UPV Theses and Dissertations
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Item Endophytic fungi from Halimeda macroloba and Sargassum sp.in Nueva Valencia Nueva Valencia, Guimaras, PhilippinesAlemania, Darlene Kris V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2019-06)Endophytic fungi of marine algae in the Philippines have not been widely explored despite the fact that marine fungi associated with algae are the second most diverse. This study aimed to conduct a preliminary investigation on the occurrence of endophytic fungi from Halimeda macroloba and Sargassum sp. In this study fungal endophytes were isolated from Halimeda macroloba and Sargassum sp. collected from Nueva Valencia, Guimaras. Broad-leaf macroalagal samples were surface sterilized and cultured on PDA seawater media at 37°C. Isolated fungal endophytes were identified through colony and microscopic characteristics. Twenty one distinct endophytic fungi were isolated, 17 of which were identified up to the genus level with the most endophytes belonging to the genus Aspergillus. Fungal endophytes obtained were mostly ascomycetes specifically Aspergillus sp., one hyphomycete and three unidentified fungi with sterile mycelia were present. The brown algae, Sargassum sp., had a higher diversity compared to the green algae Halimeda macroloba in spite of having a lower isolation frequency. In terms of similarity, the two algal hosts had a low index of similarity with J= 10%. This study contributes to the growing data regarding diversity and abundance of fungal endophyte species from marine macroalgae in the Philippines. This is the first study conducted on fungal endophyte assemblages of algae species in Western Visayas.Item Pesticide exposure, Knowledge, Attitude and Practices (KAP), and laboratory findings of farmers of Barangay San Ambrosio, Igbaras, IloiloAcuesta, Doneza Dee L.; Bernaldo, Alyka Joy D.; De la Pena, Leobert Julienne A.; Diestro, Joan Marie T.; Domingo, Megan Joyce N.; Fortuna, Edward Paul F.; Garrucho, Geraldine F.; Poral, Mechelle Joy L; Suñer, Ma. Alexis Kleanthe D.; Villanueva, Dana Diane C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)This is a cross-sectional study which aimed to evaluate the knowledge, attitude and practices (KAP) towards pesticide use, its health effects, and the laboratory findings of farmers from Barangay San Ambrosio, Igbaras, Iloilo. Specifically, it aimed to determine the association between the number of years of pesticide use and the farmers’ MCV values. A total of 115 farm workers were interviewed and 109 (94.8%) of them reported pesticide usage. Among farmers who used pesticides, 90 (82.6%) consented to undergo CBC testing. Descriptive statistics was used for socio-demographic profile, KAP, and health effects of pesticide use. Whereas, Chi- square analysis was used to determine the association between pesticide exposure and MCV values. The farmers were knowledgeable about pesticides and its health effects, the route of entry into the body, and the fate of pesticide residues. Despite knowing the adverse effects of pesticides, 107 out of the 109 pesticide-using farmers (98.2 %) still agreed on the use of pesticides, and only 2 (1.8 %) were against it. They justified that pesticides are effective in killing pests to produce abundant crops and only some farmers knew that biological (61.5%) and natural control (63.3%) can be used as alternatives for pest control. In addition, most farmers (n = 94, 86.2%) used PPE when applying pesticides but were inadequate: with 81 (74.3%) using long-sleeved shirts, but only 17 (15.6%) using gloves, and 5 (4.6%) using eyeglasses. The lack of complete and proper PPE may be a factor for the self-reported health effects, such as dizziness (n = 59, 54.1 %), which was the most predominant, followed by burning sensation in eyes/face (n = 54, 49.5 %) and headache (n = 48, 44.0 %). No significant association was found between years of pesticide use and MCV using the chi-square analysis (x2 = 0.583, p = 0.318). It would be useful to minimise the use of pesticides, encourage alternative measures, lessen the time of pesticide spraying, and encourage the use of complete PPE. Prevention and intervention programmes regarding the use of protective measures and monitoring the health status of farm workers should be implemented.Item Project LAGUNDI: Lasapin Ang Ginhawa, URTIs atiN Dapat Iwasan!Acuesta, Doneza Dee L.; Bernaldo, Alyka Joy D.; De la Pena, Leobert Julienne A.; Diestro, Joan Marie T.; Domingo, Megan Joyce N.; Fortuna, Edward Paul F.; Garrucho, Geraldine F.; Poral, Mechelle Joy L.; Suner, Ma. Alexis Kleanthe D.; Villanueva, Dana Diane C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)URTIs are illnesses caused by acute infection of the nose, sinus, pharynx or larynx. It is one of the top three morbidity cases of Brgy. San. Ambrosio, Igbaras, Iloilo. TEAM AMBROSIA’s primary goal was to decrease incidence of URTI among residents. A first medical mission was conducted in order to assess the number of cases, and a second was conducted in order to determine if the intervention has decreased the number of cases. Based on results there was a decrease of 54.29 % in the number of cases, which exceeded the goal of 30%. Based on the pre- and post- test scores, the knowledge on URTI, herbal gardening, smoking improved by 30% or more, meanwhile, the knowledge on waste disposal improved by only 20%. Other activities were also done like mask-making activity, recycling demo, and a URTI-themed community night. Tarpaulins about URTI were given out to each of the 7 sitios while calendars about URTI where distributed to each household. Each sitio had their own communal herbal garden, which includes medicinal plants for the treatment and prevention of URTIs, and other diseases as wells. Residents were taught to make teabags made from the leaves of lagundi, which can be found in their gardens, and can be an alternative source of livelihood for them.Item Project HALTapresyonArbado, Pearl Joy C.; Laurente, Inna Grace R.; Loberiza, Lamont Joseph S.; Militar, Anna Raisa G.; Quidato, Jun Pretzel E.; Ong, Meghan Y.; Salada, Anna Patriz F.; Salcedo, Yehonathan Joseph V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2019-06)The World Health Organization has deemed hypertension to be a global public health issue. In the Philippines, 25% of the adults who are 21 years and above have high blood pressure (2014). HALTapresyon is a project that aimed to prevent and manage hypertension in Barangay Igtalongon, Igbaras, by (1) increasing the knowledge, awareness, and practices (KAP) regarding hypertension by 15%, (2) increasing the KAP regarding physical activity by 15%, (3) increasing the KAP regarding nutrition by 20%, and (4) manage the blood pressure of hypertensive patients. The establishment of a core group and the passing of a resolution were done in order to ensure the sustainability of the project. HyperTEAMsion was also able to donate 6 blood pressure apparatuses for the continuation of the weekly blood pressure monitoring. The project implemented a series of lectures that focused on three aspects: basic knowledge, physical activity, and nutrition, which were identified as factors that greatly affect the development and progression of the disease. This was done to educate the community and promote a healthy diet and active lifestyle as a means of preventing the occurrence and aggravation of hypertension. Activities, such as DASH TAH!, Blood Pressure Moni-training, and the use of a journal, offered a more hands-on approach on educating and improving the practices of the residents. Information, education, and communication materials were also distributed to hypertensives and non-hypertensives alike. Post assessment mean scores for KAP on General Information, Physical Activity, and Nutrition, improved by 16.81%, 15.96%, and 29.70% respectively, all showing a statistical difference. For the non-hypertensives, there was a 36.94% increase in KAP. Weekly monitoring was done in relation to the management of blood pressure and it showed that 77.03% of the hypertensive individuals decreased or maintained their blood pressure. Therefore, Project HALTapresyon could be used on the prevention and management of hypertension.Item Anti-angiogenic properties of Cymbopogon citratus and Moringa oleifera using the Chorioallantoic membrane vascular assayAldeguer, Justine Joy A.; Cañonero, Katrina Anne M.; Cortum, Lyzandra S.; España, Reina Marian E.; Hidrosollo, Manolo Angelo S.; Jaboneta, Abigail Louice D.; Peralta, Karla Danielle T.; Tiosayco, Angeline Veil C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2012-03)In the Philippines, the use of complementary and alternative medicine, such as plants, is beginning to become very common due to the rapidly increasing incidence of cancer in the country. Due to this increase, there have been claims that the most common plants have anticancer properties. These include lemongrass (Cymbopogon citratus) and malunggay (Moringa oleifera). However, there had been scanty literature to prove these claims. The aim of this study is to screen the crude methanol extracts of C. citratus and M. oleifera leaves for anti-angiogenic property using Chorioallantoic Membrane Vascular Assay (CAMVA). Non-lethal concentrations of the plant extracts were embedded into filter paper disks and inserted into the chorioallantoic membrane of the ten-day-old duck embryos. A negative control group of duck eggs were left unopened. After 48 hours of incubation, the number of blood vessels in the chorioallantoic membrane of the duck embryos was manually counted using a dissected microscope. Results showed that extracts of M. oleifera and C. citratus were able to inhibit blood vessel growth in the chorioallantoic membrane of duck embryo. Between the two plants, crude methanol extracts of M. oleifera (12.10+ 0.836) showed a lesser number of developed blood vessels than the crude methanol extracts of C. citratus (14.50+0.637). The anti-angiogenetic properties of these plant extracts could be attributed to the presence of phytochemicals such as citral, quercetin, kaempferol, and niazimicin which have been proven to have anti-cancer properties. In conclusion, M. oleifera and C. citratus have anti-angiogenic properties, hence they are potential sources of natural treatment for tumor.Item Intestinal parasitic infections in children living in selected barangays with urban slums along Iloilo RiverAguro, Eric D.; Banaag, Arlene D.; Jacobe, Venus N.; Ordaniel, Lilac Mared A.; Taleon, Kathleen Joy B.; Yandra, Marnelle Gay A. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2006-03)This cross-sectional study was performed to determine the intestinal parasitic infection in children age 0-13 years old in three selected barangays with urban slums located alongside Iloilo River. A total of 127 stool samples from 65 males and 62 females were examined by utilizing formalin-ether concentration technique (FECT) and direct wet mount thereafter using Lugol's solution. It was found out that 59.8% of the children examined were positive for one or more intestinal parasites. The most frequent soil-transmitted helminth observed was Ascaris lumbricoides (48.0%) followed by Trichuris trichiura (34.6%). Pathogenic protozoans determined were Entamoeba histolytica (9.4%) and Giardia lamblia (0.8%). Other nonpathogenic species identified were Entamoeba coli (15.7%), Blastocystis hominis (3.1%) and lodamoeba butschlii (0.8%). Parasitic infection with single species had the highest frequency of 31.5% compared to multiple infection (28.3%). The relatively high prevalence of these infections among children living three selected barangays with urban slums alongside Iloilo River was found to be independent of factors the respondents’ sex (p=0.106), age group (p=1.691), and consistency of stool sample (p=1.835). Moreover, the degree of infection in terms of sex (p=3.174), age (p=9.136), and consistency of stool samples (p=2.798) were not statistically different from each other. The high prevalence rate of pathogenic intestinal parasitic infection may have also been influenced by factors other than the ones considered, such as environmental and socio-demographic factors. The study highly suggests mass treatment and preventive measures such as health education of the communityItem Effects of crude methanolic extract of purple nutgrass (Cyperus rotundus L.) on the maturation of golfish oocytesAcebuque, Florel May C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)Cyperus rotundus L. is a pernicious weed, which is traditionally used for different medical purposes and is locally recommended for antifertility purposes. A number of pharmacologically important constituents have been previously isolated from the plant. This study aims to test the effects of the crude methanolic extract of C. rotundus L. on the maturation of goldfish oocytes. The extract is found to induce 100% maturation at 5 mg/mL concentration. However, it becomes toxic at higher concentration. All goldfish oocytes (100% ) has died at 20 mg/mL concentration. The steroids and flavonoids present in the plant may be responsible for inducing goldfish oocyte maturation, whereas the alkaloids may be toxic to the oocytes. Further studies using mammalian systems in vivo and characterization of individual constituents are recommended.Item Identification and antibiotic resistance of enterococcus (Schleifer and Kilpper-Bälz 1984) species isolated from Iloilo riverAnsino, Jan Reygine G. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)The presence of Enterococci species in the water has long been regarded as an indicator of fecal contamination. With the increasing rate of antimicrobial resistance globally, infections caused by enterococcal species became difficult to treat. The study determined the species distributions and antimicrobial resistance profiles of Enterococcus species isolated from water and sediment samples in the Iloilo River. Susceptibility of the isolates was tested against ampicillin, penicillin, ciprofloxacin, chloramphenicol, doxycycline, erythromycin, tetracycline, and vancomycin. Among the 115 fecal coliform samples, a total of 61 (53%) isolates were confirmed to belong to the genus Enterococcus. Of these, 40% were identified as Enterococcus faecalis, followed by Enterococcus faecium 33% and Enterococcus avium (4%). Isolates showed resistance to tetracycline (51%), erythromycin (18%), and doxycycline (13%) and a high level susceptibility to ampicillin (100%), chloramphenicol (93%), and penicillin (90%). Multiple antibiotic resistance patterns revealed resistance to at least four antibiotics. Six (12%) vancomycin-resistant Enterococci (VRE) were detected. The results revealed low level of antibiotic resistance in the river. However, the presence of these fecal contaminants in the river can still pose a potential risk to the human health and the of spread of antibiotic resistance genes, indicating the great importance of improving risk management practices in the river.Item Fecundity and egg quality in two species of Siganids (Siganus canaliculatus and Siganus fuscescens) from Lanuza Bay, Surigao del Sur, PhilippinesAnore, Gaguis Anito II T. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2019-06)Siganus canaliculatus and Siganus fuscescens are two of the important yet highly exploited siganid aquatic resources in the Philippines. Only few studies for both species, especially for S. fuscescens, are available in the Philippine setting regarding its fecundity and egg quality. This study was conducted to provide information on the fecundity and egg quality of S. canaliculatus and S. fuscescens from Lanuza Bay, Surigao del Sur, Philippines. Fecundity was determined through gravimetric method in individual gonads. Fecundity range was determined to be 54,856 — 279,957 individual oocytes for S. canaliculatus and 7,397 — 563,722 for S. fuscescens. Fecundities of both species exhibited a weak to strong positive correlation to size, with higher correlation coefficient to standard length for S. canaliculatus and body weight for S. fuscescens. On the other hand, egg quality was determined through mean oocyte diameter, with diameters ranging from 176 — 426 ųm for S. canaliculatus and 171 — 500 pm for S. fuscescens. Mean oocyte diameters of both species exhibited weak positive correlation to size, but with a higher correlation coefficient on standard length. Regulating the capture of smaller-sized fishes and allowing them to grow would promote the increase in fecundity and egg quality of the species.Item Impact analysis of municipal leadership and governance program (MILGP) among municipalities in the province of IloiloArancillo, Ulah Lynn F.; Barcelona, Joser John T.; Cabalo, Jori May G.; Cansancio, Marienne Fay T.; Castillo, Marianne Hillary O.; Nufuar, Isa Adelie S.; Palanog, Riananess Q.; Pido, Theo Joseph D.; Repospolo, Melvie Dale P. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2019-06)The Zuellig Family Foundation (ZFF) and Department of Health initiated the Municipal Leadership and Governance Program (MLGP), a two-cycle program that aims to guide the Filipinos in achieving health equity and resilience through mentoring and training teams led by local health leaders to improve the six building blocks of health. It also aims to achieve the Sustainable Development Goals through ensuring that municipal health systems be responsive, equitable, and sustainable. This research aims to assess the municipality performance and health outcomes to measure the success of the MLGP intervention. Secondary data representing health outcomes from MLGP and non-MLGP municipalities and consolidated roadmaps were gathered and statistical analysis was performed using IBM SPSS Statistics 23. Among the health indicators that were used to evaluate the program under Cycle 1, SBA and FIC showed a statistical significance while the remaining indicators, MMR, IMR, CPR, and FBD did not. All six health outcomes for Cycle 2 municipalities did not show any statistical significance from 2015-2018. Statistically significant improvements were observed on Health Human Resource on Modules 2 and 3, and all the modules in Leadership and Governance of the MLGP Cycle 2 while the other five building blocks did not show statistical significance. Changes in the health outcomes under MLGP Cycle 2 and non-MLGP municipalities showed that they have improved in terms SBA and FBD only. Although significant relationships were found between municipality performance and the health outcome indicators, it must be noted that there are confounding factors not accounted which may have interfered with the results and findings of this study.