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    Development of probiotics determination of LD50 of previously isolated indigenous vibriolytic bacteria, Pseudomonas aerugginosa JP4, to Penaeus monodon postlarvae
    Billena, Ma. Thesa M. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2000-06)
    Previous studies showed that Pseudomonas aeruginosa JP4 is effective in eliminating pathogenic Vibrio spp. on shrimp. However, virulence studies on this strain are lacking. Hence, this study was conducted to test the pathogenicity of P. aeruginosa JP4, using different bacterial densities of 105, 106, 107, 108, 109 and 1010 CFU/ml. These were introduced to Penaeus monodon postlarvae 13 (PL13)and early juvenile (PL25) stages. After 3 days of monitoring, mortality rates and LD50 values were determined using Reed-Muench method. Results showed that shrimps can not tolerate higher bacterial densities with LD50 of 1.15 x 107 CFU/ml and 1.12 x 108 CFU/ml for PL13 and PL25 respectively. The relatively high LD50 values on shrimp stages PL13 and PL25 indicate a low virulence of the bacterial strain. Hence, P. aeruginosa JP4 maybe used as potential vibriolytic bacteria.
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    Determination of the fungal flora in the rearing water, eggs, larvae and post-larvae of Penaeus monodon in a large-scale hatchery system
    Baes, Glanee F. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1995-03)
    The rearing water and homogenized. eggs, larvae, post-larvae of Penaeus monodon were taken front the large-scale hatchery system. These were counted, isolated and identified. Analysis was done by determining the mean, standard deviation and skewness of the data. The result showed that the skewness of the yeast and mold counts in the rearing water were 0.93 and 0.64, respectively 'while that in the homogenized samples were 0.92 and 1.09, respectively. These showed that the data were positively skewed, hence, fungi were greater in the early larval stages than in the later stages. The mean showed that yeast yielded greater counts as compared to the molds. Furthermore, rearing water have also higher fungal count than that of the homogenized prawns. The fungi identified were Aspergillus, Balanium, Bispora, Cladosporium and Phialomysis. In the study, the fungi identified were the normal mycoflora which did not adversely harm the prawn due to favorable hatchery conditions, there being no disease outbreak during the duration of the study.
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    Treatment of Vibrio harveyi-infected Penaeus monodon using Alibutbut (Tabernaemontana pandacaqui Poir.) plant powder
    Bacaling, Marivic T. (Division of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Visayas, 2005-04)
    The cytotoxicity test of crude methanolic leaf extract using Brine Shrimp Lethality Assay revealed that Alibutbut (Tabernaemontana pandacaqui Poir.) is safe to use in treating vibrio-infected penaeid shrimps. The effectiveness of the alibutbut powder against vibriosis was examined through the determination of the post-larvae 20 (PL 20) penaeid shrimp mortality and the water microbial assay after 48 hours. Percent penaied shrimp mortality (0-6.67%) and microbial count (2.91 x 10^4- 3.26 x 104 cfu/mL) of the plant treatments were significantly lower compared to the negative control (no treatment) that exhibited 50% penaeid shrimp mortality and a microbial count greater than 6.00 x 10^4 cfu/mL. The results of the experiment showed that Alibutbut plant powder is indeed effective in controlling Vibrio harveyi bacteria.
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    Effect of Physico-chemical parameters of rearing waters on survival rates of larvae and post larvae of Penaeus monodon in two hatchery systems
    Alindong, Ray Lauron (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 1995-03)
    Physico-chemical parameters such as temperature, pH, salinity, and D.O. of the small-scale and large-scale hatchery system were monitored daily for a duration of two months. The relationship between the physico-chemical parameters and the survival of the different larval and post-larval stages between the two hatchery systems was then determined. Results showed that the physico-chemical parameters obtained from the small-scale hatchery system were favorable to the survival of the larval stages of the prawn. However, the physico—chemical parameters obtained from the large-scale hatchery system were more suitable for the growth of the post-larval stages.