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Undergraduate Theses

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    Toxicity of the aqueous leaf extract of datilis (Muntingia calabura) on the fry and fingerling of Oreochromis niloticus
    Cagampang, Ma. Hazel C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-08)
    A 96-hour static bioassay on the effects datilis (Muntingia calabura) leaf extract on the fry and fingerlings of Oreochromis niloticus was conducted to determine the Average Median Lethal Concentration (LC50) of datilis (Muntingia calabura) leaf extract on the fry and fingerlings of Oreochromis niloticus and to describe the behavioral responses of the fish to the extract. The assay consisted of six treatments: 0, 12.5, 25, 50, 100, and 200 ppm concentrations of the extract. Physico-chemical conditions (e.g. temperature, pH, salinity, dissolved oxygen, ammonia, nitrite, and hardness level of water) were monitored. Results showed that the average LC50 at the 12, and 24- hour period of the extract in fingerlings (128.61 and 92.37 ppm) were significantly higher (P<0.05) than in the fry (65.45, and 60,99) respectively. Mortality was higher at higher concentrations than at lower concentrations of the extract. Highest mortality was in 200 ppm followed by 100 ppm, then 50 ppm. No death occurred in 0, 12.5 and 25 ppm concentrations of the extract. Mortality was also significantly higher (P<0.05) in fingerlings than in fry. The fish, exhibited avoidance - response reaction and loss of equilibrium. Possibly, the death and unusual behavior manifested by the fish may be due to saponins present in the extract. Physico—chemical conditions were within the tolerable range for the fish hence, these may not have contributed to the death of fish. This study provides preliminary data on the toxic effects of the aqueous leaf extract of datilis on the fry and fingerlings of ?Oreochromis niloticus.
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    Karyotype analysis of Kutsay Allium odoratum L. (Alliales: Alliaceae)
    Bermejo, Jo-am Asis (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1995-05)
    The C-metaphase count of Allium odoratum revealed that the chromosome number was 2n=32, consisting of the following centromeric groups based on Levan's method of chromosome classification: Group I chromosomes with subterminal centromeres (1 pair); Group II with submedian centromeres (2 pairs), and Group III with median centromeres (13 pairs). The mean total lengths of chromosome ranged from 8.534±0.504-16.624±0.600 μ. Mitotic index for the species was found to be 10.87%.
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    A comparative study of the microorganisms found in the vaginal flora of pill and non pill user by Pap smear
    Bayate, Aimee Sarena L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-03)
    This study was conducted to identify and classify the organisms that are present in pill users and non-pill users. It will determine if taking of pills cause changes in the microflora of pill (combined) users and will describe the profile of both pill and non-pill users. The study use 200 prepared smears of the vagina, divided equally to both pill and non-pill users. The slides were collected at Dr. Ricardo Y. Ladrido Memorial Hospital, Lambunao, Iloilo by the family planning physician with the help of the medical technologist. The profile of the patients were also gathered to provide more information about the subjects. The organisms were identified according to their basic morphological characteristic. The results indicate that bacilli which is the normal inhabitant of the vagina is present in 200 samples. Cocci is present in both groups but occure more in women who are pill users. In the parasite group, Trichomonas vaginalis is present in women who are pill user and Candida is present in both groups but occur more in women who are pill users. The test for the estimation of the difference between two proportions reveal that the z values obtained from the experiment falls between the critical region which has a value of 2.58. Such a value indicate that the prevalence of parasites and bacteria are equal among pill and non-pill users.
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    Karyotype analysis of garlic, Allium sativum L. (Alliales; Alliaceae)
    Bandiola, Ma. Florma A. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-03)
    The C-metaphase count of Allium sativum L. showed a chromosome number of 2n = 14, consisting of six long and eight short somatic chromosomes categorized into three distinct groups: ten m type chromosomes with centromeres at the median region, two sm type chromosomes with submedian centromere and two st type chromosomes with subterminal centromere, Mitotic index for the species was computed at 16.88%.
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    Sea tidal occurrence as basis for estimating the time of child deliveries
    Abello, Jose Hernany L., Jr. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1992-03)
    The study was made to investigate the reliability of using the predicted time of occurrence of high tide or low tide as a basis of estimating the time of delivery of a child. This was conceived because of the belief and practice of "hilots" which has never been proven scientifically. The data on deliveries were obtained from the Angel Salazar Memorial General Hospital (San Jose, Antique) and the Western Visayas Medical Center (Mandurriao, Iloilo City). Random sampling was used with ten deliveries chosen for each month in the respective hospitals from the total number of spontaneous deliveries for the year 1990. The time of delivery was paired with the nearest occurrence of high or low tide based on the Predicted Tide and Current Tables for 1990 published by the National Mapping and Resource Information Authority, Coast and Geodetic Surveys Department. The raw data were subjected to regression analysis using Microstat software. The linear correlation coefficients (r) obtained were 0.960 (ASMGH) and 0.949² (WVMC) while the coefficients of determination (r² ) were 0.921 (ASMGH) and 0.901 (WVMC). A strong positive correlation between the two variables was seen in both hospitals studied. The time durations before or after the occurrence of high or low tide within which a child could be expected to be delivered on the average were 1.68 hours (ASMGH) and 1.85 hours (WVMC). The study has demonstrated that the could be delivery estimated time of occurrence of high or low tide considered as basis for estimating the time of a baby.