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Undergraduate Theses

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    Karyotype analysis of Kutsay Allium odoratum L. (Alliales: Alliaceae)
    Bermejo, Jo-am Asis (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1995-05)
    The C-metaphase count of Allium odoratum revealed that the chromosome number was 2n=32, consisting of the following centromeric groups based on Levan's method of chromosome classification: Group I chromosomes with subterminal centromeres (1 pair); Group II with submedian centromeres (2 pairs), and Group III with median centromeres (13 pairs). The mean total lengths of chromosome ranged from 8.534±0.504-16.624±0.600 μ. Mitotic index for the species was found to be 10.87%.
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    Histomorphological study of the gonads of Pholas orientalis
    Beluso, Michelle O. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-04)
    A total of five "diwal", Pholas orientalis, two of which wore obtained from Hinigaran, Neg. Occ. on January 1995 and three from Lanas, Barotac Nuevo on February 1994, were examined for the histomorphological study of the gonads of the species. Among the five samples, two were males and three were females. No hermaphrodites were encountered. Macroscopical examination showed that the gonads were diffused in the visceral mass extending from the anterior to the posterior part of the animal. The gonads were arborescent in form and creamy in color. No external sexual differences were observed. Histological examination showed that male and female gonads were scattered in the visceral mass and concentrate most around the stomach. Male and female gonads consist of follicles which were lobular and embedded in the connective tissues. Three sexual maturity stages were observed among the five specimens, which are the late active, ripe, and partially spent stages. During the late active stages in both male arid female gonads, developing gametes (spermatocytes and young oocytes, respectively) are attached to the follicular wall with maturing gametes lying free in the follicular lumen. At the ripe stage, male gonads showed mature sperms free in the follicle lumen with sperm tails radiating into the center of the lumen. In females, ripe oocytes are free in the follicle lumen with some maturing oocytes still attached to the folli- cular wall by a slender stalk. Partially spent stage was observed only in females. Spent gonads showed spaces in ovarian follicles due to loss of ova through spawning (1938). Since there is a growing demand for "diwal", interest on this species had picked up lately. One of the earliest studies on P. orientalis was the study on the "diwal" fishery in Neg. Occ. conducted by Ablan (1938). The larval biology and mariculture of the angelwing clam, Cyrtopleura cos- tata, were studied by Gustafson, et al. (1991) and investigations into spawning and hatchery technique for P. orientalis are underway (Gustafson, et al., 1991). Studies on the biology of P. orientalis are few and in fact no literature regarding reproductive biology of the species was obtained. Thus, this study was carried out with the following objectives: (1) to determine the location of the gonads in the visceral mass of P. orientalis, (2) to describe the histomorphology of the gonads, and (3) to differentiate the gonadal conditions histologically
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    Karyotype analysis of garlic, Allium sativum L. (Alliales; Alliaceae)
    Bandiola, Ma. Florma A. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-03)
    The C-metaphase count of Allium sativum L. showed a chromosome number of 2n = 14, consisting of six long and eight short somatic chromosomes categorized into three distinct groups: ten m type chromosomes with centromeres at the median region, two sm type chromosomes with submedian centromere and two st type chromosomes with subterminal centromere, Mitotic index for the species was computed at 16.88%.
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    A preliminary study on the extraction of alginic acid from three species of brown algae: Sargassum polycystum, Sargassum cristaefolium and Turbinaria sp. in Taklong Is.,Guimaras
    Adelantar, Geoffrey V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1996-04)
    Three species of brown algae were harvested in Taklong Is., Guimaras. These were Sargassum polycystum, Sargassum cristaefolium, and Turbinaria sp. These brown algae were subjected to physical and chemical treatment to extract and compare the amount of their alginic acid in the form of sodium alginate. Four seaweed collections for extraction were made between August to December, 1995. Values obtained during the first three extractions gave almost similar results. S. polycystum exhibited the highest percentage yield of sodium alginate with a mean of 22.84%. It was followed by S. cristaefolium at 22.21%. Turbinaria sp. gave the lowest yield with a mean of 14.12%. The last extraction somehow showed a decrease in the percentage yield of all three samples. S. polycystum yielded just 20.00%, S. cristaefolium at 19.00% and Turbinaria sp. at just. 10.10%. Several factors may have contributed to the differences in the amount of alginic acid of the three species of brown algae. Among these are the inherent capability of the different species of brown algae to produce alginates, the thickness and sturdiness of the samples, the viscosity of the extracts, and seasonal changes.