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Undergraduate Theses

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14583/13

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    Development of a high-performance thin layer chromatography (HPTLC)-densitometric method for the estimation of quercetin and kaempferol in Euphorbia hirta (Tawa-tawa) extract
    Baladjay, Diane Monique S. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2017-06)
    A sensitive, fast, efficient, and repeatable high-performance thin-layer chromatographic (HPTLC) method has been developed for simultaneous analysis of quercetin and kaempferol from the extracts of the aerial parts of Euphorbia hirta. In the development of the HPTLC method, different extraction parameters were evaluated and quantification of quercetin and kaempferol in the extract of E.hirta was performed using a densitometric software, Quantiscan™. Among the different combinations of mobile phases used, best separation was achieved in toluene: ethyl acetate: formic acid: methanol (5: 6: 2: 1) solvent system. E.hirta was extracted using the developed extraction method which involves the use of 80% ethanol, 8 minutes of sonication and serial extraction until 3rd extraction. This determined method provides a maximum yield of the compounds at minimum time and cost. The method was also validated for linearity and precision specifically repeatability. The results show that the produced calibration is linear, as supported by the obtained correlation coefficient (r) of > 0.999 and coefficient of determination (R2) of > 0.997. Moreover, the obtained slopes of the calibration curves show that the method is most sensitive towards quercetin and least sensitive to gallic acid. The method was also found out to be repeatable and precise as the %RSD for both the inter-day and intra-day trials obtained a value less than <2%. The present method is being reported for the first time and can be used for routine quality control and quantification of quercetin and kaempferol in various plant samples, extracts, and market formulations.
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    Menopausal women: Knowledge, experiences, health practices and coping strategies
    Arzaga, Mary Juliebel B.; Palma, Valerie S. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2007-03)
    This study was conducted to evaluate the knowledge and beliefs of menopausal women, experiences, health practices, and their coping strategies on menopausal stage. Fifty (50) menopausal women whose age ranges from 35 to 55 were purposively selected to participate in the study. An Open-ended questionnaire on knowledge, experiences, health practices, and coping strategies scale were developed. Results showed that menopausal women entered menopause at about 46 to 50 years of age. It is also revealed that they were knowledgeable and well-informed about the process of menopause. They relied on herbal medicine both internal and external use. During the difficult periods of menopausal, they resorted to self-help and develop a closer relationship with God.