UPV Theses and Dissertations
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Item Microbiological assessment and detection of potentially pathogenic bacteria on three areas along the Iloilo RiverCaballo, John Carlo P. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2011-04)The Iloilo River, which traverses the whole length of Iloilo City, serves as a water source for different human activities hence, subjecting it to a variety of pollutants. Microbial and physico-chemical parameters of the three areas along the Iloilo River (Carpenter’s Bridge, IBRD Bridge, and Dungon Creek) were assessed to in order to provide a quantifiable evaluation of the river’s water quality. The presence of Salmonella spp., Eschericia coli, Streptococcus spp. and Vibrio spp., was also determined. HPC counts indicate very-high to extremely-high levels of bacterial loading (5.31 x 105-l .60 x 106 CFU/lOOmL) among the three sampling areas. Total coliform levels indicated contamination of the river from outside sources, potentially from domestic sewage discharges and run-offs from nearby establishments. The FC: FS ratio suggests contamination mostly came from mixed origins, with the noted exception of Carpenter’s Bridge, which is dominated by human fecal contamination. Minimal variations in physico-chemical parameters were observed for all areas. Significant correlations were found among salinity (p=0.002) and temperature (p=0.042) with TC; between FS with dissolved oxygen (p=0.00), and between FC and temperature (p=0.005). Contamination of the river by organic and fecal matter from animal and human sources provided a suitable habitat for pathogenic bacteria. A considerable quantity of potentially pathogenic bacteria such as; E.coli, Salmonella spp., Streptococcus spp. and Vibrio spp., were isolated from each sampling area. Exposure to these pathogens in drinking and recreational waters poses health risks to local inhabitants and to any individual who would consume water from the riverItem Bacteriological survey and detection of Escherichia coli, Salmonella spp., Streptococcus spp., and Vibrio spp. in bottom sediments from two sites in Iloilo RiverBarrera, Christopher (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2010-04)Bacterial contamination in the Iloilo river occurs because of various human activities. The assessment of the river was done using microbiological parameters (HPC, TC, FC, and FS) for 7 months in Sooc Bridge and Carpenter’s Bridge using bottom sediments for samples. Sediment was used because bacteria accumulate over time in the sediment due to high amounts of organic and inorganic matter in the substrate and this leads to better survivability of bacteria. The values obtained from microbiological parameters investigated are generally higher in Sooc Bridge. HPC results show that the higher mean value was in Sooc Bridge(7.43 x 108 CFU/lOOg). The levels of TC on both sampling sites ranged from 9.5 x 102to 2.40 x 104 CFU/100g. For TC, Sooc has the higher mean (1.76 x 104 CFU/lOOg) for 7 months. FC values ranged from 3.0 x 101 to 2.4 x 104 CFU/lOOg. Sooc bridge has the higher mean(1.17 x 104 CFU/lOOg) between the 2 sites. Lastly, the FS value range from 2.9 x 103 to 2.4 x 104 CFU/lOOg. Also, Sooc Bridge has the higher mean value (1.58 x 104 CFU/lOOg). There are no significant differences on the microbiological parameters used in each site. The FC/FS ratio revealed that mixed fecal materials contaminate the river. Among the four pathogenic bacteria detected, Salmonella spp. was the most abundant for 7 months (63-65%) and E. coli was the least abundant(>l%). Based on the criteria on sediment quality evaluation, there is little to moderate loading of organic and inorganic matter.