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Undergraduate Theses

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Committee Member: search.filters.committeemember.Rojo, Josie B.Keyword: search.filters.keyword.Antibacterial activity

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    Hydrogel composites loaded with plant extract as an antibacterial and quorum sensing inhibiting wound dressing
    Baranda, Marivic M. (Division of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2018-06)
    Delayed wound healing is often caused by bacterial infection. Furthermore, the persistent inflammation brought by the formation of biofilm via quorum sensing causes pain and discomfort. In a novel effort, this study fabricated an antibacterial and quorum sensing inhibiting hydrogel dressing loaded with plant extracts. KC-PVP hydrogel composites were loaded separately with lg/mL C. longa and H. durvillei extract under optimized conditions. The hydrogels were characterized through swelling test and FTIR analysis. It was determined that C. longa-loaded and H. durvillei-loaded hydrogels have a maximum swelling capacity of 175% and 135%, respectively. The antibacterial efficacy and the quorum sensing inhibition activity of extract-loaded hydrogels were evaluated using soft-layer agar well diffusion method. Hydrogel loaded with C. longa extract displayed 47.0 (±1.0), 45.0 (±1.0), and 32.33 (±0.58) mm mean growth inhibition against S. aureus, E. coli, and P. aeruginosa, respectively. H. durvillei extract-loaded hydrogels inhibited the growth of S. aureus, E. coli, and P. aeruginosa by 45.33 (±0.58), 43.67 (±1.52), and 33.33 (±0.58) mm in diameter, respectively. The quorum-sensing inhibition zone of C. longa extract and H. durvillei extract-loaded hydrogels were 49.63 (±0.40) and 49.37 (±1.11) mm in diameter, respectively. Overall, the extract-loaded hydrogel composites exhibited good antibacterial and quorum-sensing inhibiting activity, potentially for wound treatment.
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    A novel water-in-oil-in-water emulsion to encapsulate antibacterial agents
    Balcarcel, Angelica V. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2018-06)
    Encapsulating bioactive compounds can protect them from adverse conditions. This study aimed to encapsulate bioactive agents in a stable multiple emulsion system using a material that can be recovered easily from a product abundant here in the country. Results of the study revealed that the method of encapsulation used is effective in encapsulating and releasing the bioactive compound encapsulated. The antibacterial activity determination showed that encapsulated chlorhexidine is effective against both S. aureus and E. coli bacteria. Further investigation was made by encapsulating aqueous guava (Psidium guajava) extract in the same emulsion system (water-in-oil- in-water) using recovered coconut milk protein (CMP) as a gelling agent. The evaluation of the stability of the emulsions revealed that the treatment with 40% guava extract in the aqueous phase of its primary emulsion (Emulsion 3) is the most stable among the other treatments. This treatment showed the smallest droplet size range and most uniformed droplet sizes. The creaming index of this treatment also showed the lowest values after three (3) weeks of storage. On the other hand, the treatment with 60% guava extract in the aqueous phase of its primary emulsion (Emulsion 4) appeared to be the most stable against heating. However, no antibacterial activity was observed for any of the emulsion systems both S. aureus and E. coli bacteria. Additional test revealed that the bioactive compound present in the plant extract was disrupted by the sonication process