Undergraduate Theses
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Item Antifungal activities of lemongrass (Cymbopogon citratus) essential oil -B-cyclodextrin inclusion complex incorporated in commercial fish feedsBaure, Jiaren G. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2019-06)During the wet season in the Philippines, fish feeds can be contaminated with mycotoxins, affecting fish production. The addition of essential oil (EO) is one of the ways to prevent this, but it is prone to physical and chemical reactions. Thus, this study aims to encapsulate lemongrass essential oil (LGEO) in B-cyclodextrin (B-CD) to produce an inclusion complex (IC) that can be used as an additive in commercial tilapia feeds to prevent or minimize fungal contamination. The inclusion complex was made using the co-precipitation method and was characterized by Fourier Transform Infrared (FTIR) Spectroscopy. The spectrum was similar to that of citral, a major component of lemongrass essential oil that is responsible for inhibiting fungi. The antifungal activities of pure and encapsulated LGEO at varying concentrations against Aspergillus species were carried out by the Agar Well Diffusion assay. Results showed that A. flavus was more susceptible in LGEO and IC compared with A. fumigatus. The tested concentration of EO could inhibit both fungi. However, it would take 50% IC to effectively inhibit the growth of both fungi. The stability of the inclusion complex in commercial fish feeds was also evaluated. The feeds were stored in an area with the appropriate conditions that could promote the growth of molds, such as temperature (>27°C), moisture levels (14%), and relative humidity levels (>70%). The results showed that the encapsulated LGEO was effective in extending the shelf life of the feeds and was best observed at a concentration of 5 g kg-1 IC. The oil remained active for nine days, wherein about 10- 40% of the feeds with 5 g kg-1 IC were covered with molds. On the other hand, about 70-100% of the surface of the feeds with essential oil was contaminated with molds after nine days, which indicated that encapsulation of EO was needed to maintain its effectiveness as an antifungal agent.Item Critical analysis of local newspapers' designBarbon, Krisna C.; Macahilo, September Noon A. (Division of Humanities, College of Arts and Sciences, University of the Philippines Visayas, 2013-04)The study, "Critical Analysis of Local Newspaper Design", gives the idea of how local newspapers in Iloilo are designed through evaluation of the four elements of design namely headline, text, photo, cutline and the over-all layout of the front page. The duration for the evaluation occurred for a span of four weeks. The subjects for the study were the seven newspapers found in Iloilo City. As a result of the evaluation, it has been found out that in headline layouting, all seven newspapers failed to be consistent in following the line-column formula and the 5-10 words headline guideline. In text layouting, the common problems are the measurements of the legs of columns and jump stories. For photos found in the front page, most of them do not have borders and with excessive use of photo cliches. The photos all look natural but do not suggest actions. For cutline, not all newspapers observed proper placement which should be outside the photo. For the overall layout, majority of the newspapers have balance. The researchers observed that there is a need to improve the front page layout of local newspapers in Iloilo City.Item Hydrogel composites loaded with plant extract as an antibacterial and quorum sensing inhibiting wound dressingBaranda, Marivic M. (Division of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2018-06)Delayed wound healing is often caused by bacterial infection. Furthermore, the persistent inflammation brought by the formation of biofilm via quorum sensing causes pain and discomfort. In a novel effort, this study fabricated an antibacterial and quorum sensing inhibiting hydrogel dressing loaded with plant extracts. KC-PVP hydrogel composites were loaded separately with lg/mL C. longa and H. durvillei extract under optimized conditions. The hydrogels were characterized through swelling test and FTIR analysis. It was determined that C. longa-loaded and H. durvillei-loaded hydrogels have a maximum swelling capacity of 175% and 135%, respectively. The antibacterial efficacy and the quorum sensing inhibition activity of extract-loaded hydrogels were evaluated using soft-layer agar well diffusion method. Hydrogel loaded with C. longa extract displayed 47.0 (±1.0), 45.0 (±1.0), and 32.33 (±0.58) mm mean growth inhibition against S. aureus, E. coli, and P. aeruginosa, respectively. H. durvillei extract-loaded hydrogels inhibited the growth of S. aureus, E. coli, and P. aeruginosa by 45.33 (±0.58), 43.67 (±1.52), and 33.33 (±0.58) mm in diameter, respectively. The quorum-sensing inhibition zone of C. longa extract and H. durvillei extract-loaded hydrogels were 49.63 (±0.40) and 49.37 (±1.11) mm in diameter, respectively. Overall, the extract-loaded hydrogel composites exhibited good antibacterial and quorum-sensing inhibiting activity, potentially for wound treatment.Item Phytochemical screening, antibacterial and antioxidant activities of the crude ethanolic extract of calamansi (Citrus microcarpa) peelsBaquiano, Clarie Dean C. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2015-06)Citrus fruits are well-known sources of vitamin C, and like most other whole foods, they also contain an impressive list of other essential nutrients and high amounts of phytochemicals. Calamansi, scientifically known as Citrus microcarpa, is a native citrus plant cultivated throughout the Philippines. Calamansi is chiefly utilized for its pulp and juice but the peels and pressed pulp, covering each individual segment of the edible portion and seeds are considered as wastes and thrown away. To further utilize the rind or peels, this study was conducted to investigate the antioxidant and antibacterial properties of the crude ethanolic extract of C. microcarpa peels. Presence of select phytochemicals was also tested. The antioxidant and antibacterial activities were investigated using the DPPH (2,2-diphenyl-picrylhydrazyl) free radical scavenging assay and Kirby-Bauer disc diffusion susceptibility method, respectively. Results of the phytochemical screening have confirmed the presence of alkaloids, flavonoids, tannins, terpenoids, steroids and saponins in the extract. Crude ethanolic extract of C. microcarpa peels also showed activity against DPPH radicals with a half maximal inhibitory concentration (IC50) of 14.46 pL/mL, a much higher IC50 value compared to the positive control, ascorbic acid, with 8.36 pL/mL. The antibacterial activity assay of the extract showed minimal inhibition against E. coli and S. aureus.Item Phytochemical screening, antioxidant activity, and comparative bioefficacy assessment of Camansi (Artocarpus camansi) seed extracts for in vitro antibacterial activityBantayan, Andrea Marie B. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2016-06)This study was conducted to compare and assess the antibacterial and antioxidative properties, and to screen for the presence of phytochemical constituents in the different solvent-derived extracts of Artocarpus camansi seeds from Mintal, Davao City. Phytochemical analysis revealed the presence of alkaloids, flavonoids, glycosides, phenol, steroids, tannins, and terpenoids in the methanol extract. The antibacterial efficacy was determined by agar well diffusion method against B. subtilis and E. coli. Streptomycin was used as a standard drug for the study of antibacterial activity. Sensitivity in terms of zones of inhibition and phytochemical composition of the different extracts were also determined. Results showed that all extracts were effective against both of the bacteria tested with the pet. ether and DCM extracts showing moderate activity, and the methanol extract exhibiting strong activity against B. subtilis and E. coli. Furthermore, methanol extract of A. camansi seeds possessed strong DPPH radical scavenging activity. In the DPPH method, the pet. ether, DCM, and methanol extracts showed free radical inhibition of 18.96±0.16%, 21.62±0.14%, and 90.05±0.08%, respectively. The IC50 value obtained for the methanol seed extract at concentration 0.05 g/mL was 6.67±0.004 g/L. The current findings suggest that the phytochemicals present in the methanol extract of Artocarpus camansi seeds (alkaloids, flavonoids, glycosides, phenol, steroids, tannins, and terpenoids) may be responsible for the antibacterial and antioxidant activity.Item A comparison of spelling in texting and formal writingBarrientos, Shiela Rose Z.; Vencer, Ruby Jean A. (Division of Humanities, College of Arts and Sciences, University of the Philippines Visayas, 2011-03)This study, entitled A Comparison of Spelling in Texting and Formal Writing, shows the similarities and differences in spelling by high school students on mobile communication and in formal writing. It also provides educators basic information on whether or not they should worry over the spelling skills of students. And it will somehow shed light on the ongoing debate as to the perceived influence of text messaging on the writing skills of students. The respondents of the study were fourth-year students of Palaca-Damilisan National High School. Using essays and text messages written and composed by the high school students, the researchers aimed to describe how high school students construct messages using cellular phone, find out their commonly misspelled words in English language based on the set of words provided by the researchers, and finally to compare the way high school students spell words in text messaging and formal writing. Using the Theory of Planned Behavior of Ajzen (2006) that shows how a person’s beliefs, the social pressure to conform to the wishes of others, and perceived ability to carry out the action can affect his behavior such as spelling words in text messaging and formal writing. After the necessary data were gathered, the researchers found that majority of the respondents scored low in spelling in text messaging and scored average in the formal writing. Twenty or 48% has a low score both in text messaging and formal writing. Only two respondents got an average score both in text messaging and formal writing, while only one rated high in formal writing but scored low in text messaging. The researchers found out that in text messaging, five of the 15 words in the list were consistently misspelled while in formal writing, the respondents spelled properly all the words except for one, which is irresistible. In addition, respondents used contractions, numbers and symbols as substitute for letters and omitted some vowels in text messaging, while in formal writing the respondents do not substitute letters with numbers. The researchers recommend to conduct more studies on the perceived influence of technological advancement such as cellular phone on the word construction in formal writing, improve the questionnaire so as to satisfy other elements stated in the Theory of Planned Behavior, formulate survey questions that will measure the respondents’ English language skills, conduct a study that would compare the spelling skills of cellular phone and non-cellular phone users, and conduct the study on a group of respondents who are cellular phone users and have a high competence in the English language.Item Community structure of beach forest species in Mag-aba, Pandan, AntiqueBanogon, Tharrah Anne O. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2019-06)The Philippine beach forest is one of the understudied and less-popular important ecosystem that provides great number of food and economic products in the country. In fact, we lost them much earlier than the mangrove community that there is miniscule information is existing about them. There is a need for understanding the forest status and community structure due to the rising threats of natural and anthropogenic factors. The unexplored forest patch in Barangay Mag-aba, Pandan, Antique can provide additional information on beach forest diversity and abundance in the country, thus the need for this study. This study aimed to record the different species and to provide description of the community of beach forest in Mag-aba, Pandan. Specifically, the objectives of this study are (1) identify, describe and classify the beach forest species present in the area, and (2) describe the community structure in terms of species composition, diameter at breast height and stand height, stand basal area, importance value, evenness and Shannon- Weiner Index of Diversity. The transect plot method adopted from English et al (1994) was used to obtained quantitative data. A total of 80 beach forest species representing 41 families were recorded. The family Fabaceae and the species Cocos nucifera dominated the forest community. The stand basal area of 83.24 m2 ha-1 reflects a mature beach forest community, however the community is made up of mostly low DBH classes (saplings and seedling) also indicating a regenerating forest. Mag-aba is characterized to have a low species diversity (H'=1.17), however the individual species are fairly distributed (J'- 0.83). These results are addition to the existing knowledge of beach forest community that would serve as information for conservation measures.Item Economic cost of dengue fever treatment in Miagao, IloiloBanehit, Gracesel Faith D.; Napulan, Macie Carlyn A. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2017-06)Dengue, the most rapidly spreading mosquito-borne viral infection in the world, has caused many deaths in the Philippines. The municipality of Miagao, Iloilo Province, has recorded a high number of dengue cases in recent years. This study determined the economic cost of dengue fever treatment among affected households and patients with dengue in 2016 in Miagao. The study used a cross-sectional data collected from 169 interviews with the study participants for 201 dengue patients from 62 barangays in Miagao in February to April 2017. The study identified, measured, and valued the various costs related to dengue fever treatment. Results showed that the total economic cost for home-treated patients averaged at Pl2,522.08 for both the patient and the caregiver (household), with the following breakdown: P5,068.82 for monetary costs (direct medical and non-medical costs) and P7,453.26 as opportunity cost. For those who were admitted in the hospital, the total economic cost averaged at P22,213.31 with the following breakdown: P9,504.77 for monetary costs and P12,708.54 for opportunity cost. As expected, patients treated at home had lower costs both in monetary and nonmonetary aspects compared to those confined in the hospital. Although the majority of the study participants are knowledgeable about dengue, it is still necessary to increase their awareness of dengue prevention in order to prevent dengue treatment costs for the households. The local government must also strengthen the implementation of its existing dengue prevention policies and programs to mobilize cooperation and involvement of the people in dengue prevention.Item Antioxidative properties of the fruits of selected tomato (Lycopersicon esculentum Mill.) varietiesBaltazar, Gleza Wae D. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2016-05)This study was conducted to compare the antioxidative properties of greenstage fruits of selected Lycopersicon esculentum Miller varieties (Diamante, Diamante Max, and Pidada) from Barangay Durog, Miagao, Iloilo. The fresh fruit samples were extracted with 95% ethanol. The crude extracts were concentrated using rotary evaporator. The antioxidant activity of the ethanolic fruit extracts was assayed for DPPH radical scavenging activity and the IC50 values were determined. The determination of the phenolic and flavonoid contents was done employing Folin- Ciocalteu method and aluminum chloride spectrophotometric method, respectively. The results show that the fruits of the studied L. esculentum varieties exhibit DPPH radical scavenging activity. The IC50 values, however, differ significantly among the varieties. The IC50 values for Diamante (24.22 ± 1.41 g/L) and Diamante Max (25.08 ± 0.45 g/L) are comparable but are significantly lower than Pidada (29.47 ± 1.33 g/L). These findings imply that Diamante and Diamante Max varieties have stronger antioxidative capacity than the Pidada variety. Moreover, the results indicate that the antioxidative property of L. esculentum is variety-dependent. The results of the determination of the total phenolic content, expressed in mg gallic acid equivalent (GAE) per g fresh sample, are in the order of 0.16 ± 0.02 mg GAE/g for Diamante > 0.15 ± 0.02 mg GAE/g for Diamante Max and Pidada. On the other hand, the results of the determination of the flavonoid content, expressed in μg quercetin equivalent (QE) per g fresh sample, are in the order of 6.31 ± 1.27 μg QE/g for Diamante >6.21 ± 0.88 μg QE/g for Diamante Max > 5.63 ± 0.94 pg QE/g for Pidada. No significant difference in the total phenolic and flavonoid contents was found among varieties. Nonetheless, the detection of phenolics and flavonoids, known antioxidants, supports the present results on the antioxidative property of the fruits of the L. esculentum varieties studied.Item Antioxidant property, total phenolics, manganese and iron content of humic acid obtained from Sta. Barbara, Iloilo artesian well waterBaltazar, Lenmark Anthony M. (Division of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2016-06)The town of Santa Barbara in Iloilo is famous for its reddish browncolored well water due to the presence of humic acid which makes up most of the water’s natural organic matter content. Because the well water is used by the local townsfolk for drinking and other domestic activities, it is imperative that a study must be made to assess its potential health benefits and risks. In this study, the DPPH radical scavenging activity of the extracted humic acid was investigated. It was determined that the sample contains antioxidants capable of reducing DPPH with an IC5o of 51.552± 0.652 ppm. Prussian Blue Assay for total phenolic content also showed that a 76.5 ppm humic acid sample has 14.169 ± 1.151 ppm in GAE. Flame-AAS analysis showed that the humic acid sample contains iron (0.8376 ± 0.0581 ppm). The manganese content of the sample was below the detection limit of the instrument. The results suggest that the humic acid extracted from the well water contains potential antioxidants which can be exploited for various commercial and medical applications.