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    Survey of pathogenic bacteria in bottom sediments of Iloilo River: An assessment of water sanitary quality
    Araneta, Karla Teresa S.; Bugayong, Adrienne Marrie S.; Cabanilla, Jennifer C.; Caro, Mary Ann Gina C.; Gayatgay, Christian Era U.; Nufuar, Ernest Jan T.; Rufino Krystal Hope S. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2009-03)
    This study assessed the water sanitary quality of the Iloilo River by determining the monthly heterotophic plate count (HPC), total coliform (TC), fecal coliform (FC), and fecal streptococcus (FS) counts of the bottom sediments in five sampling sites from October 2008 to February 2009. These parameters were then correlated with the physico-chemical parameters that were additionally obtained from the river water. The presence of Salmonella spp, E. coli. Vibrio spp, and fecal Streptococcus were also determined as indicators of the pathogenic bacteria in the bottom sediments of the Iloilo River. Using the FC:FS ratio, the study further distinguished fecal contamination sources from each site as human, animal, or mixed in origin. An assessment of sediment quality was done using DENR standards for TC and Kohl and Albinger's sediment quality criteria for HPC and FC. Sediment samples were taken from the following sampling sites along the Iloilo River: 1) Forbes. 2) Dungon, 3) IBRD, 4) Carpenter's, and 5) So-oc. No significant difference in any of the physico-chemical or microbiological parameters were found among sampling stations. However, analysis and comparison of the microbiological parameters of bottom sediments in terms of months showed significant temporal variability HPC, TC, and FC alternately decrease and increase every month while FS showed a generally increasing trend. The following correlations between the microbiological and physico-chemical parameters were found. 1) temperature with HPC and FS , 2) salinity with FS count, and 3) dissolved oxygen concentration with TC and FC Counts. The mean percentage of bottom sediment samples with Salmonella ranged from 3% to 10% while more than half of samples from all sampling sites showed the presence of Vibrio spp. and S. faecalis. Samples with E.coli from all sites ranged from 3 to 14%. Fecal contamination sources varied across sampling sites. So-oc show ed mostly animal sources as its orgin of fecal contamination, while other sites had either animal, human or mixed sources. S. faecalis and E. coli exhibited multiple antibiotic resistance, a grow ing health concent in many of today's rivers. All isolates were susceptible to Gentamicin and Ciprofloxacin but resistant to Penicillin. Based on HPC and FC values the degree of loading with easily decomposable organic substance and feces among the sampling sites ranges from very little to little. TC count of the sediment samples from all the sites does not exceed the limit of 5000 CFU/ 100ml set by the DENR. Results suggest that sediments were not a significant reservoir of bacterial pollution at the time this study was conducted.
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    Antibiotic resistance patterns of selected pathogenic bacteria isolated from water and bottom sediments in the Iloilo tidal inlet (Iloilo River)
    Agustin, Eda Mae S.; Cabrias, Ligaya April May S.; Fuentebella, Elaine Joyce V.; Gestuveo, Rommel J.; Lagon, Raymond Anthonn Kriztee J.; Saldajeno, Cypruz Anne L.; Tunguia, Saer Martin J.; Villanueva, Regene Patricia Natividad V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2010-03)
    In recent years, antibiotic resistance of pathogenic bacteria has become a public health concern. Although little is known of its environmental implications the presence of both potentially pathogenic bacteria and fecal coliform in rivers and other bodies of water raises the question of whether resistance transfer may actually occur in bodies of water. In relation, the Iloilo River for one has experienced changes in both ecological and sanitary conditions. With this threatening problem of both ecological and fecal pollution, the river could harbor microflora that could be of public health concern. Using the Kirby-Bauer Method, this study generally aims to determine the antibiotic resistance of pathogenic bacteria isolates (E. coli, S. typhi and V. cholerae) from water and bottom sediment samples from selected sampling areas in Iloilo River, Iloilo City. Stock cultures of bacterial isolates were obtained from the UPV-National Institute of Molecular Biology and Biotechnology (UPV-NIMBB) and were subjected to antibiotic resistance analysis using 12 different antibiotic classes. Results showed varying degrees of resistance in all of the sampling sites and species. Most isolates were highly resistant to penicillin and oxacillin and least resistant to ciprofloxacin. Multidrug-resistant isolates were also observed in all of the sampling sites and species. More isolates from bottom sediments were observed to be highly resistant than the isolates from water samples. Pearson correlation revealed that the antibiotic resistance patterns among the different species isolated from the different sources and sampling sites appeared to vary in a systematic manner indicative of a similar mechanism of antibiotic resistance development.