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UPV Theses and Dissertations

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Degree: search.filters.degree.Bachelor of Science in BiologySDG: search.filters.sdg.SDG 3 - Good health and well-being

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    A comparative study on the perceived work stress, work-related stressors, and coping strategies of fish vendors in Antique
    Cabigunda, Greshanne J.; Moquera, Mabelle M. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2024-07)
    Fish vending is a common source of income in Antique, Philippines. Fish vendors are classified into two vendor groups: formal vendors who sell inside public markets and informal vendors who sell their products on the streets. Research shows that these groups encounter varied work experiences given their differing working conditions. However, existing research is not enough to describe their stressful work experiences as well as their coping strategies. Thus, this research study aimed to describe and compare the work-related stress of fish vendor groups in Antique and how they cope with it. To address these, a convergent parallel mixed methods design was used. A total of 40 fish vendors with a mean age of 44.70 years, classified as formal (20) and informal (20), served as respondents. Data were gathered using a one-on-one interview with a question guide and a modified survey instrument for levels of perceived work stress (LPWS). Mann-Whitney U analysis revealed no significant difference between the overall LPWS of both groups (W= 4272.50, p>0.05). However, per dimension analysis of results showed a significant difference in the physical work environment dimension (W= 85.50, p<0.001). Conceptual content analysis also indicated that work-related stressors and coping strategies are relatively different between vendor groups and across dimensions. The findings of this research suggest that the physical work environment is a significant dimension that differentiates fish vendor groups’ levels and sources of work stress. Moreover, findings imply that social resources, especially among co-vendors, reduce LPWS.
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    Microbiological assessment and detection of potentially pathogenic bacteria on three areas along the Iloilo River
    Caballo, John Carlo P. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2011-04)
    The Iloilo River, which traverses the whole length of Iloilo City, serves as a water source for different human activities hence, subjecting it to a variety of pollutants. Microbial and physico-chemical parameters of the three areas along the Iloilo River (Carpenter’s Bridge, IBRD Bridge, and Dungon Creek) were assessed to in order to provide a quantifiable evaluation of the river’s water quality. The presence of Salmonella spp., Eschericia coli, Streptococcus spp. and Vibrio spp., was also determined. HPC counts indicate very-high to extremely-high levels of bacterial loading (5.31 x 105-l .60 x 106 CFU/lOOmL) among the three sampling areas. Total coliform levels indicated contamination of the river from outside sources, potentially from domestic sewage discharges and run-offs from nearby establishments. The FC: FS ratio suggests contamination mostly came from mixed origins, with the noted exception of Carpenter’s Bridge, which is dominated by human fecal contamination. Minimal variations in physico-chemical parameters were observed for all areas. Significant correlations were found among salinity (p=0.002) and temperature (p=0.042) with TC; between FS with dissolved oxygen (p=0.00), and between FC and temperature (p=0.005). Contamination of the river by organic and fecal matter from animal and human sources provided a suitable habitat for pathogenic bacteria. A considerable quantity of potentially pathogenic bacteria such as; E.coli, Salmonella spp., Streptococcus spp. and Vibrio spp., were isolated from each sampling area. Exposure to these pathogens in drinking and recreational waters poses health risks to local inhabitants and to any individual who would consume water from the river
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    The pharmacological screening of Moringa oleifera Lam.
    Burgos, Karen J. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1998-07)
    Moringa oleifera, Lam, or malunggay, belongs to Family Moringaceae. The medicinal value of this plant has long been recognized in folk medicine. Not all the claims, however, are proven scientifically. This study aimed to (1) test the crude extracts from the seeds and leaves of M. oleifera for pharmacological properties and (2) to compare its efficacy with the drugs commonly used to alleviate particular ailment. Phytochemical assay for alkaloid using Culvenor-Fitzgerald method was done to test the presence of alkaloids. The dried seeds and young leaves were extracted with methanol and concentrated under reduced pressure at 45°C. The resulting extracts were partitioned and used for the different assays: Brine shrimp assay for cytotoxicity, Acetic acid-induced writhing assay for analgesic activity, Charcoal tracing assay for antispasmodic activity and paper disc diffusion method for antimicrobial activity. Result showed that only the seeds have alkaloids. Toxicity test revealed that the seeds are practically non-toxic but the leaves were toxic with an LC50 of 550 μg/mL after 24 hours of exposure. Of the two extracts, only the leaves exhibited analgesic activity although not as effective as Mefenamic Acid in reducing pain. The leaf extract, was also found active against Bacillus subtilis, Staphylococcus aureus , Escherichia coli,and Pseudomonas aeruginosa. The seed extract was active only against B. subtilis. Both extracts, however, are not as effective as the antibiotic drug, Amoxicillin. Results also showed that both extracts do not have antispasmodic activity. The study confirmed the antimicrobial and analgesic activities for the use of the leaves of this plant in folk medicine.
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    Toxicity of Chemically Enhanced Water-Accommodated Fraction (CEWAF) of Petron bunker C oil on Asian Sea bass Lates calcarifer (Bloch) fingerlings at different salinity levels
    Bondoc, Christian Earvin D. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2011-03)
    Asian sea bass Lates calcarifer fingerlings were exposed to three CEWAF concentrations (0.3, 0.5 and 1.0%) at 28, 32 and 36%o and the mortality rate among the different treatments were compared. Dunnett t-test (a=0.05) showed that mortality in the CEWAF-exposed treatments differed significantly from that in the control, indicating that the toxicity of CEWAF caused the death of the fingerlings. The general trend of mortality was increasing with increased salinity although the results of Two-Way ANOVA (a=0.05) reveal that salinity did not influence the toxicity of CEWAF significantly. Further analysis with Fisher’s LSD test (a=0.05) showed that mortality only differed significantly between the 28%o and 36%o salinity groups. The differences between the salinity levels used in this experiment were too narrow to cause a significant difference in the interaction between CEWAF concentration and salinity. Sublethal effects of exposure to CEWAF observed in this study included aberrant swimming behavior, arching of the back, loss of buoyancy and loss of pigmentation.
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    Development of probiotics determination of LD50 of previously isolated indigenous vibriolytic bacteria, Pseudomonas aerugginosa JP4, to Penaeus monodon postlarvae
    Billena, Ma. Thesa M. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2000-06)
    Previous studies showed that Pseudomonas aeruginosa JP4 is effective in eliminating pathogenic Vibrio spp. on shrimp. However, virulence studies on this strain are lacking. Hence, this study was conducted to test the pathogenicity of P. aeruginosa JP4, using different bacterial densities of 105, 106, 107, 108, 109 and 1010 CFU/ml. These were introduced to Penaeus monodon postlarvae 13 (PL13)and early juvenile (PL25) stages. After 3 days of monitoring, mortality rates and LD50 values were determined using Reed-Muench method. Results showed that shrimps can not tolerate higher bacterial densities with LD50 of 1.15 x 107 CFU/ml and 1.12 x 108 CFU/ml for PL13 and PL25 respectively. The relatively high LD50 values on shrimp stages PL13 and PL25 indicate a low virulence of the bacterial strain. Hence, P. aeruginosa JP4 maybe used as potential vibriolytic bacteria.
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    Karyotype analysis of Kutsay Allium odoratum L. (Alliales: Alliaceae)
    Bermejo, Jo-am Asis (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1995-05)
    The C-metaphase count of Allium odoratum revealed that the chromosome number was 2n=32, consisting of the following centromeric groups based on Levan's method of chromosome classification: Group I chromosomes with subterminal centromeres (1 pair); Group II with submedian centromeres (2 pairs), and Group III with median centromeres (13 pairs). The mean total lengths of chromosome ranged from 8.534±0.504-16.624±0.600 μ. Mitotic index for the species was found to be 10.87%.
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    Wine production from different species of seaweed
    Benosa, Sophia Grace O. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2009-04)
    This study specifically aimed to test and compare the wine production of Gracilaria verrucosa, Kappaphycus alvarezii, and Kappaphycus striatum based on the physicochemical evaluation. The best seaweed wine produced was also determined through sensory evaluation. The samples underwent a 28-day fermentation period using Saccharomyces cerevisiae 2185 under room temperature at a pH of 3.0 and sugar content of 30%. Based on the physicochemical parameters, Gracilaria verrucosa was the best wine—high percentage of alcohol content, stable pH during the entire fermentation period, sweet taste of the wine owing to the high residual sugar, high amount of percent (%) TA without affecting the taste of the sample, clarity of the wine product after fermentation owing to the low total suspended solids. Gracilaria verrucosa also produced the best seaweed wine based on sensory evaluation.
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    A preliminary investigation on the antimicrobial activity of Luffa cylindrica (Patola) leaf extract
    Benjamin, Paulette E. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2002-04)
    Four concentrations were prepared (100%, 75%, 50%, and 25%) from the Patola (Luffa cylindrical) leaf extract. Positive and negative controls were made in order to have a basis for comparison with the other four Patola leaf extract concentrations. Negative controls were purely sterilized distilled water, while the positive controls used were Amoxicillin for bacteria and Mycostatin for Candida albicans. The effectiviness of these concentrations was tested on the growth of the five selected microorganisms, namely: Staphylococcus aureus, S. epidermidis, S. saprophyticus, C. albicans and Microsporum canis. M. canis did not exhibit any growth and had no significant difference. Patola was found to inhibit the growth of S. aureus, S. epidermidis and S. saprophyticus at 100% concentration. Patola leaf concentration of 75% weakly inhibited the growth of S. epidermidis and S. aureus. Concentrations of 50%, and 25% showed negative inhibitory activity. Candida albicans in all four concentrations showed negative inhibitory activities. The minimum inhibitory activity that was able to inhibit bacterial growth was at 75% leaf extract concentration.
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    A comparative study of the microorganisms found in the vaginal flora of pill and non pill user by Pap smear
    Bayate, Aimee Sarena L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-03)
    This study was conducted to identify and classify the organisms that are present in pill users and non-pill users. It will determine if taking of pills cause changes in the microflora of pill (combined) users and will describe the profile of both pill and non-pill users. The study use 200 prepared smears of the vagina, divided equally to both pill and non-pill users. The slides were collected at Dr. Ricardo Y. Ladrido Memorial Hospital, Lambunao, Iloilo by the family planning physician with the help of the medical technologist. The profile of the patients were also gathered to provide more information about the subjects. The organisms were identified according to their basic morphological characteristic. The results indicate that bacilli which is the normal inhabitant of the vagina is present in 200 samples. Cocci is present in both groups but occure more in women who are pill users. In the parasite group, Trichomonas vaginalis is present in women who are pill user and Candida is present in both groups but occur more in women who are pill users. The test for the estimation of the difference between two proportions reveal that the z values obtained from the experiment falls between the critical region which has a value of 2.58. Such a value indicate that the prevalence of parasites and bacteria are equal among pill and non-pill users.
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    Bacteriological survey and detection of Escherichia coli, Salmonella spp., Streptococcus spp., and Vibrio spp. in bottom sediments from two sites in Iloilo River
    Barrera, Christopher (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2010-04)
    Bacterial contamination in the Iloilo river occurs because of various human activities. The assessment of the river was done using microbiological parameters (HPC, TC, FC, and FS) for 7 months in Sooc Bridge and Carpenter’s Bridge using bottom sediments for samples. Sediment was used because bacteria accumulate over time in the sediment due to high amounts of organic and inorganic matter in the substrate and this leads to better survivability of bacteria. The values obtained from microbiological parameters investigated are generally higher in Sooc Bridge. HPC results show that the higher mean value was in Sooc Bridge(7.43 x 108 CFU/lOOg). The levels of TC on both sampling sites ranged from 9.5 x 102to 2.40 x 104 CFU/100g. For TC, Sooc has the higher mean (1.76 x 104 CFU/lOOg) for 7 months. FC values ranged from 3.0 x 101 to 2.4 x 104 CFU/lOOg. Sooc bridge has the higher mean(1.17 x 104 CFU/lOOg) between the 2 sites. Lastly, the FS value range from 2.9 x 103 to 2.4 x 104 CFU/lOOg. Also, Sooc Bridge has the higher mean value (1.58 x 104 CFU/lOOg). There are no significant differences on the microbiological parameters used in each site. The FC/FS ratio revealed that mixed fecal materials contaminate the river. Among the four pathogenic bacteria detected, Salmonella spp. was the most abundant for 7 months (63-65%) and E. coli was the least abundant(>l%). Based on the criteria on sediment quality evaluation, there is little to moderate loading of organic and inorganic matter.