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UPV Theses and Dissertations

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    Characterization and identification of previously isolated bacteria from water and sediment samples along Forbes Bridge and Sooc Bridge in Iloilo River
    Abangan, Cassey S. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2011-04)
    This study identified and characterized eighteen previously collected bacteria from water and bottom sediment samples in Forbes Bridge and Sooc Bridge along Iloilo River. Conventional method of bacterial characterization was used in this study. Morphological, physiological and biochemical tests were employed to all bacterial isolates. Identification to genus level was based on The Prokaryotes and Bergey’s Manual of Systematic Bacteriology. Four genera were identified: Staphylococcus spp. was the most abundant (50%), followed by Bacillus spp. (22.22%), Acinetobacter spp. (16.67%), and Cellulomonas spp. (11.11%). Staphylococcus spp. and Bacillus spp. were found in both sampling sites, while Acinetobacter spp. was found only in Forbes Bridge area and Cellulomonas spp. was present only in Sooc Bridge area. Three genera were identified in samples from Forbes Bridge: Staphylococcus spp. (45.5%) followed by Acinetobacter spp. and Bacillus spp.of equal distribution of 27.35%. Three genera were also identified and characterized from water and sediment samples from Sooc Bridge: genus Staphylococcus (37.14%), followed by Cellulomonas (28.67%) and lastly, Bacillus spp. (14.29%). The result of the study revealed that the two sampling sites were contaminated with bacteria. Bacterial contamination predominantly came from animal and human waste from residential units, establishments and hospitals brought to the river water directly or through runoffs.
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    Bacteriological analysis of seawater from San Salvador to Belen bay areas in Banate Bay, Banate, Iloilo
    Abancio, Celyn Ann L. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines in Visayas, 2000-04)
    This study was conducted at Banate bay, Banate, Iloilo from a point from Belen to San Salvador. Two samplings were taken one week apart and was analyzed for the Most Probable Number (MPN) per ml coliform count The Multiple fermentation tube technique showed that in Poblacion approximately 100 meters from the shore had the lowest MPN values for both samplings which was >3 MPN per ml. The highest count of greater than or equal to 2400 MPN per ml was obtained in sites Belen-10, Belen-50, and Bularan-10 meters away from the shore. As the distance from the shore increases, the MPN values usually decreases, showing that the area near the shore would exhibit high coliform counts than the are further away from the shore. The tide and the weather may have affected the coliform counts because of the differences of MPN values.