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UPV Theses and Dissertations

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    Survey of pathogenic bacteria in bottom sediments of Iloilo River: An assessment of water sanitary quality
    Araneta, Karla Teresa S.; Bugayong, Adrienne Marrie S.; Cabanilla, Jennifer C.; Caro, Mary Ann Gina C.; Gayatgay, Christian Era U.; Nufuar, Ernest Jan T.; Rufino Krystal Hope S. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2009-03)
    This study assessed the water sanitary quality of the Iloilo River by determining the monthly heterotophic plate count (HPC), total coliform (TC), fecal coliform (FC), and fecal streptococcus (FS) counts of the bottom sediments in five sampling sites from October 2008 to February 2009. These parameters were then correlated with the physico-chemical parameters that were additionally obtained from the river water. The presence of Salmonella spp, E. coli. Vibrio spp, and fecal Streptococcus were also determined as indicators of the pathogenic bacteria in the bottom sediments of the Iloilo River. Using the FC:FS ratio, the study further distinguished fecal contamination sources from each site as human, animal, or mixed in origin. An assessment of sediment quality was done using DENR standards for TC and Kohl and Albinger's sediment quality criteria for HPC and FC. Sediment samples were taken from the following sampling sites along the Iloilo River: 1) Forbes. 2) Dungon, 3) IBRD, 4) Carpenter's, and 5) So-oc. No significant difference in any of the physico-chemical or microbiological parameters were found among sampling stations. However, analysis and comparison of the microbiological parameters of bottom sediments in terms of months showed significant temporal variability HPC, TC, and FC alternately decrease and increase every month while FS showed a generally increasing trend. The following correlations between the microbiological and physico-chemical parameters were found. 1) temperature with HPC and FS , 2) salinity with FS count, and 3) dissolved oxygen concentration with TC and FC Counts. The mean percentage of bottom sediment samples with Salmonella ranged from 3% to 10% while more than half of samples from all sampling sites showed the presence of Vibrio spp. and S. faecalis. Samples with E.coli from all sites ranged from 3 to 14%. Fecal contamination sources varied across sampling sites. So-oc show ed mostly animal sources as its orgin of fecal contamination, while other sites had either animal, human or mixed sources. S. faecalis and E. coli exhibited multiple antibiotic resistance, a grow ing health concent in many of today's rivers. All isolates were susceptible to Gentamicin and Ciprofloxacin but resistant to Penicillin. Based on HPC and FC values the degree of loading with easily decomposable organic substance and feces among the sampling sites ranges from very little to little. TC count of the sediment samples from all the sites does not exceed the limit of 5000 CFU/ 100ml set by the DENR. Results suggest that sediments were not a significant reservoir of bacterial pollution at the time this study was conducted.
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    Heavy metal concentrations in sediments from Batan Bay and its tributaries
    Andrada, Mary Royce D. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)
    The concentration of three heavy metals (Cd, Pb, Cu) in sediments from Batan Bay and its tributaries were determined in order to assess the degree of contamination of sediments in the said area. The samples were digested following the Method 3050B of US Environmental Protection Program (EPA, 1996). The heavy metal concentrations were determined using Flame Atomic Absorption Spectrometer (FAAS). Results show that the heavy metal concentrations ranged from n.d. - 2.57 mg/kg dry weight (dw) for Cd; 16.44-143.02 mg/kg dw for Cu; and 3.18-28.53 mg/kg dw for Pb. Sediment sample collected from BTN #10 has the highest Cd concentration while sediment sample collected from BTN 052 which is a rice field in Batan, Aklan registered the highest Pb and Cu concentrations. Three sediment quality guidelines (National Oceanic and Atmospheric Administration, Canadian Sediment Quality Guideline and Hong Kong Sediment Quality Guideline) were employed to assess the metal contamination in sediments. The concentrations of Cd and Pb in all sites did not exceed the maximum permissible limits set by the three guidelines. As for Cu, BTN 052 exceeded the Upper Chemical Exceedance Level set by Hong Kong SQG. Correlation analysis showed that there is a significant correlation (p <0.01) between Pb and Cu indicating possible similar source input. Special attention must be given to BTN 052 since the levels of the studied heavy metals are high in this area. The results can be used as basis for the pollution level of sediments from Batan Bay and its tributaries. Special consideration should be given to BTN 052 since all of the test metals are significantly high in this area. It is also suggested to in identify the primary sources of metal contamination in this area.