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Undergraduate Theses

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14583/13

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    Antidiarrheal activity of Pterocarpus indicus bark extracts using castor oil-induced diarrhea model on mice
    Cabatea, Norberto C. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2010-04)
    This study evaluated the antidiarrheal activity of the bark of Pterocarpus indicus (Narra). The crude methanolic extract of the bark was subjected to sequential extraction using hexane, DCM, and ethyl acetate. These extracts were tested for antidiarrheal activity in vitro using the castor oil induced diarrhea model in mice employing charcoal tracing method. Thin Layer Chromatography (TLC) was used in determining the chemical constituents present in every extracts using spraying reagents to aid in the visualization of spots. Crude methanolic extract dosage 200 mg/kg body weight is the comparable dosage to the reference drug loperamide (2 mg/kg body weight). The hexane extract was the most active fraction and 200 mg/kg dosage was more effective than with the positive control loperamide (2 mg/kg). No antidiarrheal activity found in the fraction of DCM and ethyl acetate extracts. Phytochemical screening revealed positive tests for tannins, phenolics, terpenes, cardiac glycosides, saponins and reducing sugars in all fractions. Tests for flavonoids and alkaloids, however, were negative.
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    Phytochemical, antioxidant, and antibacterial analysis of essential oils from Zingiber officinale Roscoe(Ginger) and Curcuma long Linn.(Turmeric)
    Alviar, Joseph Noel G. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2015-06)
    This study was conducted to characterize and compare the phytochemistry, antibacterial and antioxidant activities of essential oils extracted from the rhizomes of Zingiber officinale Roscoe (ginger) and Curcuma longa Linn. (turmeric). Percent yields in the steam distillation of ginger and turmeric were 0.42±0.02% and 0.43± 0.02%, respectively. Qualitative phytochemical screening showed that both essential oils are positive for flavonoids, phenols, saponins, terpenoids, tannins, and quinones. Antibacterial activity was tested against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli using 100%, 75%, 50%, and 25% (v/v) essential oil in ethanol with positive control tetracycline and negative control ethanol. It was determined that C. longa did not have antibacterial activity against both bacteria while Z. officinale had “moderately inhibitory” to “strongly inhibitory” activity against S. aureus with zone of inhibition (ZOI) from 17.67±1.53 mm to 23.67±0.58 mm and “weakly inhibitory” to “moderately inhibitory” activity against E. coli (ZOI from 12.33±1.15 mm to 15.67±2.08 mm). The standard antibiotic tetracycline also showed “moderately inhibitory” activity against both S. aureus (ZOl = 19.00±0.89 mm) and E. coli (ZOI = 17.33±1.21 mm). Antioxidant activity was determined by DPPH free radical inhibition. Highest % DPPH free radical inhibition was exhibited by the standard antioxidant ascorbic acid (92.98%), followed by C. longa essential oil (88.74%), and Z. officinale (76.30%).