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UPV Theses and Dissertations

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    Anti-microbial activity of aqueous extracts from selected Philippine soft coral species
    Aguilar, Remotito A. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1994-03)
    Five species of soft corals were collected from Taklong Islands Guimaras and then extracted with water. The aqueous extract was assayed for anti- microbial activity. Potent extracts were tested for its minimal inhibitory concentration against the most sensitive microorganisms. The equivalent potency was also determined using PEN VK as the standard antibiotic. Lobophyton was found to inhibit S. aureus at 50% concentration of the aqueous extract (ATCC 25923) while Sinuloria. sp. was found to inhibit the growth of both S. aureus (ATCC 25923) and B. subtilis (NSRI-1). Aqueous extracts of Lobophyton sp.has an equipotency of 6.64 units/ml of PEN VK against S. aureus, whereas aqueous extracts of Sinulcria sp. has a potency of 104 units/ml of PEN VK against B. subtilis.
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    Antibacterial activity of Aqueous and Methanol extracts of selected Basidiomycetes from UPV, Miagao, Iloilo, Philippines
    Ambubuyog, Marja Victoria U. (Division of Biological Science, College of Arts and Sciences, University of the Philippines, 2013-03)
    The Basidiomycetes represent an enormous source for natural products with diverse chemical structures and wide-ranging antimicrobial activity. This study was done to investigate the antibacterial property of selected species of basidiomycetes aqueous and methanolic extracts against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Specifically, it aimed to (1) determine the antibacterial activity of the aqueous extract of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa based on the zone of inhibition; (2) determine the antibacterial activity of the methanolic extract of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa based on the zone of inhibition; and (3) compare the aqueous and methanolic extracts of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa based on the zone of inhibition. A total number of ten species were collected namely Auricularia sp., Schizophyllum sp., Trametes pubescens, Trametes versicolor, Trametes elegans, Ganoderma sp.(l), Ganoderma sp.(2), Stereum sp. and two unknowns. Dried samples were crushed and extracted with water and methanol. Antibacterial activity was determined using the Kirby-Bauer disc diffusion method. The results showed a negative to weak inhibition against the test microorganisms which is inconsistent with the results of previous studies. It could possibly be attributed to the preparation of the samples and the fruiting body maturity that influenced the antibacterial potential of the samples. It appears that fruiting bodies used in the present study may not be reliable sources of bioactive compounds for antibacterial activity.