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    Filtration rate of the marine clam, Kaltelysia sp. at different light intensities and fed with microalgae Isochrysis galbana and Chaetoceros calcitrans
    Bravo, Agnes Therese A. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2005-04)
    Filtration rate of the marine clam, Katelysia with body lengths ranging from 5-6 cm and body weight from 45-65 g were determined by using the indirect method of suspension depletion and monitored by direct algal cell count. The effects of light intensity and algal species on the feeding behavior of the clam were determined. The first treatment involves total darkness where the basins containing the clams were covered with black plastic bag. In the second treatment, the basins with the clams were placed under ordinary light condition (12h light; 12h dark) and in the third treatment, the basins with the clams were positioned under a 20-watt bulb with continuous illumination (24h light). Results showed significant differences in the filtration rates of clams in different light intensities. It showed higher filtration rate in the total darkness treatment (F=0.132555907) followed by the 12h light: 12h dark treatment and lowest at the treatment provided with 24 h illumination. Apparently, illumination affected valve opening and siphon extension of the clam which also affected the filtration rate of Katelysia. Under total darkness treatment, the clams opened their valves and extended their siphons. Hence, there was highest ingestion of algae or filtration in total darkness which showed to be the optimum condition to stimulate or promote feeding. In the algal species preference experiment, the food used were either Isochrysis galbana, Chaetoceros calcitrans , or a mixture of both. Results showed that I. galbana had the highest number of cells ingested by the clams and removed from suspension. This is considered to be the preferred algal species (F=0.119713) followed by a mixture of both, and lowest on the treatment where C. calcitrans was used as food. The preference of the clam on Isochrysis galbana over Chaetoceros calcitrans could be its smaller size based on dry weight.
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    Identification of intertidal fungi from mangroves in Iloilo River
    Bonifacio, Jo F. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2002-04)
    This study aims to describe, identify and classify intertidal fungi through direct examination of the collected mangrove wood samples in Iloilo River, Iloilo City, Philippines. This will provide additional information about the occurrence of fungi in estuarine environments. One hundred samples of decayed but attached roots and stems of unidentified mangroves were collected in each of the intertidal zone of the three study sites (Study Site I: Sooc, Oton, Iloilo; Study Site II: Dulonan, Arevalo, Iloilo City; Study Site III: Diversion River, near Marina Restaurant) in Iloilo River. In Study Site 1, The total number of species collected was 11, 4 of which belong to Phylum Ascomycota, 2 belong to Phylum Deuteromycota and 5 belong to Phylum Basidiomycota. In Study Site II, there were 10, 6 belong to Phylum Ascomycota, 3 to Phylum Deuteromycota and 1 to Phylum Basidiomycota and in Study Site III, total number of species was 14, 7 belong to Phylum Ascomycota, 4 to Phylum Deuteromycota and 3 to Phylum Basidiomycota. The total number of species of fungi collected in the three study sites were 20 (8 ascomycetes, 7 basidiomycetes, and 5 deuteromycetes). The frequently occurring fungi (10-29 % fiequency of occurrence) are Eutypa sp. (15.3%), followed by Periconia prolifica (13 %) and Verruculina enalia (10.3%). The remaining 17 species were collected infrequently (<10 % fiequency of occurrence). The ascomycetes were the most dominant group of fungi collected in this study.
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    Removal of Harmful Algal Bloom (HAB)-forming organisms using ball clay: Factors and effects of clay addition
    Rivera, Peter Paolo L. (Marine Science Institute, College of Science, University of the Philippines Visayas, 2015-05)
    Global concern for Harmful Algal Blooms (HABs) has increased due to their frequency of occurrence and associated negative health and economic effects. Control measures for HABs/red tides have been developed to alleviate their effects in the marine and coastal environments. The use of clays and modified sands/sediments to remove/control HABs is one of the promising and attractive direct control options for areas with persistent HAB problems. However, factors such as pH and ionic strength and mechanisms and kinetics of algal removal through clay addition/spreading are not fully understood. This study examined the effect of different pH levels and varying ionic strength on the removal efficiency (RE) of ball clay on toxic dinoflagellates: Pyrodinium bahamense var. compression, Gymnodinium catenation, and Alexandrium tamarense complex. High REs by ball clay were observed on Pyrodinium and Gymnodinium. On the other hand, only minimal removal was observed with Alexandrium. This implies that ball clay has a varying affinity towards different HAB species. Removal was also found to be highly dependent on bloom stages and varies for a given target species. RE of ball clay for the three test organisms increases as the pH of the solution decreases (83.1% for Pyrodinium, 81.2% for Gymnodinium, 89.4% for Alexandrium at pH 6. Results from this study show that with increasing salinity and/or ionic strength, RE also increases (82.7% for Pyrodinium, 88% for Gymnodinium, and 79.5% for Alexandrium at ionic strength of 1.0). Results show that changing the pH and ionic strength influenced the zeta potential (electrokinetic potential) of both the toxic algal cells and ball clay, hence affecting algal flocculation and leading to the effective removal of the algal cells. Microalgal cells are negatively charged in growth media at pH values (7-9 for most algal species) typical for growth and the charges cause adjacent cells to repel each other and remain suspended in the medium. The negative charges apparently derived from the water and dissociation of functional groups on algal cell walls, and vary among different algal species. The changes in pH influence the degree of surface dissociation, thus affecting the cell surface charge. The zeta potential of ball clay as a function of varying ionic strength and salt concentrations showed that zeta potential decreases (more negative) with increasing ionic strength from 0 to 1 and/or salinity from 0 to 36 ppt (in contrast to algal cells). When the ionic strength of the solution is increased, the charge potential difference between clay particles and algal cells also increases, thereby increasing the affinity between the two particles. Cell death and lysis were evident within the suspended and settled clay matrix from direct microscopic observation. The cells appeared moribund, and stained cytoplasmic material could be seen leaking into the medium. Cell death may have been due to the direct physical contact between the cells and clay particles. Cell lysis occurred over a 1–2-hour period in single-species cultures of HAB organisms. Measurement of fluorescence properties through confocal laser scanning microscopy (CLSM) of microalgae has proven that a rapid and sensitive ecotoxicity method could be developed where the motility and viability of cells flocculated with clay could be examined. The substantial release of the nucleotides and intracellular materials from the algal cells indicates the expulsion of the thecal plates and cell membrane leading to the disintegration of the algal cells upon clay addition. Cellular damage was further confirmed by metabolic imaging through an esterase activity assay. Esterase activity in control and treated cells was studied by fluorescence imaging after staining with fluorescence diacetate (FDA), a technique to probe cell membrane integrity and cell viability. FDA-positive cells decreased through time until 24-hour upon exposure to ball clay. Future studies are needed to investigate the actual mechanism of how clay induces cell lysis or mortality. Cell mortality may be due to natural senescence or programmed cell death (PCD) under stress-promoting conditions and not related to any chemical or physical interaction between the clay and the algal cells. It is also interesting to look at the possible formation of ecdysal and temporary pellicle cysts upon clay treatment. The possible formation of temporary cysts by HAB cells may play a role in bloom regrowth. The encystment and excystment may enable HAB cells to escape entrapment, allowing a bloom to reform.