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Undergraduate Theses

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14583/13

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Committee Member: search.filters.committeemember.Firmo, Lucifino V.SDG: search.filters.sdg.SDG 3 - Good health and well-being

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    Comparison of direct microscopy and concentration techniques using household bleach for the detection of acid-fast bacilli in sputum
    Bernabe, John Marvin S.; Biaoco, Beverly G.; Castillanes, Melvic A.; Fernandez, Ia Jean F.; Geraldoy, Ruffa V.; Molina, Ryge Albert D.; Pluma, Zyra April D.; Que, Agnes Karen B.; Servidad, Mercy Jeane C.; Tamayo, Kyria Mae Marre P. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2008-03-29)
    Several published studies have demonstrated that the sedimentation and centrifugation concentration techniques which utilize sodium hypochlorite are more efficient in detecting acid fast bacilli in sputum smears compared to the conventional direct smear method. This study was conducted to (1) compare the proportion of AFB-positive samples by direct microscopy with the household bleach centrifugation concentration technique; (2) compare the proportion of AFB-positive samples by direct microscopy with the household bleach sedimentation concentration technique;(3) compare the proportion of AFB-positive samples by household bleach sedimentation with the centrifugation concentration techniques; and (4) compare the cost of the direct smear method, centrifugation concentration technique, and sedimentation concentration technique for detection of AFB. Sputum specimens were collected from TB-DOTS clinics in Miag-ao and San Joaquin, Iloilo and from Medicus laboratories in Iloilo City. The specimens were then processed through the direct smear method, centrifugation concentration method, and sedimentation concentration method. Out of 174 sputum samples included in this study, results revealed that (1) there was no significant difference in the proportions of AFB-positive slides detected using the direct microscopy and centrifugation concentration technique; (2) there was no significant difference in the proportions of AFB-positive slides detected using the direct microscopy and sedimentation concentration technique; (3) there was no significant difference in the proportions of AFB-positive slides detected using the centrifugation and the sedimentation concentration techniques; and (4) simple cost analysis of the three methods suggests that the direct smear method is the most cost efficient. The findings indicate that whichever method is used would yield almost the same proportions of AFB-positive slides. Internal quality control should be maintained throughout the laboratory work to obtain reliable results, as it is the manner of processing that determines the outcome of this study and its validity.
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    Risk factors and prevalence of dental caries among students at Tubungan Central Elementary School, Iloilo, Philippines
    Abalajon, Karl Vincent P.; Caoyonan, Liza Marie D.; Fernandez, Natasha Andrea; Gequinana, Apple Joy F.; Lava, Lara Cristy S.; Millado, Justine Bennette H.; Rivera, Tess Corgette A. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2011-03)
    Pulpitis has been the leading cause of morbidity among adults in Tubungan, Iloilo for the past three years. Pulpitis may develop from untreated dental caries as this make the deeper layer of the teeth more susceptible to bacterial colonization. Hence, this study aimed to determine the prevalence of and the risk factors associated with dental caries among school age children in Tubungan Central Elementary School. This study will further investigate the possible causes of the high in prevalence of dental caries, and eventually introduce means of decreasing its morbidity in the municipality. One hundred nineteen students from Grades one to six were included in the study. The dental caries status was determined using the DMFT (Decayed, Missing, and Filled Teeth) index. The dental caries status was determined using the DMFT (Decayed, Missing, and Filled Teeth) index. The nutritional status was assessed using Weight for Age (WFA), Height for Age (HFA), and Weight for Height (WFH) as parameters as parameters. The Oral Hygiene practices, socio-economic status and food intake of the respondents were determined intake of the respondents were determined through an interview schedule. The prevalence of dental caries was 75.6%, with a mean DMFT of 2.97. Grade 2 students had the highest prevalence (95%) and DMFT index (5.9) among the grade levels. The DMFT index of each grade level was found to be significantly different (p=0.000). There was no statistical significance between the DMFT indices between males and females, although the mean DMFT of females was higher than males. There is no significant association between the Socio-Economic status of the family and DMFT index of the respondents. Only food rich in cereals and starches had significant association with the DMFT index. It is recommended that the government should intensify the campaigns on Oral health alongside other forms of health education and dietary and nutrition advice.