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UPV Theses and Dissertations

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    Microbiological water analysis of selected point sources in Iloilo City
    Alama, Jannie Mae G.; Calitis, Ma. Socorro Fatima P.; Cervantes, Ria Angela E.; Garcia, Marian Aisa C.; Otico, Pelita A.; Pacheco, Donna Ginn G.; Taquiso, Jezreel l. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2006-04)
    The recreational use of the Iloilo River according to the Iloilo River Master Plan entails various studies to support it being safe for swimming and other activities. The Department of Environment and Natural Resources (DENR) Region VI has done very few studies to determine this particular aspect of the Iloilo River. Thus, the present study was conducted to determine the bacteriologic content of water in selected point sources in Iloilo River, Iloilo City and the possible correlations to the incidence of health risks. Specifically the study aims to: (1) determine significant differences in the physico-chemical characteristics of water samples from each point source; (2) to determine the total and fecal coliform counts number for each water sample from the selected point sources; (2) detect the presence or absence of E. coli, Salmonella spp., and culturable Vibrios in water in selected point sources; (3) describe the incidence of health risks; and (4) correlate the heterotrophic plate count for total coliforms, Most Probable Number for Fecal Coliforms and the number of symptomatic respondents. The results of the study showed that the total coliform count for Point source A (HPC=6000/l00 ml) is higher compared to the standard set by the DENR (5000/100 ml) while Point source B and C are within the range. The concentrations of E. coli, in water samples A, B, and C (2400 MPN/100 ml sample) were found to be higher than the DENR standard for fecal coliforms for Class C Waters - fresh water, (200 MPN/lOOml sample). Although results indicate that the river water has high fecal contamination, there were no significant correlations between heterotrophic plate counts, Most Probable Number for Fecal Coliforms and the number of symptomatic respondents for each point source.
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    Identification and antibiotic resistance of enterococcus (Schleifer and Kilpper-Bälz 1984) species isolated from Iloilo river
    Ansino, Jan Reygine G. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)
    The presence of Enterococci species in the water has long been regarded as an indicator of fecal contamination. With the increasing rate of antimicrobial resistance globally, infections caused by enterococcal species became difficult to treat. The study determined the species distributions and antimicrobial resistance profiles of Enterococcus species isolated from water and sediment samples in the Iloilo River. Susceptibility of the isolates was tested against ampicillin, penicillin, ciprofloxacin, chloramphenicol, doxycycline, erythromycin, tetracycline, and vancomycin. Among the 115 fecal coliform samples, a total of 61 (53%) isolates were confirmed to belong to the genus Enterococcus. Of these, 40% were identified as Enterococcus faecalis, followed by Enterococcus faecium 33% and Enterococcus avium (4%). Isolates showed resistance to tetracycline (51%), erythromycin (18%), and doxycycline (13%) and a high level susceptibility to ampicillin (100%), chloramphenicol (93%), and penicillin (90%). Multiple antibiotic resistance patterns revealed resistance to at least four antibiotics. Six (12%) vancomycin-resistant Enterococci (VRE) were detected. The results revealed low level of antibiotic resistance in the river. However, the presence of these fecal contaminants in the river can still pose a potential risk to the human health and the of spread of antibiotic resistance genes, indicating the great importance of improving risk management practices in the river.
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    Characterization and identification of previously isolated bacteria from water and sediment samples along Forbes Bridge and Sooc Bridge in Iloilo River
    Abangan, Cassey S. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2011-04)
    This study identified and characterized eighteen previously collected bacteria from water and bottom sediment samples in Forbes Bridge and Sooc Bridge along Iloilo River. Conventional method of bacterial characterization was used in this study. Morphological, physiological and biochemical tests were employed to all bacterial isolates. Identification to genus level was based on The Prokaryotes and Bergey’s Manual of Systematic Bacteriology. Four genera were identified: Staphylococcus spp. was the most abundant (50%), followed by Bacillus spp. (22.22%), Acinetobacter spp. (16.67%), and Cellulomonas spp. (11.11%). Staphylococcus spp. and Bacillus spp. were found in both sampling sites, while Acinetobacter spp. was found only in Forbes Bridge area and Cellulomonas spp. was present only in Sooc Bridge area. Three genera were identified in samples from Forbes Bridge: Staphylococcus spp. (45.5%) followed by Acinetobacter spp. and Bacillus spp.of equal distribution of 27.35%. Three genera were also identified and characterized from water and sediment samples from Sooc Bridge: genus Staphylococcus (37.14%), followed by Cellulomonas (28.67%) and lastly, Bacillus spp. (14.29%). The result of the study revealed that the two sampling sites were contaminated with bacteria. Bacterial contamination predominantly came from animal and human waste from residential units, establishments and hospitals brought to the river water directly or through runoffs.