Undergraduate Theses
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Item In vitro antibacterial activity of Hibiscus rosa sinensis leaf extracts against some pathogens associated with urinary tract infectionsCabayao, Coney B. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2011-04)The in vitro antibacterial activity of Hibiscus rosa sinensis leaf ( methanolic, dichloromethane, ethyl acetate, n-hexane, and aqueous ) extracts were studied against Gram-negative (Escherichia coli and Pseudomonas aeruginosa ) and Gram-positive ( Streptococcus faecalis) bacteria. The methanolic extract was subjected to sequential liquid-liquid extraction using solvents of varying polarity and concentrations. Out of the 5 extracts, methanolic and dichloromethane extracts exhibited the highest antibacterial activity as measured using the zone of inhibition. This was followed by ethyl acetate and n-hexane extracts. At 200 mg/ml concentration, all extracts were considered active when tested with E. coli and S. faecalis, and were partially active against P. aeruginosa. The aqueous extract was inactive towards the three bacteria. The 200 mg/ml concentration showed no significant difference when compared with the positive control, amoxicillin (12.51μg/ml). This shows that Hibiscus rosa sinensis exhibits a promising antibacterial activity. Phytochemical analysis of the active fraction- the methanolic extract- showed the presence of alkaloids, tannins, glycosides, saponins, steroids, flavonoids, terpenoids, and anthraquinones. These bioactive components could be responsible for the antibacterial activity of Hibiscus rosa sinensis leaf extracts.Item Risk factors and prevalence of dental caries among students at Tubungan Central Elementary School, Iloilo, PhilippinesAbalajon, Karl Vincent P.; Caoyonan, Liza Marie D.; Fernandez, Natasha Andrea; Gequinana, Apple Joy F.; Lava, Lara Cristy S.; Millado, Justine Bennette H.; Rivera, Tess Corgette A. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2011-03)Pulpitis has been the leading cause of morbidity among adults in Tubungan, Iloilo for the past three years. Pulpitis may develop from untreated dental caries as this make the deeper layer of the teeth more susceptible to bacterial colonization. Hence, this study aimed to determine the prevalence of and the risk factors associated with dental caries among school age children in Tubungan Central Elementary School. This study will further investigate the possible causes of the high in prevalence of dental caries, and eventually introduce means of decreasing its morbidity in the municipality. One hundred nineteen students from Grades one to six were included in the study. The dental caries status was determined using the DMFT (Decayed, Missing, and Filled Teeth) index. The dental caries status was determined using the DMFT (Decayed, Missing, and Filled Teeth) index. The nutritional status was assessed using Weight for Age (WFA), Height for Age (HFA), and Weight for Height (WFH) as parameters as parameters. The Oral Hygiene practices, socio-economic status and food intake of the respondents were determined intake of the respondents were determined through an interview schedule. The prevalence of dental caries was 75.6%, with a mean DMFT of 2.97. Grade 2 students had the highest prevalence (95%) and DMFT index (5.9) among the grade levels. The DMFT index of each grade level was found to be significantly different (p=0.000). There was no statistical significance between the DMFT indices between males and females, although the mean DMFT of females was higher than males. There is no significant association between the Socio-Economic status of the family and DMFT index of the respondents. Only food rich in cereals and starches had significant association with the DMFT index. It is recommended that the government should intensify the campaigns on Oral health alongside other forms of health education and dietary and nutrition advice.
