ± 0.02 mg GAE/g for Diamante Max and Pidada. On the other hand, the results of the determination of the flavonoid content, expressed in μg quercetin equivalent (QE) per g fresh sample, are in the order of 6.31 ± 1.27 μg QE/g for Diamante >6.21 ± 0.88 μg QE/g for Diamante Max > 5.63 ± 0.94 pg QE/g for Pidada. No significant difference in the total phenolic and flavonoid contents was found among varieties. Nonetheless, the detection of phenolics and flavonoids, known antioxidants, supports the present results on the antioxidative property of the fruits of the L. esculentum varieties studied.
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    Antioxidant property, total phenolics, manganese and iron content of humic acid obtained from Sta. Barbara, Iloilo artesian well water
    Baltazar, Lenmark Anthony M. (Division of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2016-06)
    The town of Santa Barbara in Iloilo is famous for its reddish browncolored well water due to the presence of humic acid which makes up most of the water’s natural organic matter content. Because the well water is used by the local townsfolk for drinking and other domestic activities, it is imperative that a study must be made to assess its potential health benefits and risks. In this study, the DPPH radical scavenging activity of the extracted humic acid was investigated. It was determined that the sample contains antioxidants capable of reducing DPPH with an IC5o of 51.552± 0.652 ppm. Prussian Blue Assay for total phenolic content also showed that a 76.5 ppm humic acid sample has 14.169 ± 1.151 ppm in GAE. Flame-AAS analysis showed that the humic acid sample contains iron (0.8376 ± 0.0581 ppm). The manganese content of the sample was below the detection limit of the instrument. The results suggest that the humic acid extracted from the well water contains potential antioxidants which can be exploited for various commercial and medical applications.
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    Development of a high-performance thin layer chromatography (HPTLC)-densitometric method for the estimation of quercetin and kaempferol in Euphorbia hirta (Tawa-tawa) extract
    Baladjay, Diane Monique S. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2017-06)
    A sensitive, fast, efficient, and repeatable high-performance thin-layer chromatographic (HPTLC) method has been developed for simultaneous analysis of quercetin and kaempferol from the extracts of the aerial parts of Euphorbia hirta. In the development of the HPTLC method, different extraction parameters were evaluated and quantification of quercetin and kaempferol in the extract of E.hirta was performed using a densitometric software, Quantiscan™. Among the different combinations of mobile phases used, best separation was achieved in toluene: ethyl acetate: formic acid: methanol (5: 6: 2: 1) solvent system. E.hirta was extracted using the developed extraction method which involves the use of 80% ethanol, 8 minutes of sonication and serial extraction until 3rd extraction. This determined method provides a maximum yield of the compounds at minimum time and cost. The method was also validated for linearity and precision specifically repeatability. The results show that the produced calibration is linear, as supported by the obtained correlation coefficient (r) of > 0.999 and coefficient of determination (R2) of > 0.997. Moreover, the obtained slopes of the calibration curves show that the method is most sensitive towards quercetin and least sensitive to gallic acid. The method was also found out to be repeatable and precise as the %RSD for both the inter-day and intra-day trials obtained a value less than <2%. The present method is being reported for the first time and can be used for routine quality control and quantification of quercetin and kaempferol in various plant samples, extracts, and market formulations.
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    Antioxidant property of anthocyanin from malatinta (Phyllanthus reticulatus Poir.) fruits obtained by static and microwave-assisted extraction
    Baldonasa, RB Hera Angelica R. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2015-06)
    Fresh fruits of Phyllanthus reticulatus Poir. were analyzed for anthocyanin by spectrophotometric method. The antioxidant activity using 1,1 -diphenyl-2-picrylhydrazyl (DPPH ) free radical scavenging activity (FRSA) and reducing power (RP) through FeCl3 assay were determined. The use of two extraction processes, namely, the static method and microwave-assisted extraction, using two extraction solvents, acidified methanol and aqueous ethanol (1:1) were compared. The total anthocyanin content (TAC) of P. reticulatus fruit was highest in extract using static method of extraction in acidified methanol solvent (279.94 ±17.31 mg/lOOg), comparable to levels found in plants rich in anthocyanins, (e.g., berries genotypes, grapes and red cabbages). In microwave-assisted extraction, TAC using acidified methanol was higher (278.01 ± 18.76 mg/lOOg) compared to using aqueous ethanol (210.96 ± 2.02 mg/100g). P. reticulatus fruits exhibited high antioxidant activity as revealed by its RP and DPPH FRSA results when compared to ascorbic acid. These findings highlight the usefulness of P. reticulatus as a source of anthocyanins with good antioxidant properties, which, in turn, gives importance to the undervalued and underutilized plant.