UPV Theses and Dissertations
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Item Antibacterial activity of aqueous and methanol extracts of selected basidiomycetes from UPV, Miagao, Iloilo PhilippinesAmbubuyog, Marja Victoria U. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2013-03)The Basidiomycetes represent an enormous source for natural products with diverse chemical structures and wideranging antimicrobial activity. This study was done to investigate the antibacterial property of selected species of basidiomycetes aqueous and methanolic extracts against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Specifically, it aimed to (1) determine the antibacterial activity of the aqueous extract of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa based on the zone of inhibition; (2) determine the antibacterial activity of the methanolic extract of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa based on the zone of inhibition; and (3) compare the aqueous and methanolic extracts of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa based on the zone of inhibition. A total number of ten species were collected namely Auricularia sp., Schizophyllum sp., Trametes pubescens, Trametes versicolor, Trametes elegans, Ganoderma sp.(l), Ganoderma sp.(2), Stereum sp. and two unknowns. Dried samples were crushed and extracted with water and methanol. Antibacterial activity was determined using the Kirby-Bauer disc diffusion method. The results showed a negative to weak inhibition against the test microorganisms which is inconsistent with the results of previous studies. It could possibly be attributed to the preparation of the samples and the fruiting body maturity that influenced the antibacterial potential of the samples. It appears that fruiting bodies used in the present study may not be reliable sources of bioactive compounds for antibacterial activity.Item Effects of crude methanolic extract of purple nutgrass (Cyperus rotundus L.) on the maturation of golfish oocytesAcebuque, Florel May C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)Cyperus rotundus L. is a pernicious weed, which is traditionally used for different medical purposes and is locally recommended for antifertility purposes. A number of pharmacologically important constituents have been previously isolated from the plant. This study aims to test the effects of the crude methanolic extract of C. rotundus L. on the maturation of goldfish oocytes. The extract is found to induce 100% maturation at 5 mg/mL concentration. However, it becomes toxic at higher concentration. All goldfish oocytes (100% ) has died at 20 mg/mL concentration. The steroids and flavonoids present in the plant may be responsible for inducing goldfish oocyte maturation, whereas the alkaloids may be toxic to the oocytes. Further studies using mammalian systems in vivo and characterization of individual constituents are recommended.