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UPV Theses and Dissertations

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    Quality changes in Tilapia (Oreochromis niloticus) after delayed icing and subsequent storage in ice
    Saluan-Abduhasan, Fathma (College of Fisheries, University of the Philippines in the Visayas, 1985-04)
    Quality changes in tilapia (Oreochromis niloticus) iced directly on harvesting were compared to those occurring when icing was delayed. Chemical and sensory examinations showed that the shelf life of tilapia was 26 days when iced immediately. 20 days and 16 days with 4 and 8 hours delay, respectively. Fish kept at ambient temperature for 12 hours were rejected after one day in ice. These results suggest that for every hour delay in icing the effective shelf life is reduced by approximately one day up to the 8th hour delay. Total volatile nitrogen values increased with storage time. Likewise, K-value and hypoxanthine values increased as storage time increased. The initial bacterial load ranged from 103- 104 cfu/g of fish with H2S-producers count of less than 100 cfu/g of fish. At rejection, the microbial load was 108~109 cfu/g of fish with HgS-producers count of 106-107 cfu/g. The typical off odours of naturally spoiling fish have been reproduced in blocks of sterile muscle by organisms such as Alteromonas putrefaciens, Pseudomonas sp, pseudomonas maltophilia, Pseudomonas flourescens and pseudomonas stutzeri.
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    The effect of common table salt added in different levels of water salinity as culture medium on growth and survival of prawn larvae Peneus monodon Fabricius
    Abeto, Mario N. (College of Fisheries, University of the Philippines in the Visayas, 1989-09)
    A study was conducted on the effect of common table salt added in waters of different salinity levels used to determine the survival rate, developmental stage, and carapace length of Penaeus monodon larvae from zoea one to post larva one. The following treatments which were adjusted to 33 ppt by adding varying amount of common table salt were: treatment I (8 ppt + 29 g/l) , treatment II (13 ppt + 23 g/l) , treatment III (18 ppt + 17 g/1), treatment IV (23 ppt + 11 g/1), and treatment V (28 ppt + 5/g) . Natural seawater with salinity of 33 ppt was used as control. Certain key ions like Na+, CL-, Ca2+, and Mg2+ were analyzed in each treatment. A sharp drop of. population in all treatments was recorded in the first 5 days of the zoeal stage. Treatment V gave the highest survival rate (25%) , but not significantly higher than treatment IV (20.5%) and control (18%); however, significantly higher than treatment III (10.5%) and treatment II (6.5%) at P<0.05. None of the larvae survived in treatment I after 5 days. Larvae exposed to treatment V had completed post larva one in 11 days (growth index 7). This was not significantly different, however, from those obtained from the control (growth index 6.6) and treatment IV (growth index 6.3). The lowest growth index value which was significantly lower than treatment V was obtained from treatment II and treatment III (growth index 6.0). During the first sampling, carapace length in treatment I reached 0.47 mm, succeeded by the following treatments: III (0.43 mm) , II (0.42 mm) , and V (0.40 mm). Treatment IV and control gave similar results (0.30 mm). After 2 days, rate of increase of CL had shifted from lower to high ionic concentrations culture media. At the end of the study the following carapace lengths were obtained: 1.2 mm for treatment V, 1.15 mm for control, and 1.08 mm for treatment IV. Values obtained from treatment II (0.94 mm) and treatment III (0.98 mm) were significantly lower than treatment V (1.15 mm). Rearing waters with higher concentrations of K+ and Mg2+ (control, treatments I, and II)showed better growth rate and percent survival than those media with relatively lower concentrations (treatments I, II, and III) . The addition of common table salt to low saline waters, however, did not compensate for the loss of physiologically important ions needed for growth and survival of prawn larvae.
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    The effects of different salinity and organic matter levels on the growth of blue-green algae
    Bantillo, Rosario R. (University of the Philippines Visayas, 1983-03)
    A one-month culture of lab-lab to determine the growth of blue-green algae in three levels of salinity ( 15 ppt = S1, 30 ppt = S2 and 45 ppt S3) and two levels of organic matter (3.4 to 4.4% = OM1 and 6.5 to 7.8% = OM2) was conducted using a twenty-two plastic containers (diameter = 43 cm; height = 49 cm) at the University of the Philippines in the Visayas Brackishwater Aquaculture Center, Leganes, Iloilo. The study utilized a 3 x 2 factorial experiment in completely randomized design with three replicates. Organic matter levels of the soil affected the chemical properties of soil and water (available phosphorus, reactive phosphorus, ammonia-nitrogen and pH). The higher the organic matter level, the higher the pH and the concentrations of ammonia and phosphorus. On the other hand, salinity did not apparently affect the fluctuations in ammonia, phosphorus and pH due to water replenishment to maintain treatment levels of salinity. Earlier rapid growth of blue-green algae commencing on the 3rd to the 15th day was observed at higher organic matter level. While at lower organic matter level, this occurred only on the 18th to the 28th day. Blue-green algae population (units/ml) was observed highest in treatment IV (S1 OM2; 1,262,113) followed by treatments III (S3OM1 761,338); II (S2OM1; 514,788); I (S1 OM1 494,375); V (S2OM2; 413,750); and, the lowest was obtained from treatment VI (S3OM2; 394,275). The relative percentage proportion of blue-green algae in the lab-lab complex showed that it composed 17.7% in treatment IV, 16.86 % in treatment III, 16.64% in treatment II, 15.82% in treatment I, 15.2% in treatment V and 14.9% in treatment VI. These, however, did not significantlyvary among treatments. A low correlation coefficient (r) between the population count and the selected parameters: reactive phosphorus, water pH, soil pH and available phosphorus was obtained while a negative correlation was obtained between the population and ammonia-nitrogen. Significantly higher biomass (ash-free dry weight) of lab-lab was obtained in higher organic matter (OM2) and at 45 ppt salinity (S3). The highest biomass (ash-free dry weight) was obtained in treatment VI (S3OM2; 0.160 g/cm2) while the lowest was obtained in treatment I (S1OM1 0.012 g/cm2). The algal count of lab-lab also showed a significant difference in the organic matter levels but no significant difference on the salinity levels. The highest algal count was obtained in treatment V (S2OM2; 6,773,542 units/ml) and the lowest count was obtained in treatment II (S2 OM1; 2,328,792 units/ml). However, the primary productivity based on O2 production showed a significantly higher production in the lower organic matter and at higher salinity level (30 ppt.). The highest O2 concentration was obtained in treatment III (S3OM1 5.759 ppm) and the lowest was obtained in treatment V (S2 OM2; 4.19 ppm).
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    Effect of cow and chicken manures on milkfish Chanos chanos (Forsskal) production in brackishwater ponds
    Aduma, Ignatius A. (College of Fisheries, University of the Philippines in the Visayas, 1984-05)
    A study using chicken and cow manures with basal application rates of 0.5 , 1,2 and 4 tons/ha was conducted. The effect of both manures on some selected physico­-chemical properties of the pond soil and water, primary productivity, and milkfish Chanos chanos (Forsskal) yield were compared. Using 8 treatments with 3 replicates each in a completely randomized design, 24 units of 40 m2 ponds were stocked with 20 fingerlings/pond. The fish were cultured from September 22 to December 20, 1983 at the Brackishwater Aquaculture Center of the University of the Philippines in the Visayas College of Fisheries, Leganes, Iloilo, Philippines. The differences among treatments in terms of all physico-chemical parameters monitored were not significant except for the reactive phosphorus. Primary productivity in all treatments was at its peak during the 5th week, after which, it declined gradually apparently due to the combined effects of cloudy weather and low concentration of reactive phosphorus in the pond water. The highest mean net fish yield (680.9 kg/ha) and mean growth rate (1,54 g/day) were obtained in treatment 3 with 1 ton/ha of chicken manure. Treatment 2 (0*5 ton/ha of cow manure) had the lowest mean net fish yield (343.6 kg/ha) and likewise the lowest growth rate (0.80 g/day). The survival in all treatments was high, ranging from 95 to 100%. In general, chicken manure was superior to cow manure, however, fish production from the treatment with 2 tons/ha of cow manure was comparable to that of 2 tons/ha of chicken manure suggesting that cow manure could be used as organic fertilizer in brackish- water ponds for milkfish production.