UPV Theses and Dissertations
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Item The growth and yield performance of oyster mushroom (Pleurotus ostreatus Fries) using fish wastewater as fertilizerArroyo, Karen T. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2000-04)Oyster mushroom, Pleurotus ostreatus, is an edible wood-inhabiting fungi that continues to gain popularity in the Philippines, as well as in other tropical countries. With the use of modern technology, this mushroom can be grown in the Philippines, provided sufficient moisture is present. Its present cultivation is limited, perhaps due to insufficiency of planting materials and the limited knowledge about its culture. The main objective of the study was to establish the possibility of the replacement of urea, the commonly used fertilizer for mushroom cultivation by fish wastewater. The study also aimed to compare the effects of different concentrations of fish wastewater on the growth of mushrooms and determine which treatment produced the best results. A total of five groups were used: two control groups (positive and negative - using urea and water, respectively) and three experimental groups, the latter with different concentrations of fish wastewater serving as alternative fertilizer (A: 10%, B: 20%, C: 30%). Fruiting bags were inoculated with oyster mushroom spawn, and after a month of incubation, the growing mushrooms were assessed using the parameters of number, weight, length, and diameter. Results showed that the weight of oyster mushrooms was heaviest when the substrate was sprayed with plain water. However, the number of mushrooms harvested, their length, and the width of the pilei were not affected by spraying the substrate with urea or moistening these with up to 30% fish wastewater. The conclusions reached were: 1) when using sawdust as substrate for oyster mushroom cultivation, it is best to simply use water to maintain the moisture of the material; 2) sawdust used in combination with low concentration of fish wastewater (10%) can result in good production; 3) neither nitrogen nor urea may be a critical factor for the growth of oyster mushroom on sawdust; and 4) high amount of phosphorus and low potassium content of fish wastewater are favorable for oyster mushroom growth.Item Spatial variations of Benthic meiofauna communities from Palompon Bay, Northern LeyteBalasa, Melvy June (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2013-04)Meiofauna play a significant role in maintaining ecological balance in marine ecosystems especially in the dynamics of the seagrass meadows. The meiofauna links the upper trophic level with detritus-feeding organisms. Only few studies have been conducted in the Philippines, while no studies have been. This study was conducted in Palompon, northern Leyte in November 2011, to determine the spatial distribution of meiobenthic fauna in the area and to compare community structure in terms of species composition, species density, and species diversity among five stations included in the survey. Twenty taxa were identified with nematodes being numerically dominant, followed by harpacticoid copepods. It also showed the highest mean abundance for the entire habitat type. The overall mean density was 194585.1528 ind. m2, with the highest population at station 4. And the lowest population was recorded at station 2. Soft bottom habitat showed the highest recorded mean density among the 5 stations, Harpacticoids mostly dominated sandy substrate while nematodes were widely distributed.Item Gross alpha and gross beta radioactivity and heavy metal concentration profiles of well water of the Calahunan dump site, Mandurriao, Iloilo CityAmarante, Hena Charma; Basquez, Mark Anthony; Brunio, Christian Allan; Dable, Nesel; De la Pena, Pamela-Di; Genterola, Janis; Oredina, Josette Marie; Padayhag, Dave (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2005-03)The study aimed to establish presence of radioactivity and concentration of heavy metals specifically lead and chromium in drinking water from wells within one-kilometer radius from the approximated center of the Calahunan dump site. Results showed that four out of twelve wells have positive results fro gross alpha activity and gross beta activity: wells 2A, 2C, 3A, and 3D. Well 2A, 2C and 3D registered alpha activities of 0.54, 0.033 and 0.369 Bq/L respectively while Well 3A registered beta activity of 1.0 Bq/L. Wells 2A and 3D exceeded the radiological limits set by WHO and PNSDW of 0.1 Bq/L for alpha activity and 1.0 Bq/L for beta activity. These wells that are found adjacent to the perimeter fence of the dump site registered alpha activity (wells 2A, 2C and 3D) and beta activity (well 3A). Radiological activities in all other wells sampled registered less than lower limit of detection. For gamma activity, all wells recorded 137- Cs and 134-Cs activity less than the lower limit of detection. For chromium and lead concentration using FAAS, only well 3C has a chromium concentration of 0.0952 ppm and this exceeded the PNSDW standard for chromium in drinking water (0.05 ppm) FAAS has not recorded any value for lead. For chemical constituent analysis using XRF, two wells in zone 1 (LA and IC), have recorded lead concentration of 0.9 and 0.136 ppm, respectively. In zone two, only well 2D registered a lead concentration of 0.248 ppm. In zone 3, well 3C registered a lead concentration of 0.397 ppm. For chromium concentration, only well 2A in zone 2 has a chromium concentration of 1.79 ppm. The spot interview and ocular inspection found out that the following garbage are disposed of indiscriminately tn the dump site: (1) Television picture tubes, (2) Dysfunctional Computer Monitors, (3) Used syringes and needles, (4) Hospital wastes, (5) Automobile batteries and dry cells, (6) scrap iron, tin cans, paint cans, paint cans, etc., (7) polyethylene bottle, glass bottles, and containers of similar types, and (8) other organic and inorganic wastes. Wells whose values for gross alpha, lead, and chromium concentration exceeded the standard are unsuitable for dinking and other domestic uses. The observation that wells (2A and 3D) near the Calahunan dump site registered higher-than-standard alpha activity may be a good basis to conduct further research particularly on the effect of leachate from dump site.Item Short-term impacts of oil spills on fungal population in a mangrove area in Batad, IloiloAlpas, Donna Bella C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)Oil spills cause extensive damage on the marine environment, ranging from obliteration of its flora and fauna to biomagnifications of toxic components of petroleum. The location of mangroves at the sea-land interface makes the ecosystem susceptible to disturbances such as oil spills. There is a considerable information on the impacts of oil spills on mangrove while impacts on microbial populations, especially fungi, are limited. This study therefore assessed the effect of oil on the diversity and distribution of fungi of oil-contaminated sites in Batad, Iloilo due to Power Barge oil spill last November 8, 2013. The mangrove surface sediments were collected on January 11, 2014 at oil-contaminated sites in Batad, Iloilo and uncontaminated site in Ajuy, Iloilo. Sample were processed based on Pepper and Gerba (2004). Colony forming units per gram of soil (CFU/g) was used to express yeasts and filamentous fungi. On PDA medium, more fungal isolates were isolated at oil-contaminated site compared with uncontaminated site at 14 and 12 species respectively, there was also a higher Shannon Index of Diversity at 2.4858 and 2.4582, respectively. Among the filamentous fungi, three genera were represented by Aspergillus. Penicillium, and Cladosporium. However, Aspergillus was more diverse with 10 species. Six species were found only at the oil-contaminated sites - Aspergillus sp 2. Aspergillus sp 1, Cladosporium cladosporioides, Penicillium sp 2, Penicillium sp 3 and Yeast sp 2 while four species are found in the unoiled site - Penicillium cf. lapidosum, Aspergillus sp 3, Aspergillus sp 4 Penicillium sp 1. Furthermore, there is a low index of similarity as shown by Jaccards coefficient of similarity (J=0.44) and Sorensen coefficient of similarity ( C=0.61). Fungal load was higher at oil-contaminated soils compared with uncontaminated soils at 1.3 x 10 - CFU/g and 6.0 x IO3 CFU/g, respectively. Bushnell Haas Agar yielded more fungi isolates isolated from oil-contaminated sites compared with uncontaminated site of 4 and 3 species respectively. Furthermore, a higher Shannon Index Diversity (H) was observed at oil-contaminated sites (1.3298) than those at uncontaminated site (0.6364) suggesting that there are more species of fungi capable to degrade the hydrocarbons and utilize it as a source of energy. The overall frequency of distribution of the major fungal genera in both oil-contaminated and uncontaminated sites showed that only Dueteromycota was isolated and dominated in both sites and in both media, the PDA and Bushnell Haas Agar. The major genera isolated in PDA medium included Aspergillus, Penicillium, Cladosporium, and yeast. In Bushnell Haas Agar, Aspergillus and yeast were the major genera isolated and are seen in both sites. The frequency of occurrence of various species in uncontaminated and oil-contaminated sites were not significantly different. The Deuteromycota were the dominant group that were isolated on all sites using two media. The overall result indicate the stimulating effect of oil are observed in the higher fungal load in oil- contaminated site.Item OK ka ba TIYAN? oplan kalusugan, karunungan at bayanihan tunggo sa ikauunlad ni Yong mga Ati naming kababayanAndal, Julie Jane F.; Ayongao, Jamela N.; Caton, Kate Alyssa A.; Parcon, Prem Patrick P.; Sedayon, Niña Marie Y.; Villanueva, Roena Nicole V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2013-04)World Health Organization reports that diarrhea is the fourth leading cause of death among children less than five years old and the third leading cause of illness among the children. The Philippines ranks second among thirteen countries included in the census in terms of the number of diarrhea cases causing 10,000 deaths every year. Main factors in the transmission of diarrheal diseases are: children not washing their hands due to lack of access to water and basic sanitation facilities, poor hygienic practices, and handed down practices from parents. Experts note that the incidence of diarrhea may easily be lowered by simply having kids wash their hands with water and soap. Therefore, the purpose of this project is to introduce to the community ways and means of preventing and managing diarrheal diseases by boosting the level of knowledge and introducing hygienic practices through the development of community-wide education programs that range from promoting healthy lifestyles in order to prevent disease and providing the community with safe potable water supply in Purok V- Serum Bukid, Barangay San Nicolas, Buenavista, Guimaras. Efficient uses of health services, adopt self-care practices and active participation in the design and implementation of health programs were also promoted.Item Salinity tolerance of the angelwing clam, Pholas orientalis larvaeAguaras, Lovie Grace P. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2011-03)Pholas orientalis, locally known as “diwal” is one of the commercially important bivalves in the Philippines due to its delicate and sweet taste. An experiment was conducted to determine the salinity tolerance of the 24-hour-old larvae of this clam when reared in different salinity levels of 25, 30, 35, and 40 ppt. The experiment lasted for 15 days at a rearing temperature of 25° C - 27° C. Determination of survival rates and water change were done twice every week. The data gathered were then analyzed using one-way ANOVA. Results showed that survival rates were significantly high at salinities of 30-35 ppt and were significantly low at salinity levels of 25 and 40 ppt. It was also observed that there was an increasing trend of mortality with prolonged exposure to different salinity levels. This could be attributed to the limited osmoregulatory capabilities of the larvae. When reared at low salinity levels, larvae were faced with the danger of too much water entry which eventually led to death. Meanwhile, when reared at high salinity levels, the larvae died due to outward movement of water which resulted to dehydration. Information on the salinity levels that could be tolerated by diwal larvae will be an important factor in the development of larval rearing techniques for successful hatchery operations. The juveniles or seeds produced in the hatchery could be used to restock the depleted resources in the wild.Item Antibiotic resistance patterns of selected pathogenic bacteria isolated from water and bottom sediments in the Iloilo tidal inlet (Iloilo River)Agustin, Eda Mae S.; Cabrias, Ligaya April May S.; Fuentebella, Elaine Joyce V.; Gestuveo, Rommel J.; Lagon, Raymond Anthonn Kriztee J.; Saldajeno, Cypruz Anne L.; Tunguia, Saer Martin J.; Villanueva, Regene Patricia Natividad V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2010-03)In recent years, antibiotic resistance of pathogenic bacteria has become a public health concern. Although little is known of its environmental implications the presence of both potentially pathogenic bacteria and fecal coliform in rivers and other bodies of water raises the question of whether resistance transfer may actually occur in bodies of water. In relation, the Iloilo River for one has experienced changes in both ecological and sanitary conditions. With this threatening problem of both ecological and fecal pollution, the river could harbor microflora that could be of public health concern. Using the Kirby-Bauer Method, this study generally aims to determine the antibiotic resistance of pathogenic bacteria isolates (E. coli, S. typhi and V. cholerae) from water and bottom sediment samples from selected sampling areas in Iloilo River, Iloilo City. Stock cultures of bacterial isolates were obtained from the UPV-National Institute of Molecular Biology and Biotechnology (UPV-NIMBB) and were subjected to antibiotic resistance analysis using 12 different antibiotic classes. Results showed varying degrees of resistance in all of the sampling sites and species. Most isolates were highly resistant to penicillin and oxacillin and least resistant to ciprofloxacin. Multidrug-resistant isolates were also observed in all of the sampling sites and species. More isolates from bottom sediments were observed to be highly resistant than the isolates from water samples. Pearson correlation revealed that the antibiotic resistance patterns among the different species isolated from the different sources and sampling sites appeared to vary in a systematic manner indicative of a similar mechanism of antibiotic resistance development.Item Intestinal parasitic infections in children living in selected barangays with urban slums along Iloilo RiverAguro, Eric D.; Banaag, Arlene D.; Jacobe, Venus N.; Ordaniel, Lilac Mared A.; Taleon, Kathleen Joy B.; Yandra, Marnelle Gay A. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2006-03)This cross-sectional study was performed to determine the intestinal parasitic infection in children age 0-13 years old in three selected barangays with urban slums located alongside Iloilo River. A total of 127 stool samples from 65 males and 62 females were examined by utilizing formalin-ether concentration technique (FECT) and direct wet mount thereafter using Lugol's solution. It was found out that 59.8% of the children examined were positive for one or more intestinal parasites. The most frequent soil-transmitted helminth observed was Ascaris lumbricoides (48.0%) followed by Trichuris trichiura (34.6%). Pathogenic protozoans determined were Entamoeba histolytica (9.4%) and Giardia lamblia (0.8%). Other nonpathogenic species identified were Entamoeba coli (15.7%), Blastocystis hominis (3.1%) and lodamoeba butschlii (0.8%). Parasitic infection with single species had the highest frequency of 31.5% compared to multiple infection (28.3%). The relatively high prevalence of these infections among children living three selected barangays with urban slums alongside Iloilo River was found to be independent of factors the respondents’ sex (p=0.106), age group (p=1.691), and consistency of stool sample (p=1.835). Moreover, the degree of infection in terms of sex (p=3.174), age (p=9.136), and consistency of stool samples (p=2.798) were not statistically different from each other. The high prevalence rate of pathogenic intestinal parasitic infection may have also been influenced by factors other than the ones considered, such as environmental and socio-demographic factors. The study highly suggests mass treatment and preventive measures such as health education of the communityItem Identification and antibiotic resistance of enterococcus (Schleifer and Kilpper-Bälz 1984) species isolated from Iloilo riverAnsino, Jan Reygine G. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)The presence of Enterococci species in the water has long been regarded as an indicator of fecal contamination. With the increasing rate of antimicrobial resistance globally, infections caused by enterococcal species became difficult to treat. The study determined the species distributions and antimicrobial resistance profiles of Enterococcus species isolated from water and sediment samples in the Iloilo River. Susceptibility of the isolates was tested against ampicillin, penicillin, ciprofloxacin, chloramphenicol, doxycycline, erythromycin, tetracycline, and vancomycin. Among the 115 fecal coliform samples, a total of 61 (53%) isolates were confirmed to belong to the genus Enterococcus. Of these, 40% were identified as Enterococcus faecalis, followed by Enterococcus faecium 33% and Enterococcus avium (4%). Isolates showed resistance to tetracycline (51%), erythromycin (18%), and doxycycline (13%) and a high level susceptibility to ampicillin (100%), chloramphenicol (93%), and penicillin (90%). Multiple antibiotic resistance patterns revealed resistance to at least four antibiotics. Six (12%) vancomycin-resistant Enterococci (VRE) were detected. The results revealed low level of antibiotic resistance in the river. However, the presence of these fecal contaminants in the river can still pose a potential risk to the human health and the of spread of antibiotic resistance genes, indicating the great importance of improving risk management practices in the river.Item Growth of the telescope snail, Telescopium telescopium (Linne) in brackishwater pondsApistar, Jobert H. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines, 1998-04)Samples of T. telescopium were collected every month, from April to October 1997 for 6 months, in brackishwater ponds. Allometric relationships were defined by comparing shell length with shell width, as well as various body weight proportions including total weight with shell, total volume with shell, visceral weight and visceral volume. A total of 5 cohorts with growth rates ranging from 0.45 - 0.67 mm-day- were observed using the Bhattacharya analysis. A high mean growth rate of 0.56 mm-day- (sd = 0.07, n= 5) was estimated by joining the modes of the cohorts between successive sampling months. Low inverse correlations were observed between mean shell length and various environmental parameters (i.e. salinity, air temperature, water temperature and organic matter content) possibly explained by the species' environmental adaptations. On the other hand significant correlation was observed between mean shell length and density, indicating the possible role of overcrowding in these habitats.