Undergraduate Theses
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Item The pharmacological screening of Moringa oleifera Lam.Burgos, Karen J. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1998-07)Moringa oleifera, Lam, or malunggay, belongs to Family Moringaceae. The medicinal value of this plant has long been recognized in folk medicine. Not all the claims, however, are proven scientifically. This study aimed to (1) test the crude extracts from the seeds and leaves of M. oleifera for pharmacological properties and (2) to compare its efficacy with the drugs commonly used to alleviate particular ailment. Phytochemical assay for alkaloid using Culvenor-Fitzgerald method was done to test the presence of alkaloids. The dried seeds and young leaves were extracted with methanol and concentrated under reduced pressure at 45°C. The resulting extracts were partitioned and used for the different assays: Brine shrimp assay for cytotoxicity, Acetic acid-induced writhing assay for analgesic activity, Charcoal tracing assay for antispasmodic activity and paper disc diffusion method for antimicrobial activity. Result showed that only the seeds have alkaloids. Toxicity test revealed that the seeds are practically non-toxic but the leaves were toxic with an LC50 of 550 μg/mL after 24 hours of exposure. Of the two extracts, only the leaves exhibited analgesic activity although not as effective as Mefenamic Acid in reducing pain. The leaf extract, was also found active against Bacillus subtilis, Staphylococcus aureus , Escherichia coli,and Pseudomonas aeruginosa. The seed extract was active only against B. subtilis. Both extracts, however, are not as effective as the antibiotic drug, Amoxicillin. Results also showed that both extracts do not have antispasmodic activity. The study confirmed the antimicrobial and analgesic activities for the use of the leaves of this plant in folk medicine.Item Meiofaunal assemblages in the mangrove habitat in Taklong Island, Nueva Valencia, GuimarasBocoran, Mary Grace G. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1999-03)Meiofauna are metazoans that can pass through a sieve with a mesh of 0.5mm but retained in a mesh of 0.063mm sieve. These organism are interesting to benthic scientist due to its capacity to determine the extent of pollution in the area and its role in the food web of an ecosystem. This study aims (1) to come up with a list of meiofaunal species present in the mangrove substratum, (2) to determine its density and abundance, and (3) to describe the meiofaunal environment in terms of temperature, salinity , pH, and oxygen content. The study was conducted in the mangrove habitat in Taklong Island Guimaras in February 1997. Thirty core samples were collected with a modified PVC core ( diameter=2.0 cm, length=15cm) from 3 areas: seaward, middle, and landward portions of the mangrove. There were eight broad groupings of meiofauna considered: nematodes, copepods, malacostracans, polychaetes, gastrotrichs, kinorhynchs, sipunculids, and others (including forams). The nematodes, of which there were 2 species observed, were the most abundant and comprised 42.14%. The copepods had 3 species observed and comprised 31.05%, 2 species of polychaetes comprised 5.04%,2species of gastrotrychs comprised 3.86% and malacostracans were 6.66%, kinorhynchs were 3.26%, and sipunculids were 0.89%. Unidentified organism which included foraminiferans, were 8.01 %. Range of meiofaunal density (number of individuals per cm'3 ) in three transects were 0.0042-0.1549 in the seaward area,0.0085-0.0785 in the middle area and 0.0021-0.0679 in the landward area. The nematodes had the highest density, and the sipunculids had the lowest density from the seaward to the landward area.Item Comparison of direct microscopy and concentration techniques using household bleach for the detection of acid-fast bacilli in sputumBernabe, John Marvin S.; Biaoco, Beverly G.; Castillanes, Melvic A.; Fernandez, Ia Jean F.; Geraldoy, Ruffa V.; Molina, Ryge Albert D.; Pluma, Zyra April D.; Que, Agnes Karen B.; Servidad, Mercy Jeane C.; Tamayo, Kyria Mae Marre P. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2008-03-29)Several published studies have demonstrated that the sedimentation and centrifugation concentration techniques which utilize sodium hypochlorite are more efficient in detecting acid fast bacilli in sputum smears compared to the conventional direct smear method. This study was conducted to (1) compare the proportion of AFB-positive samples by direct microscopy with the household bleach centrifugation concentration technique; (2) compare the proportion of AFB-positive samples by direct microscopy with the household bleach sedimentation concentration technique;(3) compare the proportion of AFB-positive samples by household bleach sedimentation with the centrifugation concentration techniques; and (4) compare the cost of the direct smear method, centrifugation concentration technique, and sedimentation concentration technique for detection of AFB. Sputum specimens were collected from TB-DOTS clinics in Miag-ao and San Joaquin, Iloilo and from Medicus laboratories in Iloilo City. The specimens were then processed through the direct smear method, centrifugation concentration method, and sedimentation concentration method. Out of 174 sputum samples included in this study, results revealed that (1) there was no significant difference in the proportions of AFB-positive slides detected using the direct microscopy and centrifugation concentration technique; (2) there was no significant difference in the proportions of AFB-positive slides detected using the direct microscopy and sedimentation concentration technique; (3) there was no significant difference in the proportions of AFB-positive slides detected using the centrifugation and the sedimentation concentration techniques; and (4) simple cost analysis of the three methods suggests that the direct smear method is the most cost efficient. The findings indicate that whichever method is used would yield almost the same proportions of AFB-positive slides. Internal quality control should be maintained throughout the laboratory work to obtain reliable results, as it is the manner of processing that determines the outcome of this study and its validity.Item Karyotype analysis of Kutsay Allium odoratum L. (Alliales: Alliaceae)Bermejo, Jo-am Asis (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1995-05)The C-metaphase count of Allium odoratum revealed that the chromosome number was 2n=32, consisting of the following centromeric groups based on Levan's method of chromosome classification: Group I chromosomes with subterminal centromeres (1 pair); Group II with submedian centromeres (2 pairs), and Group III with median centromeres (13 pairs). The mean total lengths of chromosome ranged from 8.534±0.504-16.624±0.600 μ. Mitotic index for the species was found to be 10.87%.Item Agrarian reform implementation in Barangay Indag-an, Miag-ao, IloiloBeñosa, Zoraida P. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1996-03)Agrarian Reform is a program set by the government to correct socio-economic imbalances by distributing lands to the farmers who are landless It is a means of uplifting the farmers from social inequalities and the bondage of poverty. Given the nature of the program, it is therefore important to assess the effectiveness of Agrarian Reform Implementation in the community level. Specifically, this is a study of Agrarian Reform Implementation in barangay Indag-an, Miag-ao, Iloilo. The objectives of the study are to describe the processes involved in the implementation of Agrarian Reform in Indag-an, to identify the benefits and support services extended by the program to its beneficiaries, to assess the effectiveness particularly the strengths and weaknesses of the program as perceived by its beneficiaries, to describe the relative effects on male and female household members and lastly, to draw implications on how to improve the Agrarian Reform implementation in the community level. Given these objectives, data were gathered from 35 respondents through a focus group discussion method. Personal interview and a case study were also done to assess the effectiveness of the program. The findings revealed that some beneficiaries believed that they benefitted from the program. Others, however, were a bit cynical towards its implementation. They claimed that the program was biased in Its implementation wherein it caters to the demands of the landlords disregarding the needs and problems of the farmers concerning the distribution of lands. The study further revealed that the support services extended by the program to farmer-beneficiaries centered mainly on agricultural extension, agricultural credit, education and trainings, cooperatives and legal services. Other support services such as infrastructures, health assistance, potable water supply and irrigation system were not provided by the program. As to the strengths and weaknesses of the program, it has been found out that the farmers repressed an ambivalent view about its implementation. They perceived the program as both having strengths and weaknesses wherein the former focused mainly on the processes involved in the distribution of lands while the latter focused more on the insufficient support services provided by the program. Finally, the study revealed that majority of the beneficiaries was male and women beneficiaries comprise only a small number. The male beneficiaries dominated the farm works, relegating women to household chores and aspects of farm work such as planting, weeding, preparing and bringing the food to the fields. However, women were given the opportunity as men in giving opinions, airing their views in the organization. Generally, both had gained from the program, where each has his/her role in farm production, even though the extent of their benefits variesItem Community structure of riverine mangrove stand in Brgy. Talokgangan, Municipality of Banate, Iloilo ProvinceBelaño, Keith Dominador L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2001-04)The community structure of riverine mangrove stand in Brgy. Talokgangan, Municipality of Banate, Iloilo was studied. Ten “true” mangrove species representing seven families were encountered in the “mangals” of Balandra creek community in Brgy. Talokgangan. They were Avicennia marina, Avicennia lanata and Avicennia alba representing Family Avicenniaceae; Rhizophora apiculata and Rhizophora mucronata under Family Rhizophoraceae; Sonneratia alba of the Family Sonneratiaceae; Excoecaria agallocha under Family Euphorbiaceae; Camptostemon philippinense of the Family Bombacaceae; one from the Family Meliaceae namely Xylocarpus granatum, and Family Palmae represented by Nypa fruticans. Stand Basal Area was computed highest in Station 1 with 10.22 m2/ha. Station 1 was also the densest among the four stations with a density value of 2533 trees/ha. In terms of data evaluated per zone, the landward zone has a Stand Basal Area of 10.87 m2/ha which is slightly higher than the middle and riverward zones. The middle zone, however, was found to be the densest with 1850 trees/ha. The most dominant station was Station 1 with the highest Index of Dominance of 0.3319, however, was found to be the least diverse station. The highest Index of Diversity was computed in Stations 2 and 3, with diversity indices of 0.8976 and 0.8989 respectively. Avicennia marina was found common in all stations and the most dominant, making it the most important mangrove species. They were found to occupy the whole site, growing abundantly in wide range of conditions from areas frequently inundated by tidal flow in Station 1 to the firmer muddy substratum in the back mangrove areas. Rhizophora apiculata and Rhizophora mucronata was observed to occupy waterlogged soil conditions. Excoecaria agaliocha tend to flourish in firmer muddy substratum in open sites, whereas, Xylocarpus granatum was observed to occupy zones where salinity is lowest in areas rarely affected by tidal flow. Sonneratia alba and Camptostemon philippinense tend to grow in zones directly inundated by tidal flow.Item The effect of seaweed (Kappaphycus alvarezii) as a partial substitute for commercial feed on the growth of chickenBedia, Alma Mae S. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-08)The study determined whether the use of seaweed (Kappaphycus aIvarezii) in different amounts as a partial substitute to commercial feeds would enhance the growth of chickens, reduce feed consumption, increase feed efficiency, and increase profit. The chickens were first fed with commercial broiler starter mash feeds for 15 days. Then, on the 16th day to the 35th day, the chickens were fed with different combinations in 1:1, 1:2, and 1:3 ratios of commercial broiler finisher feeds to K. alvarezii. Chickens fed with purely commercial broiler finisher feeds served as the control. Feed consumption, liveweight gain, and feed efficiency were computed every three days, while Profitability was determined at the end of the experimental period. Results showed that the use of K. alvarezii as a partial substitute to commercial broiler finisher feeds in a 1: 1 ratio increased the profit as well as the growth of the chickens, and it is comparable to that in the control (fed with purely commercial broiler finisher feeds). When the amount of K. alvarezii in the feed ration is more than 50% (1:1 ratio), it was observed that it would hamper the growth of the broilers.Item The agricultural education outreach project in Aklan: Barangay Sibalew, BangaBautista, Reynold R. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1992-07)This study uses the concept of agricultural extension as a catalyst of change for rural development. Suffice it to say, the objective of agricultural extension is to provide the rural people the appropriate information, technology and values to make them self-reliant in the context of their own environment. However, the realisation of this goal is dependent on the extent of acceptance and participation of the rural people themselves. In this study, the Aklan Agricultural College-Agricultural Education Outreach Project in Aklan is supposed to bring self- reliance among the farmers of barangay Sibalew, Bangs by effective transfer of appropriate technology. Based on the principles of extension service, it has been determined that the A.E.0.p project in Sibalew, particularly its economic and social services, has been instrumental in improving the productivity and income and broadly, the social and economic aspects or the farmers life. Needless to say, the A.E.0.P-introduced technologies have been embraced by the majority of farmers in the barangay--which exemplifies the effectiveness of the A.E.O.P project.Item The role of key actors in the promotion of local community development: The case of KALAHI-CIDSS projects in Janiuay, IloiloBartolome, Carie Fel M.; Sotrina, Emalyn S. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2008-03)This research is centred on the research problem - what are the roles of the key actors in the promotion of local community development in the case of the Kapit-bisig Laban Sa Kahirapan-Comprehensive and Integrated Delivery of Social Services (Kalahi-Cidss) projects in the municipality of Janiuay. Iloilo. Firstly, there is a need to explain local community development in the Kalahi-Cidss projects in Janiuay. Iloilo. Next, the paper proceeds to describe the resources that are being employed in Kalahi-Cidss in Janiuay in order to meet its goals. Then, the paper directs on the description of the roles of the key actors in the Kalahi-Cidss projects in Janiuay, Iloilo. The last objective of this paper is to describe the developmental outcomes brought by the projects to the people of Janiuay. The research problem and objectives of the paper were addressed through desk research, secondary material review, project site inspection, informal interviews with the barangay residents, and key informant interview with the Kalahi-Area Coordinator in Janiuay. The method of triangulation was used to avail more complete and more reliable data with less validity threats. It was found out that the Kalahi-Cidss is the flagship program of the Philippine government in addressing poverty in the country. Its foundation lies on the understanding that when people are empowered, they have the capability to spare themselves from poverty. Resources being employed in order to meet the goals of the project were categorized into proximate and non-proximate interventions. Three barangays in Janiuay were studied. The roles of the key actors in each barangay were described in the paper. The roles of the key actors in the three barangays were compared with each other and assessed in relation to the success of the Kalahi-Cidss projects in each respective community. Key actors involved in the projects were the barangay officials and the local community. The project success and developmental outcomes depended largely on how these two actors collaborated with each other. However, most essential in the actual process was how the former performed their task as leaders of the community. Barangay officials are the initiators of local community development through the Kalahi-Cidss.Item Isolation and partial characterization of globulin from Cassava (Manihot esculenta Crantz) tubersBarbosa, May R. (Division of Physical Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2007-04)Soluble globulin was isolated using an extraction buffer (0.4 M NaCl in 35 mM potassium phosphate buffer, pH 7.6 with 0.02% Na azide). The isolated globulin was subjected to solubility tests with different NaCl concentrations. It has the highest solubility in 1.25M NaCl (1.09%), but no significant differences existed among the NaCl concentrations by analysis using one-way ANOVA at a = 0.05. Three major bands existed at LOOM NaCl dissolved globulin with molecular weight ranges of 28-33kDa, 38- 43kDa, and 65-70kDa relative to BSA. Two of these bands (38-43kDa and 65-70kDa) were observed in 0.50M and 0.75M NaCl soluble globulin. The 1.25M and 1.50M NaCl concentrations gave a single band (28-33kDa) each, while no clear bands were observed in other NaCl concentrations. Amino acid analysis revealed glutamic acid (12.09%) as the most abundant amino acid component of cassava globulin. Nine (9) essential amino acids were present. Of these nine, lysine (10.50%) is the most predominant. Cystine (0.39%) has the lowest percentage, followed by methionine (1.76%).