UPV Theses and Dissertations
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Item Effects of Chromolaena odorata leaves as amendments to poultry manure and inorganic fertilizer to improve growth, yield, biomass accumulation and inorganic content of tomato (Lycopersicum esculentum)Balinas, Chona Mae D. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2012-04)Chromolaena odorata (a weed in the family Asteraceae) has been shown to enhance the buildup of organic matter in a fallow system in tropical soil. Therefore, the aim of this study was to to determine the effects of Chromolaena odorata leaves as an amendment to poultry manure and triple 14 inorganic fertilizer on the growth, yield, biomass accumulation and inorganic contents of Lycopersicum esculentum. The field experiment was conducted at Agsanayan Buenavista Guimaras where the weed has wide distribution. The leaves of C. odorata were air dried and composted using Berkeley method. The compost obtained was used as an amendment to either poultry manure and inorganic fertilizer and was tested on tomato for three months in RCBD. Obtained results proved that tested treatments with amendments of C. odorata were significantly higher in lengths of shoots and roots, flower and fruit yield, amount of biomass accumulated and inorganic contents of tomato than those with no amendments of C. odorata. Therefore, C. odorata is an efficient amendment to both organic and inorganic fertilizers. It was suggested that the weed should be utilized to serve as a green manure.Item Effect of soil amendments on damping-off disease of tomatoBalofiños, Junnel B. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1998-04)Different soil amendments obtained commercially were tested for their ability to suppress damping-off disease of tomato. Compost, animal manure, inorganic fertilizer, and green manure were applied to autoclaved soil prior to addition of a pathogenic inoculum. The biological control of the damping-off disease was evaluated by monitoring disease incidence in every plot of 50 seedlings for 25 days. Compost and animal manure significantly reduced disease incidence while green manure and inorganic fertilizer did not yield significant results as compared to the nonamended control soil. Percent emergence, dry matter content, and height were not at all affected by the different treatments.Item Spatial variations of Benthic meiofauna communities from Palompon Bay, Northern LeyteBalasa, Melvy June (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2013-04)Meiofauna play a significant role in maintaining ecological balance in marine ecosystems especially in the dynamics of the seagrass meadows. The meiofauna links the upper trophic level with detritus-feeding organisms. Only few studies have been conducted in the Philippines, while no studies have been. This study was conducted in Palompon, northern Leyte in November 2011, to determine the spatial distribution of meiobenthic fauna in the area and to compare community structure in terms of species composition, species density, and species diversity among five stations included in the survey. Twenty taxa were identified with nematodes being numerically dominant, followed by harpacticoid copepods. It also showed the highest mean abundance for the entire habitat type. The overall mean density was 194585.1528 ind. m2, with the highest population at station 4. And the lowest population was recorded at station 2. Soft bottom habitat showed the highest recorded mean density among the 5 stations, Harpacticoids mostly dominated sandy substrate while nematodes were widely distributed.Item Fungal pathogen associated with Rhizophora stylosa: A comparative study between oiled and unoiled sites within Taklong Island National Marine Reserve (TINMAR), Nueva Valencia, GuimarasBalanga-an, Kristoffer (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2009-03)Mangroves, refer to an assemblage of trees and shrubs that inhabit the coastal intertidal zone in tropical and subtropical regions. They help in the soil formation, protection, and stabilization of our coastal environment as well as serve as shelter and breeding grounds for some of our marine organisms. A mangrove habitat represents the borderline between land and sea and is considered as one of the principal places where spilled oil and associated impacts converge. Oil spill threaten mangroves by predisposing the plants to fungal attacks thereby reducing their resistance to stress and diseases. Studies also show that certain fungi species are able to utilize oil in contaminated mangrove species. This study generally aims to compare the fungal population found on the diseased leaves of Rhizophora stylosa between oiled and unoiled sites in the Taklong Island National Marine Reserve (TINMAR). It also aims to isolate, characterize and identify fungi present in diseased leaves of Rhizophora stylosa using potato dextrose agar (PDA) culture media and determine their frequency of occurrence. Leaf samples were collected from two sites in TINMAR, one of which was contaminated by oil and the other one was not. Three trees were selected to represent each of the two sampling sites (oiled and unoiled), and to each of the selected trees, three diseased leaves were collected. The results of this study showed the dominance of some species of fungi namely, Aspergillus (40%), Penicillium (20%), Cladosporium (13.33%), Monilia (6.67%), Mycelia (6.67%), Humicola (6.67%), and Pestalotiopsis (6.67%). Five out of these seven genera were isolated from the unoiled site while six of these were isolated from the oiled site. Generally, the fungal population was observed to be greater in the oiled site than in the unoiled. This preliminary data indicate that R. stylosa might still be undergoing stress due to the remaining components of the spilled oil in the area. However, this can only be resolved by further monitoring.Item Antimicrobial activity of macrofungal basidiomycetous mycelia against Vibrio spp.Bagacay, Jann Felnesh Exe V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2023-06)Aquaculture is vital for global food security, producing 50% of the world’s fish. However, infectious diseases caused by fish pathogens like Vibrio species can lead to significant production losses. To address this issue, antimicrobial agents are commonly used. Macrofungal fruiting bodies and mycelia produce bioactive compounds with antimicrobial properties. This study aims to: (a) assess antimicrobial activity in isolated macrofungal samples against the fish bacterial pathogens Vibrio parahaemolyticus and Vibrio cholerae, (b) compare two methods (agar plug and crude extracts) for antimicrobial activity, (c) determine the minimum inhibitory concentration (MIC) using agar well diffusion and the agar plug methods, and (d) identify the macrofungal isolate with the highest antimicrobial activity. Ten morphospecies were collected from the University of the Philippines Visayas Miagao Campus. The samples were evaluated using the agar plug and agar well diffusion methods. The results showed antimicrobial activity against Vibrio cholerae and Vibrio parahaemolyticus. The activity index ranged from 0 to 0.83 and 0.22 to 0.78 for agar wells, and 0 to 1.00 and 0.70 to 1.30 for agar plugs, against V cholerae and V. parahaemolyticus, respectively. Termitomyces globulus and Trametes sp. 1 exhibited the highest activity indices using the agar plug and agar well diffusion methods, respectively. Statistical analysis confirmed significant differences in antimicrobial activity against both test organisms. These findings demonstrate the potential of macrofungi as a valuable source of antimicrobial compounds for controlling fish pathogens in aquaculture. Further research could lead to the identification and utilization of these compounds to minimize production losses and improve disease management in aquaculture.Item Molluscicidal effect of quicklime, CaO on golden snail (Pomacea canaliculata)Baibado, Joewel Tarra (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1998-03)The golden snail (Pomacea canaliculata) is presently a major pest of rice in lowland and rainfed areas through out the Philippines . The problem of golden snail infestation in rice farming system includes damage to the rice plants, rice yield losses, additional expense, side effects of chemicals and destructive effect on native snails . The study was conducted to evaluate the molluscicidal effect of calcium oxide for the control of golden snails in farmlands and to determine the 96hour LC50 in order to know the lethal concentration of calcium oxide to golden snails. Calcium oxide was found to have a molluscicidal activity on golden snails The golden snails were exposed to different concentrations namely. 100 ppm. 200 ppm. 300 ppm .400 ppm. and 500 ppm Remarkable lethal effects of calcium oxide (p<0.05) has been found in 4(H) and 500 ppm concentrations which has reached 60-100 % after the 96 hour treatment. The 96-hour LC50 within the 95% confidence interval was 322.02 ppm as computed Calcium oxide is safe on rice when used as a molluscicide It performs dual function as fertilizer/soil stabilizer and molluscide. These dual effects are very significant because it means big savings on the part of the rice farmers. Considering the prevalence of this pest, farmers spend much on molluscicides and their application. With calcium oxide, the farmer has a molluscicide and soil stabilizer in one application.Item Dengue knowledge, policies, and Aedes Larval population in selected low and high dengue priority areas in Miagao, IloiloAmihan, April Rose D.; Basco, Arriane Mae P.; Escosia, Edmon B.; Monsale, April Joy P.; Solis, Jann Christian V.; Varon, Marianne Joy C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2009-03)This cross-sectional study aimed to compare the knowledge, practices, and Aedes larval populations between low and high dengue priority areas of Miagao, Iloilo. Questionnaire-guided interviews were conducted to assess the knowledge and practices of the study population. Larval surveys were done to determine Aedes larval populations, expressed as House Index (HI), Container Index (CI), and Breteau Index (BI). Results showed that respondents from the low priority area were more knowledgeable on dengue than the respondents from the high priority area (p<0.05). Dengue preventive practices were found to be the same for both low and high priority areas. There was, however, no significant relationship between dengue knowledge and dengue preventive measures (p<0.05). Results from (he larval survey revealed that both low and high priority areas have HI and BI above the WHO standards (HI<5%; BI<20) and are considered “high risk” areas. Lastly, age (p<0.01) and educational attainment (p<0.05) were found to affect an individual’s dengue knowledge.Item Community structure of mangroves in selected barangays in Sebaste, AntiqueAlorro, Mary Juandin Grace F. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2007-03)Mangroves are higher plants that are primarily found predominantly in the intertidal areas such as estuaries and lagoons of the tropical and subtropical shorelines. This study was conducted to assess the community structure of mangroves in Barangay Callan, Barangay Idio, Barangay Poblacion, Barangay Abiera, and Barangay Bacalan, Sebaste, Antique, using the transect line method. Specifically, it aimed to determine selected physical and chemical factors such as salinity, pH, temperature for water and soil as well as characterization of the substrate; identify and classify true mangrove species present in the area; and describe the community structure in terms of stand basal area, stems per hectare, relative density, relative frequency, relative dominance, importance value, Shannon index of diversity, zonation patterns, seedling and sapling density. No study has been done to document the community structure of mangroves in Sebaste, Antique thus, this study was done to provide vital information needed for its proper management. Three stations were selected in Sebaste, Antique. Four (4) species of true mangroves were identified, belonging to three (3) families: Palmae - Nypa fruticans; Rhizophoraceae - Rhizophora apiculata, Bruguiera gymnorrhiza; and Euphorbiaceae - Excoecaria agallocha. The study area had a water salinity range of 0.6 ppt to 15.3 ppt; water temperature range of 28.3°C to 35.4°C; water pH range of 7.1 to 7.7; soil temperature range of 28.3°C to 34.7°C; and soil pH range of 6.5 to 7.2. Basal area ranges from 3.16 m2/ha (Rhizophora apiculata) to 5.88 m2/ha (Excoecaria agallochd) to 8.19 m2/ha (Bruguiera gymnorrhiza) . The species with the highest average of stems per hectare were Excoecaria agallocha and Bruguiera gymnorrhiza with 300 as well as the highest average density value of 100 for Excoecaria agallocha. Nypa fruticans had the highest relative frequency and relative dominance. Among the four species, the most important species was Nypa fruticans. The Shannon Index of Dominance was 0.403 which was quite low in terms of diversity as influenced by the dominance of Nypa fruticans.Item Antibacterial activity of aqueous and methanol extracts of selected basidiomycetes from UPV, Miagao, Iloilo PhilippinesAmbubuyog, Marja Victoria U. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2013-03)The Basidiomycetes represent an enormous source for natural products with diverse chemical structures and wideranging antimicrobial activity. This study was done to investigate the antibacterial property of selected species of basidiomycetes aqueous and methanolic extracts against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Specifically, it aimed to (1) determine the antibacterial activity of the aqueous extract of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa based on the zone of inhibition; (2) determine the antibacterial activity of the methanolic extract of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa based on the zone of inhibition; and (3) compare the aqueous and methanolic extracts of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa based on the zone of inhibition. A total number of ten species were collected namely Auricularia sp., Schizophyllum sp., Trametes pubescens, Trametes versicolor, Trametes elegans, Ganoderma sp.(l), Ganoderma sp.(2), Stereum sp. and two unknowns. Dried samples were crushed and extracted with water and methanol. Antibacterial activity was determined using the Kirby-Bauer disc diffusion method. The results showed a negative to weak inhibition against the test microorganisms which is inconsistent with the results of previous studies. It could possibly be attributed to the preparation of the samples and the fruiting body maturity that influenced the antibacterial potential of the samples. It appears that fruiting bodies used in the present study may not be reliable sources of bioactive compounds for antibacterial activity.Item Species composition and distribution of mangrove soil fungi from Iloilo River, Iloilo City, PhilippinesAlmoros, Peter Paul C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2018-06)Fungi are very diverse and ubiquitous microorganisms. Fungal diversity is an important aspect of the ecosystem that needs to be studied. There have been many studies regarding biodiversity of fungi in terrestrial, marine and riverine environments, but studies regarding fungi found in mangrove soil still needs to be done. This study focused on determining the species composition, distribution and ecology of mangrove soil fungi in the Iloilo River, Iloilo City. Soil fungi were sampled from three areas in the river representing the downstream, midstream and upstream areas. Fungal isolates were identified based on morphological characteristics- cultural and microscopic features. There were 10 identified fungal species -Aspergillus cf. flavus, Aspergillus cf. niger, Aspergillus cf. ustus, Aspergillus cf. versicolor, Aspergillus sp. (1), Aspergillus sp. (2) and Aspergillus sp. (3), Geotrichum cf. candidum, Penicillium cf. citrinum and Penicillium sp. (1), while there were 2 unidentified species. Three of these species were very frequent across the sites -Aspergillus cf. flavus, Geotrichum cf. candidum and Penicillium cf. citrinum.