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UPV Theses and Dissertations

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1998 - 199912000 - 20021

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Degree: search.filters.degree.Bachelor of Science in BiologyKeyword: search.filters.keyword.Medicinal plants

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    The pharmacological screening of Moringa oleifera Lam.
    Burgos, Karen J. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1998-07)
    Moringa oleifera, Lam, or malunggay, belongs to Family Moringaceae. The medicinal value of this plant has long been recognized in folk medicine. Not all the claims, however, are proven scientifically. This study aimed to (1) test the crude extracts from the seeds and leaves of M. oleifera for pharmacological properties and (2) to compare its efficacy with the drugs commonly used to alleviate particular ailment. Phytochemical assay for alkaloid using Culvenor-Fitzgerald method was done to test the presence of alkaloids. The dried seeds and young leaves were extracted with methanol and concentrated under reduced pressure at 45°C. The resulting extracts were partitioned and used for the different assays: Brine shrimp assay for cytotoxicity, Acetic acid-induced writhing assay for analgesic activity, Charcoal tracing assay for antispasmodic activity and paper disc diffusion method for antimicrobial activity. Result showed that only the seeds have alkaloids. Toxicity test revealed that the seeds are practically non-toxic but the leaves were toxic with an LC50 of 550 μg/mL after 24 hours of exposure. Of the two extracts, only the leaves exhibited analgesic activity although not as effective as Mefenamic Acid in reducing pain. The leaf extract, was also found active against Bacillus subtilis, Staphylococcus aureus , Escherichia coli,and Pseudomonas aeruginosa. The seed extract was active only against B. subtilis. Both extracts, however, are not as effective as the antibiotic drug, Amoxicillin. Results also showed that both extracts do not have antispasmodic activity. The study confirmed the antimicrobial and analgesic activities for the use of the leaves of this plant in folk medicine.
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    A preliminary investigation on the antimicrobial activity of Luffa cylindrica (Patola) leaf extract
    Benjamin, Paulette E. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2002-04)
    Four concentrations were prepared (100%, 75%, 50%, and 25%) from the Patola (Luffa cylindrical) leaf extract. Positive and negative controls were made in order to have a basis for comparison with the other four Patola leaf extract concentrations. Negative controls were purely sterilized distilled water, while the positive controls used were Amoxicillin for bacteria and Mycostatin for Candida albicans. The effectiviness of these concentrations was tested on the growth of the five selected microorganisms, namely: Staphylococcus aureus, S. epidermidis, S. saprophyticus, C. albicans and Microsporum canis. M. canis did not exhibit any growth and had no significant difference. Patola was found to inhibit the growth of S. aureus, S. epidermidis and S. saprophyticus at 100% concentration. Patola leaf concentration of 75% weakly inhibited the growth of S. epidermidis and S. aureus. Concentrations of 50%, and 25% showed negative inhibitory activity. Candida albicans in all four concentrations showed negative inhibitory activities. The minimum inhibitory activity that was able to inhibit bacterial growth was at 75% leaf extract concentration.