Undergraduate Theses
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Item Comparison of direct microscopy and concentration techniques using household bleach for the detection of acid-fast bacilli in sputumBernabe, John Marvin S.; Biaoco, Beverly G.; Castillanes, Melvic A.; Fernandez, Ia Jean F.; Geraldoy, Ruffa V.; Molina, Ryge Albert D.; Pluma, Zyra April D.; Que, Agnes Karen B.; Servidad, Mercy Jeane C.; Tamayo, Kyria Mae Marre P. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2008-03-29)Several published studies have demonstrated that the sedimentation and centrifugation concentration techniques which utilize sodium hypochlorite are more efficient in detecting acid fast bacilli in sputum smears compared to the conventional direct smear method. This study was conducted to (1) compare the proportion of AFB-positive samples by direct microscopy with the household bleach centrifugation concentration technique; (2) compare the proportion of AFB-positive samples by direct microscopy with the household bleach sedimentation concentration technique;(3) compare the proportion of AFB-positive samples by household bleach sedimentation with the centrifugation concentration techniques; and (4) compare the cost of the direct smear method, centrifugation concentration technique, and sedimentation concentration technique for detection of AFB. Sputum specimens were collected from TB-DOTS clinics in Miag-ao and San Joaquin, Iloilo and from Medicus laboratories in Iloilo City. The specimens were then processed through the direct smear method, centrifugation concentration method, and sedimentation concentration method. Out of 174 sputum samples included in this study, results revealed that (1) there was no significant difference in the proportions of AFB-positive slides detected using the direct microscopy and centrifugation concentration technique; (2) there was no significant difference in the proportions of AFB-positive slides detected using the direct microscopy and sedimentation concentration technique; (3) there was no significant difference in the proportions of AFB-positive slides detected using the centrifugation and the sedimentation concentration techniques; and (4) simple cost analysis of the three methods suggests that the direct smear method is the most cost efficient. The findings indicate that whichever method is used would yield almost the same proportions of AFB-positive slides. Internal quality control should be maintained throughout the laboratory work to obtain reliable results, as it is the manner of processing that determines the outcome of this study and its validity.Item Gender analysis on the modes and extent of participation in Community-based Fishery Resource Management (CFRM) project in Malalison island, Culasi, AntiqueBenliro, Patriana Z P.; Eraste, Concepcion E. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2003-03)A Gender Analysis on the Modes and Extent of Community Participation on CFRM in Malalison Island, Culasi, Antique was conducted in June 2002-Feruary 3003. The study aimed to determine the modes and extent of participation in Community- based Coastal Resource Management through the Community-based Fishery Resource Management (CFRM) Project using Program Monitoring with interview and focused group discussion. The difference on the modes and extent of participation of sex and membership in the association was also assessed. The study found out that male and female modes of participation in CFRM had no significant difference. The difference was in the extent of their participation, which was caused by the social roles they played. On the other hand membership in the organization was found to be a significant variable in analysing the modes and extent of participation.Item Social responsibility on the use of media technologyBeloya, Marian Kristina C.; Camancho, Rea Micah C.; Perez, Mary Lydannie V.; Villanueva, Ma. Emilie M. (Division of Humanities, College of Arts and Sciences, University of the Philippines Visayas, 2005-10-21)This study is about responsibility on the use of media technology specifically cellular phones. The study is done through a survey measuring on respondents’ behavior and awareness of social responsibility pertaining to the use of cellular phone. Residents of UPV Miagao dormitories (Balay Apitong, Balay Gumamela, Balay Lampirong, Balay Kanlaon, and Balay Madyaas) were chosen as respondents to the study. The results of the study show that the respondents are Aware of their social responsibility but they are Sometimes responsible in using their cellular phone and as senders and receivers. Therefore, the UPV Miagao dormitory residents are Quite Socially Responsible on the use of media technology specifically cellular phones.Item Community structure of riverine mangrove stand in Brgy. Talokgangan, Municipality of Banate, Iloilo ProvinceBelaño, Keith Dominador L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2001-04)The community structure of riverine mangrove stand in Brgy. Talokgangan, Municipality of Banate, Iloilo was studied. Ten “true” mangrove species representing seven families were encountered in the “mangals” of Balandra creek community in Brgy. Talokgangan. They were Avicennia marina, Avicennia lanata and Avicennia alba representing Family Avicenniaceae; Rhizophora apiculata and Rhizophora mucronata under Family Rhizophoraceae; Sonneratia alba of the Family Sonneratiaceae; Excoecaria agallocha under Family Euphorbiaceae; Camptostemon philippinense of the Family Bombacaceae; one from the Family Meliaceae namely Xylocarpus granatum, and Family Palmae represented by Nypa fruticans. Stand Basal Area was computed highest in Station 1 with 10.22 m2/ha. Station 1 was also the densest among the four stations with a density value of 2533 trees/ha. In terms of data evaluated per zone, the landward zone has a Stand Basal Area of 10.87 m2/ha which is slightly higher than the middle and riverward zones. The middle zone, however, was found to be the densest with 1850 trees/ha. The most dominant station was Station 1 with the highest Index of Dominance of 0.3319, however, was found to be the least diverse station. The highest Index of Diversity was computed in Stations 2 and 3, with diversity indices of 0.8976 and 0.8989 respectively. Avicennia marina was found common in all stations and the most dominant, making it the most important mangrove species. They were found to occupy the whole site, growing abundantly in wide range of conditions from areas frequently inundated by tidal flow in Station 1 to the firmer muddy substratum in the back mangrove areas. Rhizophora apiculata and Rhizophora mucronata was observed to occupy waterlogged soil conditions. Excoecaria agaliocha tend to flourish in firmer muddy substratum in open sites, whereas, Xylocarpus granatum was observed to occupy zones where salinity is lowest in areas rarely affected by tidal flow. Sonneratia alba and Camptostemon philippinense tend to grow in zones directly inundated by tidal flow.Item Moss (Brachythecium sp.) and a filamentous green algae (Oedogonium sp.) as bunker oil sorbentsBautista, Norwell Brian C. (Division of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Visayas, 2007-04)Oil spills are very catastrophic and very destructive events. One way of controlling the dispersion of oil from these spills is by the use of sorbents. The procurement of commercial sorbents can be very costly. Thus, this study analyzed and evaluated the efficiency and effectivity of moss (Brachythecium sp.) and a green filamentous algae Oedogonium sp. as substitutes for commercial sorbents. Brachythecium sp. can grow in any moisture—rich environment and Oedogonium sp. algae grow freely in fish ponds and in any body of stagnant, oxygen-poor water. Thus, both samples are very abundant in the environment. Sorption capacity results showed that the moss Brachythecium sp. and the algae Oedogonium sp. can sorb five times and seven times its weight, respectively. In comparison, Peat Sorb, a commercial sorbent, was recorded to sorb 15 times its weight (Emedco, 2007). The minimum sorption capacity of the moss and algae samples was recorded to be approximately equal to their weight. The adsorption isotherms of both samples follow the Langmuir plot for monolayer adsorption. In the hexane capillary rise experiment, hexane rose significantly in ground moss (72.25 mm in 7 min.) and unground moss (64.80 mm in 7 min.) compared with unground algae (23.52 mm in 7 min.) and (20.70 mm in 7 min.), indicating that moss is more hydrophobic. In water, capillary rise, on the other hand, unground algae is a better medium (5.76 mm in 4 min) compared to the rest (ground algae - 4 mm, unground moss - 5.29 mm, and ground moss — 4.11 mm, all in 4 min.). Results also revealed that the moss Brachythecium sp. and the algae Oedogonium sp. expressed hydrophobic characters in the hexane-water system by remaining in the hexane layer after agitation. The moisture content of the algae (14.85%) is twice that of moss (7.94%). This gives the moss an advantage in oil sorption. The lipid contents of algae (15.58%) and moss (11.47%) do not comprise the majority of their biomass, but the lipids aid in increasing the samples’ affinity for non-polar substances. The samples may not sorb as effectively as the commercial sorbents, but they are abundant in the environment, thus, a cheaper alternative. Like Peat Sorb, which was processed from the biomass of Sphagnum sp. moss, the samples may undergo the same process that increases the hydrophobicity several-fold and gives oil-biodegradation capabilities.Item The relationship between selected social factors and adolescent pregnancy in Iloilo CityBautista, Joseph Raymund I.; Funclara, Melinda Tordesillas (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2004-03)This study attempts to ascertain if there is a relationship between selected social factors and adolescent pregnancy. Specifically, it aims to find out if school engagement, parental supervision, foster and kinship care, religiosity, contraceptive knowledge, and mass media exposure is related to adolescent pregnancy. The researchers hypothesized that there is a relationship between these selected factors and adolescent pregnancy. Adolescent mothers between 14 to 21 years old and were either pregnant or had given birth before December 2003 from three barangays in Iloilo City were interviewed. The gathered data were then subjected to Yates corrected chi-square test of independence to find out if there is any relationship between the dependent and independent variables. It was found out that school engagement, parental supervision, level of religiosity, exposure to mass media, and knowledge in contraceptive use have no relationship with adolescent pregnancy. On the other hand, foster and kinship care was found to have a relationship with adolescent pregnancy. For future research directions, the researchers recommend to those who are interested in adolescent pregnancy to explore the interrelationship between the factors that contribute to adolescent pregnancy. The researchers further suggest that other factors, such as economic status, be explored. Increasing the number of respondents might also be helpful. Finally, the possibility that staying away from compromising situations with the opposite sex is an effective factor to prevent adolescent pregnancy could be an interesting topic.Item The role of key actors in the promotion of local community development: The case of KALAHI-CIDSS projects in Janiuay, IloiloBartolome, Carie Fel M.; Sotrina, Emalyn S. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2008-03)This research is centred on the research problem - what are the roles of the key actors in the promotion of local community development in the case of the Kapit-bisig Laban Sa Kahirapan-Comprehensive and Integrated Delivery of Social Services (Kalahi-Cidss) projects in the municipality of Janiuay. Iloilo. Firstly, there is a need to explain local community development in the Kalahi-Cidss projects in Janiuay. Iloilo. Next, the paper proceeds to describe the resources that are being employed in Kalahi-Cidss in Janiuay in order to meet its goals. Then, the paper directs on the description of the roles of the key actors in the Kalahi-Cidss projects in Janiuay, Iloilo. The last objective of this paper is to describe the developmental outcomes brought by the projects to the people of Janiuay. The research problem and objectives of the paper were addressed through desk research, secondary material review, project site inspection, informal interviews with the barangay residents, and key informant interview with the Kalahi-Area Coordinator in Janiuay. The method of triangulation was used to avail more complete and more reliable data with less validity threats. It was found out that the Kalahi-Cidss is the flagship program of the Philippine government in addressing poverty in the country. Its foundation lies on the understanding that when people are empowered, they have the capability to spare themselves from poverty. Resources being employed in order to meet the goals of the project were categorized into proximate and non-proximate interventions. Three barangays in Janiuay were studied. The roles of the key actors in each barangay were described in the paper. The roles of the key actors in the three barangays were compared with each other and assessed in relation to the success of the Kalahi-Cidss projects in each respective community. Key actors involved in the projects were the barangay officials and the local community. The project success and developmental outcomes depended largely on how these two actors collaborated with each other. However, most essential in the actual process was how the former performed their task as leaders of the community. Barangay officials are the initiators of local community development through the Kalahi-Cidss.Item Isolation and partial characterization of globulin from Cassava (Manihot esculenta Crantz) tubersBarbosa, May R. (Division of Physical Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2007-04)Soluble globulin was isolated using an extraction buffer (0.4 M NaCl in 35 mM potassium phosphate buffer, pH 7.6 with 0.02% Na azide). The isolated globulin was subjected to solubility tests with different NaCl concentrations. It has the highest solubility in 1.25M NaCl (1.09%), but no significant differences existed among the NaCl concentrations by analysis using one-way ANOVA at a = 0.05. Three major bands existed at LOOM NaCl dissolved globulin with molecular weight ranges of 28-33kDa, 38- 43kDa, and 65-70kDa relative to BSA. Two of these bands (38-43kDa and 65-70kDa) were observed in 0.50M and 0.75M NaCl soluble globulin. The 1.25M and 1.50M NaCl concentrations gave a single band (28-33kDa) each, while no clear bands were observed in other NaCl concentrations. Amino acid analysis revealed glutamic acid (12.09%) as the most abundant amino acid component of cassava globulin. Nine (9) essential amino acids were present. Of these nine, lysine (10.50%) is the most predominant. Cystine (0.39%) has the lowest percentage, followed by methionine (1.76%).Item Isolation and purification and bioactive components from the fruit of Morinda citrifoliaBarbarona, Hazel M. (Division of Phyisical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Visayas, 2000-10)Phytochemical screening done on the fruit of “noni” Morinda citrifolia revealed the presence of saponins and the absence of alkaloids. Sequential extraction of active components of noni using n-hexane, dichloromethane and ethyl acetate solvents gave yields of 0.09%, 0.31% and 0.11% as yields respectively. Crude methanolic concentrate of the noni fruit was subjected into four bioactivity tests. Toxicity bioassay showed 251.19 g/ml to be the LC50. Antimicrobial bioassay showed that S. aureus, B. subtilis, E. coli, and P. auruginosa organisms have no activity against the crude methanolic concentrate. In analgesic bioassay, the crude methanolic concentrate (16.37) is no better or has the same activity with the positive control mefenamic acid (17.51) based on the percentage reduction in squirms of the Swiss Websters mice. Antispasmodic activity showed that the crude methanolic concentrate of the fruit (55.47%) is as active as the positive control, loperamide (58.02%). In the three fractions, antispasmodic activity of n-hexane and dichloromethane have greater effect than the positive control (loperamide) while ethyl acetate has the same activity with it. The percentages traveled by charcoal in n-hexane (39.929) and dichloromethane (45.908) were lower than that of the positive control, loperamide (60.130) and ethyl acetate fraction (48.352). Antispasmodic bioassay on n-hexane purified through Vacuum Liquid Chromatography showed that the nine fractions (F1-F9) showed no significant difference with the positive control. Thin Layer Chromatography showed the presence of mono-, di-, and triterpenes; steroid glycosides and saponins in the noni fruit extract.Item A content analysis of 12 episodes of the radio comedy-drama Toyang ErmitanyaBantiquete, Theola H.; Galanza, Siena Therese M.; Gersabalino, Jeniffer G. (Division of Humanities, College of Arts and Sciences, University of the Philippines Visayas, 2008-03)This study “A Content Analysis of the Radio-Comedy Drama Toyang Ermitanya” aims:(1) to identify the various issues presented in the 12 episodes of the drama (Episodes 256-267, aired in July 2003); (2) to classify the issues into different categories such as political, social, economic, religious, educational and others (gender issues); (3) to determine which of the six categories are frequently raised, and; (4) to determine whether or not the drama reflects social reality. The researchers used a sell-devised descriptive code sheet in analyzing and categorizing the issues that are presented in the drama. They set six categories where each issue would fall. Percentage distribution was used to determine which among the six categories is frequently raised. They also provided actual samples of the issues under each category, quoted in the Hiligaynon language with its corresponding translation in English. Researched on the events that happened in July 2003--the time when the 12 episodes were aired over Bombo Rady Iloilo— were then related to the issues that were presented in the 12 episodes of the drama in order to determine whether or not Toyang Ermitanya reflects social reality. This study is significant for it provides an in-depth treatment of the societal issues, thus, making people aware of the prevalent issues confronting the society at the same time. Second, it serves as an important tool in analyzing the role of media in presenting various issues of the society in a manner that almost everyone can relate to. In the process, it will guide media practitioners in producing a satirical program that will catch the interest, and at the same time, meet the need of the society in terms of information and entertainment. The researchers identified 163 issues that were raised in the 12 episodes of the drama Toyang Ermitanya. Results of the study show that social issues are frequently raised in the drama. It is followed by political, economic, religious, others (gender issues), and finally, educational which got a zero percentage. Using the Cone-effect theory, the study shows that Radio Comedy-Drama Toyang Ermitanya reflects social reality. The study would have been better if the researchers interviewed the producer/ scriptwriter of Toyang Ermitanya. For future researchers, the researchers recommend that research on another radio drama be done to further validate this study’s claim that radio dramas are not only for entertainment, but, are also a good source of information and reflect social realities.