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    Effects of crude methanolic extract of purple nutgrass (Cyperus rotundus L.) on the maturation of golfish oocytes
    Acebuque, Florel May C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)
    Cyperus rotundus L. is a pernicious weed, which is traditionally used for different medical purposes and is locally recommended for antifertility purposes. A number of pharmacologically important constituents have been previously isolated from the plant. This study aims to test the effects of the crude methanolic extract of C. rotundus L. on the maturation of goldfish oocytes. The extract is found to induce 100% maturation at 5 mg/mL concentration. However, it becomes toxic at higher concentration. All goldfish oocytes (100% ) has died at 20 mg/mL concentration. The steroids and flavonoids present in the plant may be responsible for inducing goldfish oocyte maturation, whereas the alkaloids may be toxic to the oocytes. Further studies using mammalian systems in vivo and characterization of individual constituents are recommended.
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    Survival of nursery-reared juvenile milkfish, Chanos-chanos at different transport density, temperature and anaesthetic combinations
    Failaman, Alan N. (University of the Philippines Visayas, 2020-06)
    ABSTRACT Experiments were conducted to optimize transport conditions of nursery cultured juvenile milkfish in a closed plastic bag based transport system. In the first experiment combined effects of temperature (25-27°C, 20-22°C and 15-17°C) and anesthetics (2-phenoxyethanol at 200 mgL-1 for initial sedation and 80 mgL-1 for transport sedation) on oxygen consumption and survival ofjuvenile milkfish, Chanos chanos, were evaluated. Another experiment was conducted to optimize transport conditions at fish densities of 200, 400, 600, 800 and 1000 gL-1 for 4, 8, 12,16 and 20 hour duration. The study showed no significant difference on oxygen consumption rates ofmilkfishjuveniles subjected to various water temperature range and anesthetic combinations. Temperature range of 20 to 22°C in combination with anaesthetic (2-phenoxyethanol) application was the best condition for long duration confinement of milkfish juvenile in closed plastic transport bags. The study established the optimum juvenile milkfish transport density and the effective transport duration. With anaesthetic application (2-phenoxyethanol), juvenile milkfish density of 600 gL-1 is effective for 20 hours oftransport duration, 800 gL-1 for 8 hours oftransport duration and 1000 gL-1 effective for 4 hours transport duration. These results provide a practical protocol for the transport of juvenile milkfish from the nursery culture area to the grow-out production area.