UPV Theses and Dissertations
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Item Effect of salinity on the embryonic development of Portunus pelagicus and Charybdis feriatus eggsAlba, Kristine Margaret A. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2003-03)The effect of salinity on the embryonic development of Portunus pelagicus and Charybdis feriatus was studied. The five different salinity levels used were 15 ppt, 25 ppt, 35 ppt, 45 ppt, and control (33-35 ppt) to compare their effect on the embryonic development of both species. One thousand newly spawned crab eggs were incubated in vitro in 4-L Nalgene fiberglass containers with 3-L of water at different salinity levels. Mild aeration that allows movement of eggs within the water column was provided. There were three replicates per treatment. Embryonic development of eggs (30 eggs were taken in every salinity level) was monitored daily up to hatching. The newly hatched larvae were then collected and counted. Results showed that during the incubation period, the most advanced stage of embryonic development for Portunus pelagicus was at 33-35 ppt and for Charybdis feriatus was at 35 ppt. There was normal embryonic development and hatching at 35 ppt, 45 ppt, and control (33-35 ppt) for Portunus pelagicus and at 15 ppt, 25 ppt, 35 ppt, 45 ppt, and control (33-35 ppt) for Charybdis feriatus. Eggs that were incubated at 15 ppt and 25 ppt for Portunus pelagicus died. There was normal embryonic development in both species when incubated at 45 ppt. Hatching rate for Portunus pelagicus at control (33-35 ppt) was 34.1 %, at 35 ppt was 16.1 %, and at 45 ppt was 5.8 %. Hatching rate for Charybdis feriatus at 35 ppt was 52.5 %, at control (33-35 ppt) was 21.2 %, at 45 ppt was 12.8 %, at 25 ppt was 6.7 %, and at 15 ppt was 0.3 %. To summarize, embryo of Charybdis feriatus eggs can withstand a wider range of salinity: from a low salinity of 15 ppt to a high salinity of 45 ppt, that makes them a euryhaline species. On the other hand, Portunus pelagicus cannot support embryonic development at lower salinities of 15 and 25 ppt that makes them a stenohaline species.Item Effect of salinity on survival and growth of Scylla olivacea crabletsAndres, Eileen Beth N. (Division of Biological Sciences, College of Arts ad Sciences, University of the Philippines Visayas, 2002-04)The effect of salinity on survival and growth of Scylla olivacea crablets was investigated. Salinities of 12, 16, 20, 24 and 32 ppt were used as treatments. Survival, molt interval and growth in terms of weight, carapace length and carapace width were measured for two molts. Growth was measured three days after every molt. There was no significant difference on survival of the crablets between salinities at molt 1. At molt 2, survival was significantly higher at 32 ppt compared to survival at 12 ppt but not significantly different from survival at 24, 20 and 16 ppt. Moreover, duration of survival was significantly higher at 32 ppt than at 16 and 12 ppt but not significantly different from durations of survival at 24 and 20 ppt. On the other hand, molt interval was not significantly different at salinities of 12, 16, 20, 24 and 32 ppt from C1 to C2 and from C2 to C3. Although the highest weight, carapace length and carapace width were observed at 20 ppt at molt 1, there was no significant difference in growth of the crablets at different salinities. At molt 2, the highest weight was at 32 ppt, the highest carapace length was at 24 ppt and the highest carapace width was at 16 ppt and 32 ppt. No significant difference was found in growth of the crablets between salinities at molt 2.Item Effects of pH and salinity on growth and survival of juvenile sea cucumber Holothuria scabra (Jaeger, 1833)Abagat, Christian M. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2012-03)Sea cucumber aquaculture could provide a solution to both enhance declining wild sea cucumber populations through restocking and provide sufficient beche-de-mer product to satisfy the increasing Asian market demand. The growth and survival of hatchery-reared Holothuria scabra juveniles at different pH-salinity levels were investigated. Juveniles were exposed for 14 days in six pH-salinity levels: Trt 1 (pH 6.5 — 6.7, 26-28 %o), Trt 2 (pH 6.5 - 6.7, normal salinity), Trt 3 (pH 7.0 - 7.2, 26-28 %o), Trt 4 (pH 7.0 — 7.2, normal salinity), Trt 5 (normal pH, 26-28 %o) and Trt 6 (normal pH, normal salinity) as the control. Multivariate analysis of variance (MANOVA) with repeated measurements revealed that pH and salinity had a significant effect on growth (length, width, and weight) and survival of H. scabra juveniles. Slight seawater desalination combined with high acidification (pH: 6.5-6.7) had lethal effects on H. scabra juveniles. While H. scabra juveniles could be reared in seawater with pH ≥ 7.0- 7.2 and salinity ≥ 26-28 %o, ambient seawater conditions remained the most suitable in rearing H. scabra juveniles for optimum growth and survival.