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UPV Theses and Dissertations

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    Antidiarrheal activity of Pterocarpus indicus bark extracts using castor oil-induced diarrhea model on mice
    Cabatea, Norberto C. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2010-04)
    This study evaluated the antidiarrheal activity of the bark of Pterocarpus indicus (Narra). The crude methanolic extract of the bark was subjected to sequential extraction using hexane, DCM, and ethyl acetate. These extracts were tested for antidiarrheal activity in vitro using the castor oil induced diarrhea model in mice employing charcoal tracing method. Thin Layer Chromatography (TLC) was used in determining the chemical constituents present in every extracts using spraying reagents to aid in the visualization of spots. Crude methanolic extract dosage 200 mg/kg body weight is the comparable dosage to the reference drug loperamide (2 mg/kg body weight). The hexane extract was the most active fraction and 200 mg/kg dosage was more effective than with the positive control loperamide (2 mg/kg). No antidiarrheal activity found in the fraction of DCM and ethyl acetate extracts. Phytochemical screening revealed positive tests for tannins, phenolics, terpenes, cardiac glycosides, saponins and reducing sugars in all fractions. Tests for flavonoids and alkaloids, however, were negative.
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    Comparative analysis of the best practices in ecosystem-based watershed planning and management in the Philippines and Canada
    Cabios, John Paul G.; Untaran, Paul Joseph N. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2015-06)
    The Philippines is one of the many examples of a developing state vulnerable to natural disaster that erodes soil and water quality. With the continuous evolution of watershed management practices and the growing importance of climate change mitigation, there is a need to reassess the structure, function, and policy approach of the different stakeholders. And due to climate change and anthropogenic impacts, the environment especially watershed and agricultural farms in Canada are greatly affected. Because of this, there is a need to create environmental management practices to mitigate and minimize the effects of climate change and human interventions. The objectives of this paper is (1) to provide an analysis of the best watershed management practices in Canada and Philippines, (2) to identify governance structures and inter-governmental coordination, and (3) to determine planning approaches and community and stakeholder engagement. This paper will discuss generic models of governance in environmental policy and the different approaches to water management in both countries. This paper will also compare the differences between the models and approaches used in the two countries. And lastly, this paper will evaluate the use of watershed management practices in Canada and Philippines in order to find out the most compatible and reliable method or approach of watershed management.
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    In vitro antibacterial activity of Hibiscus rosa sinensis leaf extracts against some pathogens associated with urinary tract infections
    Cabayao, Coney B. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2011-04)
    The in vitro antibacterial activity of Hibiscus rosa sinensis leaf ( methanolic, dichloromethane, ethyl acetate, n-hexane, and aqueous ) extracts were studied against Gram-negative (Escherichia coli and Pseudomonas aeruginosa ) and Gram-positive ( Streptococcus faecalis) bacteria. The methanolic extract was subjected to sequential liquid-liquid extraction using solvents of varying polarity and concentrations. Out of the 5 extracts, methanolic and dichloromethane extracts exhibited the highest antibacterial activity as measured using the zone of inhibition. This was followed by ethyl acetate and n-hexane extracts. At 200 mg/ml concentration, all extracts were considered active when tested with E. coli and S. faecalis, and were partially active against P. aeruginosa. The aqueous extract was inactive towards the three bacteria. The 200 mg/ml concentration showed no significant difference when compared with the positive control, amoxicillin (12.51μg/ml). This shows that Hibiscus rosa sinensis exhibits a promising antibacterial activity. Phytochemical analysis of the active fraction- the methanolic extract- showed the presence of alkaloids, tannins, glycosides, saponins, steroids, flavonoids, terpenoids, and anthraquinones. These bioactive components could be responsible for the antibacterial activity of Hibiscus rosa sinensis leaf extracts.
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    Comparative development of larval performance of catfish hybrids (Clarias macrocephalus Gunther x Clarias gariepinus Burchell)
    Cabanos, Cerrone Salamat (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2003-03)
    Our native catfish, Clarias macrocephalus, is one of the most well-accepted fish in the country. With its soft and savory flesh, this species has high demand in local markets. However, due to its relatively slow growth and poor breeding activity, our native catfish has become rarely used nowadays in aquaculture. This led to the introduction of exotic and faster growing species like the African catfish, Clarias gariepinus, and Thai catfish, Clarias batrachus, which in turn, resulted to the near extinction of the native species. There is therefore a perceived need to undertake a breeding program involving this native catfish and other exotic species. One potential solution to increase our production rate of the native catfish is to hybridize it with the African catfish. Interspecific hybridization has been a popular breeding program utilized by researchers attempting to produce fish expressing hybrid vigor (positive heterosis) or for combining valuable traits from more than one species. This study is on the characterization of the early developmental stages of fertilized eggs and the comparison of larval performance (in terms of percent fertilization, hatching and larval survival) resulting from the artificial cross of Clarias macrocephalus (native catfish) and Clarias gariepinus (African catfish) and its parental species. In comparing the rates of egg development among crosses (all macrolecithal and discoidal cleavage), it was determined that fertilized eggs of the standard hybrid cross (C. gariepinus male x C. macrocephalus female) have a relatively faster rate of development than those from the reciprocal cross (C. macrocephalus male x C. gariepinus female). Results on determination of larval performance showed that there is no significant difference in percent fertilization and percent survival after 7 days between hybrid and parental crosses. However, in terms of percent hatching, the reciprocal hybrid cross (C macrocephalus male x C. gariepinus female), together with the C. gariepinus x C. gariepinus parental cross had a significantly higher percent hatching (P < 0.01) compared to the standard cross (C. gariepinus male x C. macrocephalus female).
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    Habitat-type and temporal comparison of the meiobenthic composition of Taklong Island National Marine Reserve
    Burgos, Lorelie A. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2009-03)
    Meiofauna are very important indicators of human disturbances in the natural ecosystem. It plays a major role in the primary production, rapid nutrient cycling and other benthic chemical processes. Knowledge regarding marine benthic communities is scarce and is limited only to temperate regions. Moreover, only a few have been conducted in the Philippines, while no studies have been conducted in the Taklong Island National Marine Reserve, Nueva Valencia, Guimaras, so this serves as the baseline for further studies to be conducted in the place. This study provides a characterization of the spatial and temporal distribution of meiobenthic fauna in the Taklong Island National Marine Reserve. Specifically, the study aimed to compare the composition of meiobenthic infauna in different habitat types and to examine changes in their abundance and distribution from 4, 16 and 26 weeks after the oil spill. Meiobenthic samples were collected in Taklong Island National Marine Reserve during the Rapid and Monitoring Phases of the Benthos Project of the UPV Oil Spill Response Program. Surveys were conducted in September 2 and November 23, 2006 and February 9 2007, respectively 4, 16 and 26 weeks after the spill. Nine stations within the area were chosen based on the station grid for the biological surveys. A total of 20 taxa were identified, with nematodes being numerically dominant. It also showed the highest mean abundance in all the sampling period and for the entire habitat type. The over all mean density was (80276.71494 ind. m2, SD =19988.37), with the highest peak in February 20072007 which is 26 weeks after the oil spill (143,508.4 ind/m2). And the lowest density was recorded in the month of September 2006, 4 weeks after the oil spill (47,205.43 ind. m2). For the habitat type soft bottom has the highest recorded mean density in the entire sampling period. For the temporal variation food source, organic matter and temperature dictates the changes in distribution of meiofauna but in the study it mostly because of the oil spill happened before the sampling period.
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    Abundance, composition and vertical distribution of calanoid copepods in Central Sulu sea
    Burgos, Lora A. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2018-06)
    Calanoid copepods are dominant component of the copepod community, which in turn highlights their influence on the overall zooplankton assemblage of the water column. Studying their abundance and distribution, is important on understanding their role in the dynamics of commercially important organism like the fish. Calanoid samples in study, were part of the zooplankton samples taken during the Joint PhilEx oceanographic cruise on board RAZ Melville in December 2007 in the Central Sulu Sea from MOCNESS station 29. Calanoid abundance, composition and distribution were investigated. Fifteen (15) calanoid families were identified all throughout the water column (0 - 400 m). The calanoid assemblage was dominated by 27 % Eucalanidae, 12 % Rhincalanidae, Scolecitrichidae with 7 % and other families contributing to roughly 19 % of the total. The water column was divided into 2 distinct depth cluster based on species distributions; (1) layers above thermocline (0 - 150m) and (2) deeper layers (200 - 400m). Furthermore, upper layers were characterized by distinctive calanoid family assemblage which includes; Calanidae, Euchaetidae, Eucalanidae, Candaciidae, Rhincalanidae, and Temoridae. While deeper layers, calanoid families includes; Metridinidae, Scolecitrichidae, Rhincalanidae, and Euchaetidae. ANOSIM results showed significant differences between these depth clusters, with global R = 0.81 and p = 0.01. These appear to be highly related to the thermocline in both stations, where typically higher upper-layer primary productivity can support dense communities of zooplankton.
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    The pharmacological screening of Moringa oleifera Lam.
    Burgos, Karen J. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1998-07)
    Moringa oleifera, Lam, or malunggay, belongs to Family Moringaceae. The medicinal value of this plant has long been recognized in folk medicine. Not all the claims, however, are proven scientifically. This study aimed to (1) test the crude extracts from the seeds and leaves of M. oleifera for pharmacological properties and (2) to compare its efficacy with the drugs commonly used to alleviate particular ailment. Phytochemical assay for alkaloid using Culvenor-Fitzgerald method was done to test the presence of alkaloids. The dried seeds and young leaves were extracted with methanol and concentrated under reduced pressure at 45°C. The resulting extracts were partitioned and used for the different assays: Brine shrimp assay for cytotoxicity, Acetic acid-induced writhing assay for analgesic activity, Charcoal tracing assay for antispasmodic activity and paper disc diffusion method for antimicrobial activity. Result showed that only the seeds have alkaloids. Toxicity test revealed that the seeds are practically non-toxic but the leaves were toxic with an LC50 of 550 μg/mL after 24 hours of exposure. Of the two extracts, only the leaves exhibited analgesic activity although not as effective as Mefenamic Acid in reducing pain. The leaf extract, was also found active against Bacillus subtilis, Staphylococcus aureus , Escherichia coli,and Pseudomonas aeruginosa. The seed extract was active only against B. subtilis. Both extracts, however, are not as effective as the antibiotic drug, Amoxicillin. Results also showed that both extracts do not have antispasmodic activity. The study confirmed the antimicrobial and analgesic activities for the use of the leaves of this plant in folk medicine.
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    Economic valuation of seagrasses in Nueva Valencia, Guimaras: A contingent valuation study
    Bundal, Kristine Shiela Mae B.; Taperla, Roald Ray B. (Division of Social Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2015-06)
    This study assessed the willingness of the residents of Guimbal, Iloilo, to pay for the conservation of seagrasses in Nueva Valencia, Guimaras. The study was conducted to find solutions to the problem of growing seagrass decline in the area. The lack of necessary policies or programs and conservation financing, that would protect and improve the integrity of the resource are putting a lot of pressure not only on the associate resources, but also on the livelihood of the people who are dependent on the resource. The contingent valuation method (CVM) was used to elicit people’s willingness-to-pay to contribute to a conservation trust fund for the protection and improvement of the state of the estimated 20- hectare area of seagrasses in Barangay Tando. It has been under serious threats of rapid decline ever since the occurrence of the 2006 M/T Solar-1 oil spill, which also affected the area. The study found out that 89.32% of the respondents would be willing to pay to support the conservation of the seagrass ecosystem. The study also found out that the primary motivation behind the willingness-to-pay of the respondents was their concern for the benefit of future generations. In addition, based on the people’s WTP, an estimated amount of PHP 900,917.64 could be raised annually to fund or support the conservation of the seagrasses and its associate resources in Barangay Tando. Given this potential source of revenue, the residents preferred a payment collection scheme through additional taxes on property or utility bills like electricity bill. Furthermore, this study reveals pertinent socioeconomic and conservation financing implications that need to be considered in managing seagrass resources.
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    Mangrove community structure survey in Barangay Guisi and Linayasan, Altavas, Aklan
    Bullo, Gliford G. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2004-04)
    The mangrove forest is a complex and unique community of medium to large tress, shrubs, vines and palms, oddly thriving with luxuriant growth in the least inviting habitats which have the barest and limited conditions for the growth and survival of higher plant life. This study aims to assess the community structure of mangroves in Barangay Guisi and Linayasan, Altavas, Aklan using accepted ecological indicators. The sampling site, Altavas, Aklan is located at the upper median of Panay Island. There was a total of 15 true mangrove species belonging to the 9 families in the area. The nine families are Family Myrsinaceae represented by Aegiceras corniculatum, Family Avicenniaceae represented by Avicennia alba, Avicennia marina, Avicennia officinalis and Avicennia rumphiana; Family Rhizophorceae represented by Rhizophora apiculata, Rhizophora mucronata, Bruguiera gymnorrhiza and Bruguiera parviflora; Family Bombacaceae represented by Camptostemon phihppensis; Family Rubiaceae represented by Scyphiphora hydrophylaceae; Family Meliaceae represented by Xylocarpus granatum and Family Palmae represented by Nypa fruticans. Avicennia rumphiana has the highest mean basal area of 23.21 (m2/ha), while Xylocarpus granatum had the lowest mean basal area of 0.01 (m2/ha). Avicennia rumphiana has the highest relative density of 57, while Xylocarpus granatum has the lowest relative density with a value of 1. Avicennia rumphiana and Avicennia officinalis had the highest relative frequency of 19.8667 Aegiceras corniculatum, Excoecaria agallocha, Scyphtphora hydrophylaceae and Xylocarpus grunatum on the other hand had the lowest relative frequency of 0.4. Avicennia rumphiana is the dominant species with a relative dominance value of 23.66, and Xylocarpus granatum is the least dominant species with relative dominance value of 0.01. Avicennia rumphiana is the most important species in the area with an importance value of 127.1816, and the least important species, as observed, is Xylocarpus granatum with an importance value of 1.685863. The index of diversity is 0.918655 for the entire area.
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    The adsorption capacity of silica gel and rice hull ash on phenol and aniline
    Bulatao, Ben Fermin P. (Department of Chemistry, College of Arts and Sciences, University of the Philippines Visayas, 2011-04)
    Chemical waste has been a problem here in the University of the Philippines Visayas, Miagao, Iloilo. Various methods of removal have been utilized. Extraction of silica gel from rice hull and rice hull ash was used to remove organic compounds (phenol and aniline). In this study, silica gel and rice hull ash adsorption on phenol and aniline were investigated with varying contact time and initial concentration. Silica gel reaches its optimum adsorption of both phenol and aniline at 60 minutes contact time. Silica gel was the basis for rice hull ash’s contact time because rice hull ash is 90% by weight silica gel.. Silica gel-phenol system decreases it adsorption capacity as the initial concentration is increased from 25 ppm to 100 ppm. Rice hull ash- phenol system, on the other hand, shows that the adsorption capacity increases as the initial concentration is increased. Both on silica gel-aniline system and rice hull ash- aniline system have increasing adsorption as initial concentration is increased. As the contact time is increased, adsorption increases only until it reaches its optimum adsorption which is 60 minutes contact time where saturation is reached. Increasing initial concentration however has different effect on adsorption on all system explained by the adsorption isotherms. The isotherms show that phenol has the fastest rate to form a monolayer (K and Q°) with the greatest binding strength (b) on silica gel. It is also observed that phenol and aniline formed a monolayer (1/n) on the surface of the silica gel and rice hull ash.