Bacaling, Marivic T. (Division of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Visayas, 2005-04)
The cytotoxicity test of crude methanolic leaf extract using Brine Shrimp Lethality Assay revealed that Alibutbut (Tabernaemontana pandacaqui Poir.) is safe to use in treating vibrio-infected penaeid shrimps.
The effectiveness of the alibutbut powder against vibriosis was examined through the determination of the post-larvae 20 (PL 20) penaeid shrimp mortality and the water microbial assay after 48 hours. Percent penaied shrimp mortality (0-6.67%) and microbial count (2.91 x 10^4- 3.26 x 104 cfu/mL) of the plant treatments were significantly lower compared to the negative control (no treatment) that exhibited 50% penaeid shrimp mortality and a microbial count greater than 6.00 x 10^4 cfu/mL.
The results of the experiment showed that Alibutbut plant powder is indeed effective in controlling Vibrio harveyi bacteria.
