Undergraduate Theses
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Item Karyotype analysis of Kutsay Allium odoratum L. (Alliales: Alliaceae)Bermejo, Jo-am Asis (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1995-05)The C-metaphase count of Allium odoratum revealed that the chromosome number was 2n=32, consisting of the following centromeric groups based on Levan's method of chromosome classification: Group I chromosomes with subterminal centromeres (1 pair); Group II with submedian centromeres (2 pairs), and Group III with median centromeres (13 pairs). The mean total lengths of chromosome ranged from 8.534±0.504-16.624±0.600 μ. Mitotic index for the species was found to be 10.87%.Item Histomorphological study of the gonads of Pholas orientalisBeluso, Michelle O. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-04)A total of five "diwal", Pholas orientalis, two of which wore obtained from Hinigaran, Neg. Occ. on January 1995 and three from Lanas, Barotac Nuevo on February 1994, were examined for the histomorphological study of the gonads of the species. Among the five samples, two were males and three were females. No hermaphrodites were encountered. Macroscopical examination showed that the gonads were diffused in the visceral mass extending from the anterior to the posterior part of the animal. The gonads were arborescent in form and creamy in color. No external sexual differences were observed. Histological examination showed that male and female gonads were scattered in the visceral mass and concentrate most around the stomach. Male and female gonads consist of follicles which were lobular and embedded in the connective tissues. Three sexual maturity stages were observed among the five specimens, which are the late active, ripe, and partially spent stages. During the late active stages in both male arid female gonads, developing gametes (spermatocytes and young oocytes, respectively) are attached to the follicular wall with maturing gametes lying free in the follicular lumen. At the ripe stage, male gonads showed mature sperms free in the follicle lumen with sperm tails radiating into the center of the lumen. In females, ripe oocytes are free in the follicle lumen with some maturing oocytes still attached to the folli- cular wall by a slender stalk. Partially spent stage was observed only in females. Spent gonads showed spaces in ovarian follicles due to loss of ova through spawning (1938). Since there is a growing demand for "diwal", interest on this species had picked up lately. One of the earliest studies on P. orientalis was the study on the "diwal" fishery in Neg. Occ. conducted by Ablan (1938). The larval biology and mariculture of the angelwing clam, Cyrtopleura cos- tata, were studied by Gustafson, et al. (1991) and investigations into spawning and hatchery technique for P. orientalis are underway (Gustafson, et al., 1991). Studies on the biology of P. orientalis are few and in fact no literature regarding reproductive biology of the species was obtained. Thus, this study was carried out with the following objectives: (1) to determine the location of the gonads in the visceral mass of P. orientalis, (2) to describe the histomorphology of the gonads, and (3) to differentiate the gonadal conditions histologicallyItem The effect of seaweed (Kappaphycus alvarezii) as a partial substitute for commercial feed on the growth of chickenBedia, Alma Mae S. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-08)The study determined whether the use of seaweed (Kappaphycus aIvarezii) in different amounts as a partial substitute to commercial feeds would enhance the growth of chickens, reduce feed consumption, increase feed efficiency, and increase profit. The chickens were first fed with commercial broiler starter mash feeds for 15 days. Then, on the 16th day to the 35th day, the chickens were fed with different combinations in 1:1, 1:2, and 1:3 ratios of commercial broiler finisher feeds to K. alvarezii. Chickens fed with purely commercial broiler finisher feeds served as the control. Feed consumption, liveweight gain, and feed efficiency were computed every three days, while Profitability was determined at the end of the experimental period. Results showed that the use of K. alvarezii as a partial substitute to commercial broiler finisher feeds in a 1: 1 ratio increased the profit as well as the growth of the chickens, and it is comparable to that in the control (fed with purely commercial broiler finisher feeds). When the amount of K. alvarezii in the feed ration is more than 50% (1:1 ratio), it was observed that it would hamper the growth of the broilers.Item A comparative study of the microorganisms found in the vaginal flora of pill and non pill user by Pap smearBayate, Aimee Sarena L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-03)This study was conducted to identify and classify the organisms that are present in pill users and non-pill users. It will determine if taking of pills cause changes in the microflora of pill (combined) users and will describe the profile of both pill and non-pill users. The study use 200 prepared smears of the vagina, divided equally to both pill and non-pill users. The slides were collected at Dr. Ricardo Y. Ladrido Memorial Hospital, Lambunao, Iloilo by the family planning physician with the help of the medical technologist. The profile of the patients were also gathered to provide more information about the subjects. The organisms were identified according to their basic morphological characteristic. The results indicate that bacilli which is the normal inhabitant of the vagina is present in 200 samples. Cocci is present in both groups but occure more in women who are pill users. In the parasite group, Trichomonas vaginalis is present in women who are pill user and Candida is present in both groups but occur more in women who are pill users. The test for the estimation of the difference between two proportions reveal that the z values obtained from the experiment falls between the critical region which has a value of 2.58. Such a value indicate that the prevalence of parasites and bacteria are equal among pill and non-pill users.Item Decomposition of sugarcane bagasse by TrichodermaBangilan, Ma. Irish P. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-04)Sugarcane bagasse, an end-product after extraction of the juice of the sugarcane, was composted with the aid of Trichoderma, a compost fungus activator which shortens the decomposition process of farm wastes. Decomposition process was observed through the changes in temperature and substrate consistency. An average of 94 days was noted for a complete decomposition of 900 kg bagasse. Analysis of bagasse compost gave 0.78% nitrogen, 4.94% phosphorus, in the form of P2O5 and 1.55% potassium, in the form of K 2O. It showed that composted bagasse is a good source of phosporus but poor in nitrogen and potassium. The compost, nevertheless, can also be a good soil conditioner.Item Karyotype analysis of garlic, Allium sativum L. (Alliales; Alliaceae)Bandiola, Ma. Florma A. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-03)The C-metaphase count of Allium sativum L. showed a chromosome number of 2n = 14, consisting of six long and eight short somatic chromosomes categorized into three distinct groups: ten m type chromosomes with centromeres at the median region, two sm type chromosomes with submedian centromere and two st type chromosomes with subterminal centromere, Mitotic index for the species was computed at 16.88%.Item Historical analysis of Hippeatrum puniceum (Var) Lam.Bañas, Shirlyn L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1996-03)A histochemical study Hipppeastrum puniceum, a bulbous plant of Family Amaryllidaceae, was performed to screen the plant for the presence of medically important substances that could be responsible for its curative powers. Results of the analyses showed that the leaf reacted positively to tests for presence of aldehydes, alkaloids, amygdalin, and arbutin; the roots tested positive for aldehydes, alkaloids, amygdalin, lecithin, oxalic acid, saponins, and tartaric acid. The bulb had aldehydes, alkaloids, amygdalin, arbutin, lecithin, saponins and tannins. On the other hand, the leaf, root, and bulb yielded negative results in the tests for presence of presence of citric acid, formic acid, lignin, and phytosterol.Item Determination of the fungal flora in the rearing water, eggs, larvae and post-larvae of Penaeus monodon in a large-scale hatchery systemBaes, Glanee F. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1995-03)The rearing water and homogenized. eggs, larvae, post-larvae of Penaeus monodon were taken front the large-scale hatchery system. These were counted, isolated and identified. Analysis was done by determining the mean, standard deviation and skewness of the data. The result showed that the skewness of the yeast and mold counts in the rearing water were 0.93 and 0.64, respectively 'while that in the homogenized samples were 0.92 and 1.09, respectively. These showed that the data were positively skewed, hence, fungi were greater in the early larval stages than in the later stages. The mean showed that yeast yielded greater counts as compared to the molds. Furthermore, rearing water have also higher fungal count than that of the homogenized prawns. The fungi identified were Aspergillus, Balanium, Bispora, Cladosporium and Phialomysis. In the study, the fungi identified were the normal mycoflora which did not adversely harm the prawn due to favorable hatchery conditions, there being no disease outbreak during the duration of the study.Item A taxonomic survey of epiphytes on Enhalus acoroides in Ali Cove, Taklong Island, Nueva Valencia, GuimarasAlojado, Ezra Nelson E. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1996-06)Epiphytes were collected from the blades of the seagrass Enhalus acoroides which were harvested from Ali Cove, Taklong Island, Guimaras. 20 epiphytic algae were identified with the aid of taxonomic keys. Of these, five belong to the Cyanophyceae, two to the Chlorophyceae, one to the Phaeophyceae, eight to the Rhodophyceae and four to the Bacillariophyceae. Thirteen specimens were identified to the species level and seven to the genus level only. Measurements of temperature, salinity and pH were taken at the study site during the collectionItem A preliminary study on the effect of neem (Azadirachta indica) seed extract on the reproductive potential of the female Friutfly (Drosophila melanogaster)Altea, Armee H. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-04)Eqqs, first instar larvae, and third instar larvae of fruit flies (D. melanogaster), were treated with 0%, 10%, and 15% neem seed extract. This resulted in reduced number of adults that emerged from treated embryos and larvae. Females following treatment crossed with untreated males have reduced number of progeny compared with control (untreated) females. Treatment of extract at three stages of development indicated that fertilized eggs were most affected by the extract. Females derived from treated eggs were sterile. The number of progeny produced by females treated during first instar stage were considerably reduced compared to controls. On the other hand, the number of progeny produced by females treated during third instar stage was not significantly different from controls. The results suggest that neem seed extract has active components which can reduce the reproductive potential of female fruit flies exposed to the chemicals particularly at the early stages of development.