UPV Theses and Dissertations
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Item The effect of sex reversal treatment on the growth and survival of two strains of Nile tilapia (Oreochromis niloticus) and Mossambique tilapia (Oreochromis mossambicus) raised in brackishwaterBadillo, Susan Delos Santos (University of the Philippines Visayas, 2000-08)A study to determine the effects of sex reversal (SRT) on the growth and survival of two strains of Nile tilapia (Oreochromis niloticus) and Mossambique tilapia (Oreochromis mossambicus) was conducted in four 2m x lm x 1.5m suspended hapa nets in UPV-IA Hatchery and Biology brackishwater fishpond for 75 days. The experimental treatments and codes used in this study were the following, Treatment GGn (GIFT/normal), Treatment GGsrt (GIFT/sex reversal), Treatment MMn (Moss/normal), Treatment MMsrt (Moss/sex reversal), Treatment GEn (GIFT-Egypt/normal) and Treatment GEsrt (GIFT-Egypt/sex reversal). Juvenile fry (average weight 0.0 1g) were subjected to sex reversal treatment by feeding fish diet with methyltestosterone at 60 mg per kg of feed for three weeks in freshwater. After SRT, the fingerlings were nursed in fine mesh net (0.5 mm mesh, 2m x 1m x 1m) for 30 days until the fish attained the weight from 3.0 g to 5.3 g. They were individually tagged by fin clipping method. They were acclimated from 0 ppt to 20 ppt prior to experimental proper. Juvenile fish of 3.40-5.44 g were communally stocked at 25 fish each treatment. Juvenile fish were fed with a commercial feed at 15% of their mean body weight per day from 1-30 days and 10% per day of their mean body weight from day 31-75 days culture period. Within the normal treatments, there was no significant (P>0.05) difference in mean body weights between Treatments GGn and GEn but both are significantly higher (P<0.01) than Treatment MMn. Within the sex reversed treatments, mean body weight in Treatment GGsrt was not significantly (P>0.05) different from Treatment GEsrt, but showed high significant (P<0.01) difference with Treatment MMsrt, similar to the normal treatments. The highest average final biomass of 808.88 g was obtained in Treatment GGsrt followed by Treatment GEsrt (795.58 g) and lowest in treatment MMn (367.63 g). The biomass gain of tilapia within the normal treatments ranged from 282.6 g to 672.95 g. Treatment GGn showed no significant (P>0.05) different from Treatment GEn, but both were significantly higher from Treatment MMn. The same trend was observed within sex reversal treatments. Treatment GGn obtained the highest (589.70%) percentage biomass gain and Treatment MMn (335.01%) was the lowest within the normal treatments. In sex reversal treatment, Treatment GGsrt has no significant difference with Treatment GEsrt, but both showed a high significant difference in Treatment MMsrt. Highest specific growth rate was observed in Treatment GGn (2.62 g day-1) and lowest in Treatment MMn (1.97 g day-1). Mean condition factor (MCF) of tilapia was highest in Treatment GGn (1.60), followed by Treatment GEn (1.56) and lowest in Treatment MMsrt (1.17). Within the normal treatments, Treatment GGn and GEn showed no significant difference (P>0.05), but both were significantly higher (P<0.01) than Treatment MMn. Within the SRT treatment, all treatments showed highly significant (P<0.01) difference from each other with highest at Treatment GGsrt and lowest at Treatment MMsrt. Mean survival ranged from 95% to 100%. Highest percent male population was obtained in hormone-treated fish (95-100%) compared to normal treatments (64-71%). Sex reversal treatment did not improve growth and survival of the two strains of Nile tilapia (O. niloticus), but improved the growth of Mossambique tilapia (O. mossambicus) significantly after 75 days under the conditions this experiment was conducted.Item Effects of dietary pH on growth performance and feed utilization of nile tilapia (Oreochromis niloticus)Fabay, Ryan V. (University of the Philippines Visayas, 2020-06)ABSTRACT Two feeding trials were conducted to investigate the effects of dietary pH (pH 2.5, 3.7, 4.6, 5.7-control, pH 7.0, and pH 8.0) and dietary acid source (acetic acid, citric acid, hydrochloric acid and control-no acid) on growth rate and feed utilization efficiency of the Nile tilapia fry. In addition, attractability indices of the diets at different pH and dietary acid sources were determined. Results of the first feeding trial showed that the feed with pH adjusted to 4.6 resulted in increased growth rate and improved feed utilization efficiency in the Nile tilapia. In the second feeding trial, the use of either hydrochloric, citric or acetic acid in adjusting the pH to the optimal 4.6 did not affect the growth performance of the fish. This study demonstrated that pH and not actually the kind of acid diet promoted growth and efficiency in the Nile tilapia fry. Furthermore, this study provides new information on the use of acid in aquaculture as feed additive for tilapia fry, which can be added to fish feed literature.Item Growth performance, feed utilization and survival against stress test of juvenile Nile tilapia Oreochromis niloticus fed with lactic acid bacteria (lab) Pediococcus acidilacticiSO, April Dawn P. (University of the Philippines Visayas, 2016-06)ABSTRACT The present study evaluated the growth performance, feed utilization and survival against stress test of Nile tilapia, Oreochromis niloticus fingerlings (mean initial average body weight 0.39g ± 0.01) fed with commercial probiotics. The experimental design was randomized, with four treatments and four replicates: Treatment 1 (control) with 0g kg-1 probiotics inclusion, Treatment 2 with lg kg1 probiotics inclusion, Treatment 3 with 2g kg'1 probiotics inclusion and Treatment 4 with 4g kg'1 probiotics inclusion. Three hundred and twenty fingerlings were distributed in 16 50L containers and fed for 40 days. Differences in the mean values of total weight were found at the end of the experiment. After 40 days of feeding trial, percent weight gain (%WG), specific growth rate (SGR) and and feed conversion ratio (FCR) was significantly high (P < 0.05) in treatments with probiotics compared to probiotic-free treatments; no differences on survival rate (SR) was observed among dietary treatments. The best growth performance and feed conversion was observed in fishes fed with 4g kg'1 probiotics inclusion. Challenge was done immediately after the period of application. The stress challenge test was done to the experimental animals by exposure to oxygen deficient water or asphyxia. No significant difference (P < 0.05) on SR was observed after 3 hours exposure to asphyxia. It could be concluded that probiotics can improve growth performance and feed utilization. However, further studies are recommended to assess the effects of probiotics to fish when exposed to asphyxia.Item Quality changes in Tilapia (Oreochromis niloticus) after delayed icing and subsequent storage in iceSaluan-Abduhasan, Fathma (College of Fisheries, University of the Philippines in the Visayas, 1985-04)ABSTRACT Quality changes in tilapia (Oreochromis niloticus) iced directly on harvesting were compared to those occurring when icing was delayed. Chemical and sensory examinations showed that the shelf life of tilapia was 26 days when iced immediately. 20 days and 16 days with 4 and 8 hours delay, respectively. Fish kept at ambient temperature for 12 hours were rejected after one day in ice. These results suggest that for every hour delay in icing the effective shelf life is reduced by approximately one day up to the 8th hour delay. Total volatile nitrogen values increased with storage time. Likewise, K-value and hypoxanthine values increased as storage time increased. The initial bacterial load ranged from 103- 104 cfu/g of fish with H2S-producers count of less than 100 cfu/g of fish. At rejection, the microbial load was 108~109 cfu/g of fish with HgS- producers count of 106-107 cfu/g. The typical off odours of naturally spoiling fish have been reproduced in blocks of sterile muscle by organisms such as Alteromonas putrefaciens, Pseudomonas sp, pseudomonas maltophilia, Pseudomonas flourescens and pseudomonas stutzeri.