UPV Digital RepositoryUPV-DRUniversity of the Philippines Visayas
 

UPV Theses and Dissertations

Permanent URI for this communityhttps://hdl.handle.net/20.500.14583/10

Browse

Filters

2005 - 20062

Settings

Committee Chair: search.filters.chair.Pahila, Ida

Search Results

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Cytotoxicity and anti-vibriotic activity of Noni as an ethnobotanical
    Arrojo, Marley C. (Division of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Visayas, 2005-12)
    The crude methanolic extract of Acacia, Alibutbut, Bayabas, Biab, Botolan, Bulobitu-un, Buyo, Kamunsil, Kasla, Kayos, Langka-langka, Noni, Pasaw, Sampaguita, Tabong, Talisay, Yellow bell, were bioassayed for their cytotoxicity using brine shrimp nauplii assay. Among the plant extracts, Noni gave the highest LCso equal to 943.329 pg/mL. Noni was the least toxic among the plants tested and its crude methanolic extract was found to have activity against V. harveyi with an anti-vibriotic index of 0.256. Sequential extraction using solvents with increasing polarity gave fractions that exhibited anti-vibriotic property but the more polar ethyl acetate fraction gave the highest anti-vibriotic index of 0.513. It appears that the anti-vibrio constituent is polar. Using VLC, the ethyl acetate fraction was further separated into eighteen fractions which were in turn pooled into five fractions, Fa, Fb, Fc, Fd, and Fe based on their TLC profile. Anti-vibriotic activity of each fraction was determined. Fraction Fa has the highest anti-vibriotic index (0.192) but this is much lower than 0.513. There must be some synergy among the polar constituents that exhibited a higher anti-vibriotic index in the ethyl acetate fraction compared to fraction Fa. Based on several color reactions, ethyl acetate fraction may contain any of the following plant metabolites such as tannins, saponins, phenolic groups, conjugated double bonds, flavonoids, sterols, terpenes, except alkaloids.
  • Thumbnail Image
    Item
    Fourier transform -infrared-attenuated total reflectance spectroscopy in conjunction with the principal component analysis and agglomerative hierarchical clustering to classify virgin coconut oil and other edible oils
    Aloba, Eugene J. (Division of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Visayas, 2006-04)
    This work investigates the potential of Fourier Transform Infrared-Attenuated Total Reflectance (FTIR-ATR) Spectroscopy in conjunction with Principal Component Analysis (PCA) and Agglomerative Hierarchical Clustering (AHC) to classify virgin coconut oil (VCO) from other different edible oils in the test set. Among the PCA types, Pearson produced the best biplot graphs that showed both the separation of VCO and coconut oil (only in one group) from other oils, and the separation of VCO and coconut oil (CCO) into two distinct groups using the fingerprint region of the spectra. Kendall dissimilarity/Ward’s method successfully clustered VCO and CCO as separate classes using the same region of the spectra. These results showed that the highest percent variability in the spectra of different oils occurs in the fingerprint region, and hence where spectroscopic classification of oils can be performed best.