UPV Theses and Dissertations
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Item Household food security and nutritional status of preschoolers in Valderrama, AntiqueBraga, Mayet Ann D.; Gordon, Cyrus John G.; Guisihan, Thanz Pamela G., II; Jamili, JC John T.; Ledesma, Migil G.; Lim, Diana L.; Lim, Lenny Joy G.; Malalu-an, Rosalie A. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2005-03)The study is a cross-sectional survey of 138 randomly selected households from ten (10) similarly randomly-selected barangays in Valderrama, Antique. This study aims to determine household food security and nutritional status of pre-schoolers in Valderrama, Antique. The study specifically aimed to: determine the status of household food security in Valderrama, Antique; describe the characteristics of households in terms of Age and Highest Educational Attainment of Caretakers, Annual Family Income (in Pesos), Income Allotment for Food, House Ownership, Number of Household Members and Food Source; and compare the nutritional status of pre-schoolers of food secure and food insecure households based on the following anthropometric parameters: Weight for age (WFA), Height / length for age (H/LFA) and Weight for height (WFH). The study is patterned after the national survey done by the Food and Nutrition Research Institute (FNRI) that ranked Antique as the province with the highest degree of malnutrition. Food security was determined using the Food-Security/Hunger Core Module, 3-Stage Design, with Screeners. Anthropometric measurements were taken from randomly selected preschoolers aged 0-5 from each household to determine the nutritional status. The current study revealed that the prevalence of food insecurity in Valderrama, Antique is very high at 94.9%. The level of food insecurity was further determined and the following results were obtained: 17.6% of the food insecure households were without hunger; 56.5% were the food insecure households were without hunger; 56.5% were food insecure with moderate hunger, and, 26.0% were food insecure with severe hunger. Only 5.1% of the households surveyed were food secure. The results showed that in terms of the anthropometric parameters used in the study (Weight for Age, Height for Age, Weight for Height), all pre-schoolers in food secure households were normal. On the other hand, in food insecure households, the prevalence of underweight, stunting and wasting were high. Using the parameter Weight-For-Age (WFA), the prevalence of underweight children was 38.2%, which is higher than the national average of 27.6%. Using the Height-for-Age (HFA), 43.5% of pre-schoolers in the area were stunted (underheight) and which is again higher than the national average of 38.5%. The prevalence of wasting in the study was alarmingly high (14.5%) which is almost three times higher than the national average of 5.5%. The results obtained may be used as baseline data in planning intervention programs by the municipal government and concerned agencies (Government Organizations and Non-Government Organizations) to address the alarming problem of malnutrition in the municipality.Item Comparison of direct microscopy and concentration techniques using household bleach for the detection of acid-fast bacilli in sputumBernabe, John Marvin S.; Biaoco, Beverly G.; Castillanes, Melvic A.; Fernandez, Ia Jean F.; Geraldoy, Ruffa V.; Molina, Ryge Albert D.; Pluma, Zyra April D.; Que, Agnes Karen B.; Servidad, Mercy Jeane C.; Tamayo, Kyria Mae Marre P. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2008-03-29)Several published studies have demonstrated that the sedimentation and centrifugation concentration techniques which utilize sodium hypochlorite are more efficient in detecting acid fast bacilli in sputum smears compared to the conventional direct smear method. This study was conducted to (1) compare the proportion of AFB-positive samples by direct microscopy with the household bleach centrifugation concentration technique; (2) compare the proportion of AFB-positive samples by direct microscopy with the household bleach sedimentation concentration technique;(3) compare the proportion of AFB-positive samples by household bleach sedimentation with the centrifugation concentration techniques; and (4) compare the cost of the direct smear method, centrifugation concentration technique, and sedimentation concentration technique for detection of AFB. Sputum specimens were collected from TB-DOTS clinics in Miag-ao and San Joaquin, Iloilo and from Medicus laboratories in Iloilo City. The specimens were then processed through the direct smear method, centrifugation concentration method, and sedimentation concentration method. Out of 174 sputum samples included in this study, results revealed that (1) there was no significant difference in the proportions of AFB-positive slides detected using the direct microscopy and centrifugation concentration technique; (2) there was no significant difference in the proportions of AFB-positive slides detected using the direct microscopy and sedimentation concentration technique; (3) there was no significant difference in the proportions of AFB-positive slides detected using the centrifugation and the sedimentation concentration techniques; and (4) simple cost analysis of the three methods suggests that the direct smear method is the most cost efficient. The findings indicate that whichever method is used would yield almost the same proportions of AFB-positive slides. Internal quality control should be maintained throughout the laboratory work to obtain reliable results, as it is the manner of processing that determines the outcome of this study and its validity.Item Ascariasis and protein energy malnutrition among schoolchildren of Monteclaro Elementary School, Miag-ao, IloiloBerlin, Nellisa Marie; Chua, Enrique III; Daquilenea, Miko; Dorde, Cristy Joy; Hosillos, Juls; Libo-on, Dyann Dolour; Panizales, Katherine Royce; Robleza, Xela Patricia; Sanchez, Maria Lourdes; Sumayo, Marilyn (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2008-04)Ascaris infections lead to malnutrition and poor cognitive development especially to children aged 5-15 years of age. In the Philippines, protein-energy malnutrition is one of the ten leading causes of child mortality. From January to February 2008, a cross- sectional descriptive study was carried out in Monteclaro Elementary School to describe the prevalence of ascariasis and the prevalence of protein energy malnutrition and relate it to the demographic factors such as age group, educational attainment of the mother, annual family income, availability of toilet, and proper hand washing practice. Sixty eight pupils of the said school were included in the study with ages ranging from 6-13 years. Fecal sample of the participants were collected, processed using the Kato-Katz smear technique, and microscopically examined to determine the prevalence of infections. The weight was measured for weight for age index. A self administered questionnaire was given to know about socio-economic factors. Handwashing activities were utilized as a basis for personal hygiene. The result of the study shows that 12% of the study population has a mild A. lumbricoides infection. Weight for age measurement shows that 21% of the pupils are underweight. Pupils of the age group 12-14 were of the highest frequency of ascaris infection and pupils of the age group of 6-8 were of the lowest frequency. Twelve percent of pupils who have toilets in their household are positive for ascaris infection. All pupils failed in the handwashing activity. Twenty seven percent of the pupils whose mothers have no formal education were underweight. This is due to the level of education that the mother has achieved. Mothers who have undergone tertiary education know more about the proper ways to nourish their children thereby minimizing children who are underweight. Twenty-three percent of the pupils included in the study were below normal in the weight for age index. Trend shows that those families with lower income have a higher frequency of respondents that are underweight. For pupils with below normal weight for age 50% are without toilet while 19% with toilet. There is low prevalence of ascariasis in Monteclaro Elementary School. The results of this study do not truly show the relationship of ascariasis with protein energy malnutrition. Inconsistencies of the results with the findings of other studies are possibly due to low number of positive ascaris-infected participants and the low number of overall participants. Increase in the sample size and ascariasis positive participants within the sample are recommended to get more accurate results.Item Acceptability of the newly-formulated food recipes among selected age groups at Brgy. Tabat Tubungan, IloiloBeluso, Heziel F.; Buaron, Michelle Jane A.; Hubag, Angelie Rose C.; Macahilo, Judeilan A.; Mationg, Mary Lorraine S.; Nismal, Myles C.; Pido,Dianah D.; Simaurio, Geraldine C.; Sodusta, Valerie Joy M.; Yasa, Kutch V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2007-03)This research study is a cross-sectional survey done among 90 conveniently selected residents of Brgy. Tabat Tubungan, Iloilo in order to determine the acceptability of the newly-formulated food recipes based on sensory analysis (appearance, smell, taste and texture), general acceptability, cost, and food action response. The ingredients used in each of the three new recipes namely tortang malunggay, alugbati-squash ukoy and Palawan delight are nutritious, cheap and indigenous to the community. The acceptability test was done among the three selected age groups: school-age children (6-12 yrs old), adolescents (13-19 yrs old) and adult (20-40 yrs old). Pre-tested structured questionnaires were used in the evaluation of the acceptability of the three recipes. Results were analyzed using frequency distribution and test of proportion to determine whether or not the prepared recipes are acceptable. Chi-square analysis was used to test the independence of acceptability of the food items based on the set criteria and the age group categories of the panelists. The results show that three newly formulated recipes namely tortang malunggay, alugbati-squash ukoy and Palawan delight were found to contain high amount of energy content (107 kcal, 181 kcal and 160 kcal) and essential nutrients such as vitamins A and C, calcium and iron which are needed and vital to the body. The percent Recommended Dietary Allowance requirements derived from the three newly formulated food recipes were 5.35% of the total caloric need per serving for the tortang malunggay, alugbati-squash ukoy and palawan delight could supply 9.05% and 8.00% of the total caloric needs. For the sensory test, results show that in terms of the appearance, the three recipes: tortang malunggay, alugbati-squash ukoy and Palawan delight are acceptable to 82.22%, 95.56%, and 84.44% of the panelists respectively. Smell of the three recipes is acceptable to 87.78% of the panelists. Among the ninety panelists, 87.78% and 83.33% said that the taste of the food recipes is acceptable. In terms of texture, tortang malunggay received the highest acceptance level of 88.89%. Based on general acceptability, Palawan delight was liked by 93.33% panelist. The computed Chi-square values show that the acceptability based on sensory analysis and general acceptability of the three food recipes (except the taste of alugbati-squash ukoy) is not dependent on the age group category of the panelists. The costs of the three recipes were also acceptable to 95.56% of the panelists. As to the food action-response, 95.56% expressed a favorable response. Because of the positive results obtained during the acceptability test on the three food items, the researchers recommend these recipes be included in Food Supplementation and Nutrition Education activities.Item Bacteriological survey and detection of Escherichia coli, Salmonella spp., Streptococcus spp., and Vibrio spp. in bottom sediments from two sites in Iloilo RiverBarrera, Christopher (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2010-04)Bacterial contamination in the Iloilo river occurs because of various human activities. The assessment of the river was done using microbiological parameters (HPC, TC, FC, and FS) for 7 months in Sooc Bridge and Carpenter’s Bridge using bottom sediments for samples. Sediment was used because bacteria accumulate over time in the sediment due to high amounts of organic and inorganic matter in the substrate and this leads to better survivability of bacteria. The values obtained from microbiological parameters investigated are generally higher in Sooc Bridge. HPC results show that the higher mean value was in Sooc Bridge(7.43 x 108 CFU/lOOg). The levels of TC on both sampling sites ranged from 9.5 x 102to 2.40 x 104 CFU/100g. For TC, Sooc has the higher mean (1.76 x 104 CFU/lOOg) for 7 months. FC values ranged from 3.0 x 101 to 2.4 x 104 CFU/lOOg. Sooc bridge has the higher mean(1.17 x 104 CFU/lOOg) between the 2 sites. Lastly, the FS value range from 2.9 x 103 to 2.4 x 104 CFU/lOOg. Also, Sooc Bridge has the higher mean value (1.58 x 104 CFU/lOOg). There are no significant differences on the microbiological parameters used in each site. The FC/FS ratio revealed that mixed fecal materials contaminate the river. Among the four pathogenic bacteria detected, Salmonella spp. was the most abundant for 7 months (63-65%) and E. coli was the least abundant(>l%). Based on the criteria on sediment quality evaluation, there is little to moderate loading of organic and inorganic matter.Item Gross alpha and gross beta radioactivity and heavy metal concentration profiles of well water of the Calahunan dump site, Mandurriao, Iloilo CityAmarante, Hena Charma; Basquez, Mark Anthony; Brunio, Christian Allan; Dable, Nesel; De la Pena, Pamela-Di; Genterola, Janis; Oredina, Josette Marie; Padayhag, Dave (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2005-03)The study aimed to establish presence of radioactivity and concentration of heavy metals specifically lead and chromium in drinking water from wells within one-kilometer radius from the approximated center of the Calahunan dump site. Results showed that four out of twelve wells have positive results fro gross alpha activity and gross beta activity: wells 2A, 2C, 3A, and 3D. Well 2A, 2C and 3D registered alpha activities of 0.54, 0.033 and 0.369 Bq/L respectively while Well 3A registered beta activity of 1.0 Bq/L. Wells 2A and 3D exceeded the radiological limits set by WHO and PNSDW of 0.1 Bq/L for alpha activity and 1.0 Bq/L for beta activity. These wells that are found adjacent to the perimeter fence of the dump site registered alpha activity (wells 2A, 2C and 3D) and beta activity (well 3A). Radiological activities in all other wells sampled registered less than lower limit of detection. For gamma activity, all wells recorded 137- Cs and 134-Cs activity less than the lower limit of detection. For chromium and lead concentration using FAAS, only well 3C has a chromium concentration of 0.0952 ppm and this exceeded the PNSDW standard for chromium in drinking water (0.05 ppm) FAAS has not recorded any value for lead. For chemical constituent analysis using XRF, two wells in zone 1 (LA and IC), have recorded lead concentration of 0.9 and 0.136 ppm, respectively. In zone two, only well 2D registered a lead concentration of 0.248 ppm. In zone 3, well 3C registered a lead concentration of 0.397 ppm. For chromium concentration, only well 2A in zone 2 has a chromium concentration of 1.79 ppm. The spot interview and ocular inspection found out that the following garbage are disposed of indiscriminately tn the dump site: (1) Television picture tubes, (2) Dysfunctional Computer Monitors, (3) Used syringes and needles, (4) Hospital wastes, (5) Automobile batteries and dry cells, (6) scrap iron, tin cans, paint cans, paint cans, etc., (7) polyethylene bottle, glass bottles, and containers of similar types, and (8) other organic and inorganic wastes. Wells whose values for gross alpha, lead, and chromium concentration exceeded the standard are unsuitable for dinking and other domestic uses. The observation that wells (2A and 3D) near the Calahunan dump site registered higher-than-standard alpha activity may be a good basis to conduct further research particularly on the effect of leachate from dump site.Item Physico-chemical analysis and assessment of river waters discharged by sugar milling companies in Negros OccidentalAlger, Niña Xiamima A.; Arinzol, Shaira S.; Bobon, Steve Paolo P.; Daquilanea, Mikkaela C.; de Leon, Sarah C.; Hilado, Paolo C.; Lastica, Ariel C.; Manderico, Regine Viel D. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2010-03)This cross-sectional study was focused on the river waters discharged by six sugar milling companies in Negros Occidental. These companies utilize river water in order to operate and may alter the characteristics of the waters. This study aims to analyze and assess the physical characteristics, namely pH, oil and grease, temperature, salinity, turbidity, and color, and the chemical characteristics, namely biological oxygen demand (BOD), dissolved oxygen (DO), ammonia, phosphorus, and lead content, of these waters in order to identify the alterations upon its usage, detect abnormal characteristics, and eventually determine possible environmental and health hazards. Three samplings were done for each river’s downstream and upstream portions. The following tests and instruments were performed and were used on the samples: pH Meter for pH, DO Meter for DO, Wrinkler’s Test for BOD, PE Ascorbic Acid Test for phosphorus, Spectrophotometry for turbidity, Phenate Test for Ammonia, Hand Refractometer for salinity, Visual Comparison for color, Digital Thermometer for temperature, Flame Atomic Absorption Spectrophotometry for lead content, and Petroleum Ether Extraction Method for oil and grease. It was discovered most of the discharged river waters apparently had decreased pH and DO and increased BOD, temperature, salinity, ammonia, phosphorus, turbidity, and oil and grease content. Lead was detected on the all of the downstream portions. The pH and DO of most of the discharged river waters were ostensibly below the acceptable values while the oil and grease, ammonia, phosphorus, salinity, and lead content were above the acceptable ones. Temperature, color, and turbidity were the only parameters which fell within the acceptable values. Therefore, there can seemingly be environmental and health hazards posed on the residents living downstream since they are having direct or indirect contact with the contaminated or polluted water. It is recommended that laws should be made with regards to how the residents use the water and that the implementation of existing laws should be strengthened. Lastly, it is strongly advised that the DENR should increase the number of parameters analyzed during their regular monitoring, that the SMCs should improve the treatment of the waters, and that the effects of farmlands and communities on the river should be identified.Item Microbiological water analysis of selected point sources in Iloilo CityAlama, Jannie Mae G.; Calitis, Ma. Socorro Fatima P.; Cervantes, Ria Angela E.; Garcia, Marian Aisa C.; Otico, Pelita A.; Pacheco, Donna Ginn G.; Taquiso, Jezreel l. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2006-04)The recreational use of the Iloilo River according to the Iloilo River Master Plan entails various studies to support it being safe for swimming and other activities. The Department of Environment and Natural Resources (DENR) Region VI has done very few studies to determine this particular aspect of the Iloilo River. Thus, the present study was conducted to determine the bacteriologic content of water in selected point sources in Iloilo River, Iloilo City and the possible correlations to the incidence of health risks. Specifically the study aims to: (1) determine significant differences in the physico-chemical characteristics of water samples from each point source; (2) to determine the total and fecal coliform counts number for each water sample from the selected point sources; (2) detect the presence or absence of E. coli, Salmonella spp., and culturable Vibrios in water in selected point sources; (3) describe the incidence of health risks; and (4) correlate the heterotrophic plate count for total coliforms, Most Probable Number for Fecal Coliforms and the number of symptomatic respondents. The results of the study showed that the total coliform count for Point source A (HPC=6000/l00 ml) is higher compared to the standard set by the DENR (5000/100 ml) while Point source B and C are within the range. The concentrations of E. coli, in water samples A, B, and C (2400 MPN/100 ml sample) were found to be higher than the DENR standard for fecal coliforms for Class C Waters - fresh water, (200 MPN/lOOml sample). Although results indicate that the river water has high fecal contamination, there were no significant correlations between heterotrophic plate counts, Most Probable Number for Fecal Coliforms and the number of symptomatic respondents for each point source.Item Antibiotic resistance patterns of selected pathogenic bacteria isolated from water and bottom sediments in the Iloilo tidal inlet (Iloilo River)Agustin, Eda Mae S.; Cabrias, Ligaya April May S.; Fuentebella, Elaine Joyce V.; Gestuveo, Rommel J.; Lagon, Raymond Anthonn Kriztee J.; Saldajeno, Cypruz Anne L.; Tunguia, Saer Martin J.; Villanueva, Regene Patricia Natividad V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2010-03)In recent years, antibiotic resistance of pathogenic bacteria has become a public health concern. Although little is known of its environmental implications the presence of both potentially pathogenic bacteria and fecal coliform in rivers and other bodies of water raises the question of whether resistance transfer may actually occur in bodies of water. In relation, the Iloilo River for one has experienced changes in both ecological and sanitary conditions. With this threatening problem of both ecological and fecal pollution, the river could harbor microflora that could be of public health concern. Using the Kirby-Bauer Method, this study generally aims to determine the antibiotic resistance of pathogenic bacteria isolates (E. coli, S. typhi and V. cholerae) from water and bottom sediment samples from selected sampling areas in Iloilo River, Iloilo City. Stock cultures of bacterial isolates were obtained from the UPV-National Institute of Molecular Biology and Biotechnology (UPV-NIMBB) and were subjected to antibiotic resistance analysis using 12 different antibiotic classes. Results showed varying degrees of resistance in all of the sampling sites and species. Most isolates were highly resistant to penicillin and oxacillin and least resistant to ciprofloxacin. Multidrug-resistant isolates were also observed in all of the sampling sites and species. More isolates from bottom sediments were observed to be highly resistant than the isolates from water samples. Pearson correlation revealed that the antibiotic resistance patterns among the different species isolated from the different sources and sampling sites appeared to vary in a systematic manner indicative of a similar mechanism of antibiotic resistance development.