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UPV Theses and Dissertations

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Keyword: search.filters.keyword.Medicinal plantsSDG: search.filters.sdg.SDG 15 - Life on land

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    A preliminary investigation on the antimicrobial activity of Luffa cylindrica (Patola) leaf extract
    Benjamin, Paulette E. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2002-04)
    Four concentrations were prepared (100%, 75%, 50%, and 25%) from the Patola (Luffa cylindrical) leaf extract. Positive and negative controls were made in order to have a basis for comparison with the other four Patola leaf extract concentrations. Negative controls were purely sterilized distilled water, while the positive controls used were Amoxicillin for bacteria and Mycostatin for Candida albicans. The effectiviness of these concentrations was tested on the growth of the five selected microorganisms, namely: Staphylococcus aureus, S. epidermidis, S. saprophyticus, C. albicans and Microsporum canis. M. canis did not exhibit any growth and had no significant difference. Patola was found to inhibit the growth of S. aureus, S. epidermidis and S. saprophyticus at 100% concentration. Patola leaf concentration of 75% weakly inhibited the growth of S. epidermidis and S. aureus. Concentrations of 50%, and 25% showed negative inhibitory activity. Candida albicans in all four concentrations showed negative inhibitory activities. The minimum inhibitory activity that was able to inhibit bacterial growth was at 75% leaf extract concentration.
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    Development of a high-performance thin layer chromatography (HPTLC)-densitometric method for the estimation of quercetin and kaempferol in Euphorbia hirta (Tawa-tawa) extract
    Baladjay, Diane Monique S. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2017-06)
    A sensitive, fast, efficient, and repeatable high-performance thin-layer chromatographic (HPTLC) method has been developed for simultaneous analysis of quercetin and kaempferol from the extracts of the aerial parts of Euphorbia hirta. In the development of the HPTLC method, different extraction parameters were evaluated and quantification of quercetin and kaempferol in the extract of E.hirta was performed using a densitometric software, Quantiscan™. Among the different combinations of mobile phases used, best separation was achieved in toluene: ethyl acetate: formic acid: methanol (5: 6: 2: 1) solvent system. E.hirta was extracted using the developed extraction method which involves the use of 80% ethanol, 8 minutes of sonication and serial extraction until 3rd extraction. This determined method provides a maximum yield of the compounds at minimum time and cost. The method was also validated for linearity and precision specifically repeatability. The results show that the produced calibration is linear, as supported by the obtained correlation coefficient (r) of > 0.999 and coefficient of determination (R2) of > 0.997. Moreover, the obtained slopes of the calibration curves show that the method is most sensitive towards quercetin and least sensitive to gallic acid. The method was also found out to be repeatable and precise as the %RSD for both the inter-day and intra-day trials obtained a value less than <2%. The present method is being reported for the first time and can be used for routine quality control and quantification of quercetin and kaempferol in various plant samples, extracts, and market formulations.