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Undergraduate Theses

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Adviser: search.filters.advisor.Pahila, Ida G.SDG: search.filters.sdg.SDG 3 - Good health and well-being

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    A preliminary study on the average yield of crude capsaicin from common varieties of Capsicum frutescens L. at two stages (Young and mature) using three extracting solvent
    Cadornigara, Ma. Sylvia C. (Division of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Visayas, 1998)
    Crude capsaicin content of the air dried and homogenized plant material of the three varieties of Capsicum frutescens L. namely cayenne, tobasco and ‘kitikot’ were determined using diethyl ether, 99.5 % ethanol and 1.0N NaOH as extracting reagents. Statistical analysis showed no significant difference on the average percent yield of crude capsaicin on the three varieties of Capsicum frutescens L. using similar extracting solvent, but a significantly higher yield of crude capsaicin was found with 99.5% ethanol and 1.0N NaOH. Through thin layer chromatography the major components of the extracts identified could probably be capsaicinoids (Rf 15-17), carotenoids (only in 99.5% ethanol, Rf 96-97), and capsanthin (Rf 27-29) using n-hexane-acetone.
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    Isolation and partial characterization of globulin from Cassava (Manihot esculenta Crantz) tubers
    Barbosa, May R. (Division of Physical Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2007-04)
    Soluble globulin was isolated using an extraction buffer (0.4 M NaCl in 35 mM potassium phosphate buffer, pH 7.6 with 0.02% Na azide). The isolated globulin was subjected to solubility tests with different NaCl concentrations. It has the highest solubility in 1.25M NaCl (1.09%), but no significant differences existed among the NaCl concentrations by analysis using one-way ANOVA at a = 0.05. Three major bands existed at LOOM NaCl dissolved globulin with molecular weight ranges of 28-33kDa, 38- 43kDa, and 65-70kDa relative to BSA. Two of these bands (38-43kDa and 65-70kDa) were observed in 0.50M and 0.75M NaCl soluble globulin. The 1.25M and 1.50M NaCl concentrations gave a single band (28-33kDa) each, while no clear bands were observed in other NaCl concentrations. Amino acid analysis revealed glutamic acid (12.09%) as the most abundant amino acid component of cassava globulin. Nine (9) essential amino acids were present. Of these nine, lysine (10.50%) is the most predominant. Cystine (0.39%) has the lowest percentage, followed by methionine (1.76%).