Undergraduate Theses
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Item A preliminary study on the freezing tolerance of milkfish (Chanos chanos, Forskal) embryosBuendia, Jeremiah C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-03)Milkfish embryos of blastula and C-shaped stages were tested for cryoprotectant toxicity using DMSO and glycerol at 10%, 12% and 15% concentrations. Survival and hatching as well as the effects of freezing were observed. Glycerol was more toxic than DMSO. Freezing at 4°C resulted in the decrease of hatching, and ice crystal formation killed the embryos during the process of freezing to -80°C. C-shaped embryos had a higher survival and hatching than the blastula stage embryos in the toxicity and cold shock tests.Item The effect of different levels of salinity on the spanning of ablated mudcrab Scylla serrataBueno, Jesse L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-04)Four salinity treatments with salinity levels of 15 ppt, 25 ppt, 35 ppt and 45 ppt with six replicates per treatment were utilized to determine its effect on the spawning of sexually mature bilaterally ablated mud crab Scylla serrata. The crabs range in weight from 250 grams to 580 grams and carapace width from 12.5 cm. to 16.5 cm. The results of the study showed significant difference (P<0.05) in the number of days when spawning occurred. The shortest duration when spawning occurred was at 25 ppt salinity with a mean value of 9.5 days. This was followed by 35 ppt and 15 ppt salinities with mean values of 15.69 days and 19.83 days respectively. Highest number of days when spawning occurred was at 45 ppt salinity at 24 days. All crabs died 2-3 days after spawning.Item Embryonic development and hatching rate of grouper (Epinephelus suillus) eggs incubated at different stocking densitiesBofill, Aileen B. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1992-04)Fertilized eggs of the grouper Epinephelus suillus were obtained from natural spawning of broodstock reared in a 50—t concrete tank at the Aquaculture Department of the Southeast Asian Fisheries Development Center, Tigbauan, Iloilo. The development of eggs incubated at three different stocking densities (50, 1OO, and 150 eggs/1) were monitored. Hatching rates in the different treatments were also determined. Fertilized eggs were floating, non—adhesive, transparent, and spherical in shape with a mean diameter range of 0.83 — 0.84 mm. The yolk was colorless, transparent, and possessed a single oil globule of 0.22 mm in diameter. The eggs have developed into the 2—cell stage 45 min after stocking. Eggs incubated at 1OO eggs/1 hatched 24 h after stocking, while hatching occurred after 27 h and 5 min at 50 eggs/1. No hatching was observed at 150 eggs/1 stocking density even after 28 h post—stocking. Water temperature and salinity during the experiment were 27 ° C and IB ppt, respectively.Item Karyotype analysis of Kutsay Allium odoratum L. (Alliales: Alliaceae)Bermejo, Jo-am Asis (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1995-05)The C-metaphase count of Allium odoratum revealed that the chromosome number was 2n=32, consisting of the following centromeric groups based on Levan's method of chromosome classification: Group I chromosomes with subterminal centromeres (1 pair); Group II with submedian centromeres (2 pairs), and Group III with median centromeres (13 pairs). The mean total lengths of chromosome ranged from 8.534±0.504-16.624±0.600 μ. Mitotic index for the species was found to be 10.87%.Item Histomorphological study of the gonads of Pholas orientalisBeluso, Michelle O. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-04)A total of five "diwal", Pholas orientalis, two of which wore obtained from Hinigaran, Neg. Occ. on January 1995 and three from Lanas, Barotac Nuevo on February 1994, were examined for the histomorphological study of the gonads of the species. Among the five samples, two were males and three were females. No hermaphrodites were encountered. Macroscopical examination showed that the gonads were diffused in the visceral mass extending from the anterior to the posterior part of the animal. The gonads were arborescent in form and creamy in color. No external sexual differences were observed. Histological examination showed that male and female gonads were scattered in the visceral mass and concentrate most around the stomach. Male and female gonads consist of follicles which were lobular and embedded in the connective tissues. Three sexual maturity stages were observed among the five specimens, which are the late active, ripe, and partially spent stages. During the late active stages in both male arid female gonads, developing gametes (spermatocytes and young oocytes, respectively) are attached to the follicular wall with maturing gametes lying free in the follicular lumen. At the ripe stage, male gonads showed mature sperms free in the follicle lumen with sperm tails radiating into the center of the lumen. In females, ripe oocytes are free in the follicle lumen with some maturing oocytes still attached to the folli- cular wall by a slender stalk. Partially spent stage was observed only in females. Spent gonads showed spaces in ovarian follicles due to loss of ova through spawning (1938). Since there is a growing demand for "diwal", interest on this species had picked up lately. One of the earliest studies on P. orientalis was the study on the "diwal" fishery in Neg. Occ. conducted by Ablan (1938). The larval biology and mariculture of the angelwing clam, Cyrtopleura cos- tata, were studied by Gustafson, et al. (1991) and investigations into spawning and hatchery technique for P. orientalis are underway (Gustafson, et al., 1991). Studies on the biology of P. orientalis are few and in fact no literature regarding reproductive biology of the species was obtained. Thus, this study was carried out with the following objectives: (1) to determine the location of the gonads in the visceral mass of P. orientalis, (2) to describe the histomorphology of the gonads, and (3) to differentiate the gonadal conditions histologicallyItem The effect of seaweed (Kappaphycus alvarezii) as a partial substitute for commercial feed on the growth of chickenBedia, Alma Mae S. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-08)The study determined whether the use of seaweed (Kappaphycus aIvarezii) in different amounts as a partial substitute to commercial feeds would enhance the growth of chickens, reduce feed consumption, increase feed efficiency, and increase profit. The chickens were first fed with commercial broiler starter mash feeds for 15 days. Then, on the 16th day to the 35th day, the chickens were fed with different combinations in 1:1, 1:2, and 1:3 ratios of commercial broiler finisher feeds to K. alvarezii. Chickens fed with purely commercial broiler finisher feeds served as the control. Feed consumption, liveweight gain, and feed efficiency were computed every three days, while Profitability was determined at the end of the experimental period. Results showed that the use of K. alvarezii as a partial substitute to commercial broiler finisher feeds in a 1: 1 ratio increased the profit as well as the growth of the chickens, and it is comparable to that in the control (fed with purely commercial broiler finisher feeds). When the amount of K. alvarezii in the feed ration is more than 50% (1:1 ratio), it was observed that it would hamper the growth of the broilers.Item A comparative study of the microorganisms found in the vaginal flora of pill and non pill user by Pap smearBayate, Aimee Sarena L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-03)This study was conducted to identify and classify the organisms that are present in pill users and non-pill users. It will determine if taking of pills cause changes in the microflora of pill (combined) users and will describe the profile of both pill and non-pill users. The study use 200 prepared smears of the vagina, divided equally to both pill and non-pill users. The slides were collected at Dr. Ricardo Y. Ladrido Memorial Hospital, Lambunao, Iloilo by the family planning physician with the help of the medical technologist. The profile of the patients were also gathered to provide more information about the subjects. The organisms were identified according to their basic morphological characteristic. The results indicate that bacilli which is the normal inhabitant of the vagina is present in 200 samples. Cocci is present in both groups but occure more in women who are pill users. In the parasite group, Trichomonas vaginalis is present in women who are pill user and Candida is present in both groups but occur more in women who are pill users. The test for the estimation of the difference between two proportions reveal that the z values obtained from the experiment falls between the critical region which has a value of 2.58. Such a value indicate that the prevalence of parasites and bacteria are equal among pill and non-pill users.Item Decomposition of sugarcane bagasse by TrichodermaBangilan, Ma. Irish P. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-04)Sugarcane bagasse, an end-product after extraction of the juice of the sugarcane, was composted with the aid of Trichoderma, a compost fungus activator which shortens the decomposition process of farm wastes. Decomposition process was observed through the changes in temperature and substrate consistency. An average of 94 days was noted for a complete decomposition of 900 kg bagasse. Analysis of bagasse compost gave 0.78% nitrogen, 4.94% phosphorus, in the form of P2O5 and 1.55% potassium, in the form of K 2O. It showed that composted bagasse is a good source of phosporus but poor in nitrogen and potassium. The compost, nevertheless, can also be a good soil conditioner.Item Karyotype analysis of garlic, Allium sativum L. (Alliales; Alliaceae)Bandiola, Ma. Florma A. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-03)The C-metaphase count of Allium sativum L. showed a chromosome number of 2n = 14, consisting of six long and eight short somatic chromosomes categorized into three distinct groups: ten m type chromosomes with centromeres at the median region, two sm type chromosomes with submedian centromere and two st type chromosomes with subterminal centromere, Mitotic index for the species was computed at 16.88%.Item Historical analysis of Hippeatrum puniceum (Var) Lam.Bañas, Shirlyn L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1996-03)A histochemical study Hipppeastrum puniceum, a bulbous plant of Family Amaryllidaceae, was performed to screen the plant for the presence of medically important substances that could be responsible for its curative powers. Results of the analyses showed that the leaf reacted positively to tests for presence of aldehydes, alkaloids, amygdalin, and arbutin; the roots tested positive for aldehydes, alkaloids, amygdalin, lecithin, oxalic acid, saponins, and tartaric acid. The bulb had aldehydes, alkaloids, amygdalin, arbutin, lecithin, saponins and tannins. On the other hand, the leaf, root, and bulb yielded negative results in the tests for presence of presence of citric acid, formic acid, lignin, and phytosterol.
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