UPV Theses and Dissertations
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Item Fungal pathogen associated with Rhizophora stylosa: A comparative study between oiled and unoiled sites within Taklong Island National Marine Reserve (TINMAR), Nueva Valencia, GuimarasBalanga-an, Kristoffer (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2009-03)Mangroves, refer to an assemblage of trees and shrubs that inhabit the coastal intertidal zone in tropical and subtropical regions. They help in the soil formation, protection, and stabilization of our coastal environment as well as serve as shelter and breeding grounds for some of our marine organisms. A mangrove habitat represents the borderline between land and sea and is considered as one of the principal places where spilled oil and associated impacts converge. Oil spill threaten mangroves by predisposing the plants to fungal attacks thereby reducing their resistance to stress and diseases. Studies also show that certain fungi species are able to utilize oil in contaminated mangrove species. This study generally aims to compare the fungal population found on the diseased leaves of Rhizophora stylosa between oiled and unoiled sites in the Taklong Island National Marine Reserve (TINMAR). It also aims to isolate, characterize and identify fungi present in diseased leaves of Rhizophora stylosa using potato dextrose agar (PDA) culture media and determine their frequency of occurrence. Leaf samples were collected from two sites in TINMAR, one of which was contaminated by oil and the other one was not. Three trees were selected to represent each of the two sampling sites (oiled and unoiled), and to each of the selected trees, three diseased leaves were collected. The results of this study showed the dominance of some species of fungi namely, Aspergillus (40%), Penicillium (20%), Cladosporium (13.33%), Monilia (6.67%), Mycelia (6.67%), Humicola (6.67%), and Pestalotiopsis (6.67%). Five out of these seven genera were isolated from the unoiled site while six of these were isolated from the oiled site. Generally, the fungal population was observed to be greater in the oiled site than in the unoiled. This preliminary data indicate that R. stylosa might still be undergoing stress due to the remaining components of the spilled oil in the area. However, this can only be resolved by further monitoring.Item Community structure of mangroves in selected barangays in Sebaste, AntiqueAlorro, Mary Juandin Grace F. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2007-03)Mangroves are higher plants that are primarily found predominantly in the intertidal areas such as estuaries and lagoons of the tropical and subtropical shorelines. This study was conducted to assess the community structure of mangroves in Barangay Callan, Barangay Idio, Barangay Poblacion, Barangay Abiera, and Barangay Bacalan, Sebaste, Antique, using the transect line method. Specifically, it aimed to determine selected physical and chemical factors such as salinity, pH, temperature for water and soil as well as characterization of the substrate; identify and classify true mangrove species present in the area; and describe the community structure in terms of stand basal area, stems per hectare, relative density, relative frequency, relative dominance, importance value, Shannon index of diversity, zonation patterns, seedling and sapling density. No study has been done to document the community structure of mangroves in Sebaste, Antique thus, this study was done to provide vital information needed for its proper management. Three stations were selected in Sebaste, Antique. Four (4) species of true mangroves were identified, belonging to three (3) families: Palmae - Nypa fruticans; Rhizophoraceae - Rhizophora apiculata, Bruguiera gymnorrhiza; and Euphorbiaceae - Excoecaria agallocha. The study area had a water salinity range of 0.6 ppt to 15.3 ppt; water temperature range of 28.3°C to 35.4°C; water pH range of 7.1 to 7.7; soil temperature range of 28.3°C to 34.7°C; and soil pH range of 6.5 to 7.2. Basal area ranges from 3.16 m2/ha (Rhizophora apiculata) to 5.88 m2/ha (Excoecaria agallochd) to 8.19 m2/ha (Bruguiera gymnorrhiza) . The species with the highest average of stems per hectare were Excoecaria agallocha and Bruguiera gymnorrhiza with 300 as well as the highest average density value of 100 for Excoecaria agallocha. Nypa fruticans had the highest relative frequency and relative dominance. Among the four species, the most important species was Nypa fruticans. The Shannon Index of Dominance was 0.403 which was quite low in terms of diversity as influenced by the dominance of Nypa fruticans.Item Species composition and distribution of mangrove soil fungi from Iloilo River, Iloilo City, PhilippinesAlmoros, Peter Paul C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2018-06)Fungi are very diverse and ubiquitous microorganisms. Fungal diversity is an important aspect of the ecosystem that needs to be studied. There have been many studies regarding biodiversity of fungi in terrestrial, marine and riverine environments, but studies regarding fungi found in mangrove soil still needs to be done. This study focused on determining the species composition, distribution and ecology of mangrove soil fungi in the Iloilo River, Iloilo City. Soil fungi were sampled from three areas in the river representing the downstream, midstream and upstream areas. Fungal isolates were identified based on morphological characteristics- cultural and microscopic features. There were 10 identified fungal species -Aspergillus cf. flavus, Aspergillus cf. niger, Aspergillus cf. ustus, Aspergillus cf. versicolor, Aspergillus sp. (1), Aspergillus sp. (2) and Aspergillus sp. (3), Geotrichum cf. candidum, Penicillium cf. citrinum and Penicillium sp. (1), while there were 2 unidentified species. Three of these species were very frequent across the sites -Aspergillus cf. flavus, Geotrichum cf. candidum and Penicillium cf. citrinum.Item Short-term impacts of oil spills on fungal population in a mangrove area in Batad, IloiloAlpas, Donna Bella C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)Oil spills cause extensive damage on the marine environment, ranging from obliteration of its flora and fauna to biomagnifications of toxic components of petroleum. The location of mangroves at the sea-land interface makes the ecosystem susceptible to disturbances such as oil spills. There is a considerable information on the impacts of oil spills on mangrove while impacts on microbial populations, especially fungi, are limited. This study therefore assessed the effect of oil on the diversity and distribution of fungi of oil-contaminated sites in Batad, Iloilo due to Power Barge oil spill last November 8, 2013. The mangrove surface sediments were collected on January 11, 2014 at oil-contaminated sites in Batad, Iloilo and uncontaminated site in Ajuy, Iloilo. Sample were processed based on Pepper and Gerba (2004). Colony forming units per gram of soil (CFU/g) was used to express yeasts and filamentous fungi. On PDA medium, more fungal isolates were isolated at oil-contaminated site compared with uncontaminated site at 14 and 12 species respectively, there was also a higher Shannon Index of Diversity at 2.4858 and 2.4582, respectively. Among the filamentous fungi, three genera were represented by Aspergillus. Penicillium, and Cladosporium. However, Aspergillus was more diverse with 10 species. Six species were found only at the oil-contaminated sites - Aspergillus sp 2. Aspergillus sp 1, Cladosporium cladosporioides, Penicillium sp 2, Penicillium sp 3 and Yeast sp 2 while four species are found in the unoiled site - Penicillium cf. lapidosum, Aspergillus sp 3, Aspergillus sp 4 Penicillium sp 1. Furthermore, there is a low index of similarity as shown by Jaccards coefficient of similarity (J=0.44) and Sorensen coefficient of similarity ( C=0.61). Fungal load was higher at oil-contaminated soils compared with uncontaminated soils at 1.3 x 10 - CFU/g and 6.0 x IO3 CFU/g, respectively. Bushnell Haas Agar yielded more fungi isolates isolated from oil-contaminated sites compared with uncontaminated site of 4 and 3 species respectively. Furthermore, a higher Shannon Index Diversity (H) was observed at oil-contaminated sites (1.3298) than those at uncontaminated site (0.6364) suggesting that there are more species of fungi capable to degrade the hydrocarbons and utilize it as a source of energy. The overall frequency of distribution of the major fungal genera in both oil-contaminated and uncontaminated sites showed that only Dueteromycota was isolated and dominated in both sites and in both media, the PDA and Bushnell Haas Agar. The major genera isolated in PDA medium included Aspergillus, Penicillium, Cladosporium, and yeast. In Bushnell Haas Agar, Aspergillus and yeast were the major genera isolated and are seen in both sites. The frequency of occurrence of various species in uncontaminated and oil-contaminated sites were not significantly different. The Deuteromycota were the dominant group that were isolated on all sites using two media. The overall result indicate the stimulating effect of oil are observed in the higher fungal load in oil- contaminated site.Item Living above and belowground biomass and carbon stock assessment of station 1, Taklong Island, Taklong Island National Marine Reserve, Guimaras, PhilippinesAlegado, Alexis Jude T. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2018-06)The Taklong National Marine Reserve is one of the marine protected sites in the country with a good cover of mangroves. This study assessed the species composition, diversity, aboveground biomass and the estimated carbon of the natural mangrove forest found in Taklong Island Station 1. Barangay Lapaz. Nueva Valencia. Guimaras. The study aimed to: a.) map out the area of the selected mangrove patches, b.) identify the species composition and diversity of the sampling site, c.) quantify the aboveground and belowground biomass, carbon stock of live vegetations, and d.) to convert the carbon stock to carbon dioxide equivalence (CO₂e) of the selected station. This study is only limited to the estimation of living aboveground, belowground and the carbon stock using the published allometric equations. Only 7 species of mangroves were found in the site belonging to 3 families. The mean stand basal area was 21.47 m2 ha-1. The species diversity is low at 0.23 due to low speciation of species of mangroves on the site. The total aboveground biomass was 506.89 Mg ha-1 and carbon dioxide emission potential (CO₂e) of 2,498.15Mg CO₂e. This shows that mangrove in Station 1 of Taklong Island has stored a considerable amount of carbon overtime. Hence, there must be a proper management and protection of this ecosystem.Item Fungal pathogens associated with Sonneratia alba: A comparative study between oiled and unoiled sites within Taklong Island National Marine Reserve (TINMAR), Nueva Valencia, GuimarasAlbacete, May Therese F. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2009-03)Majority of researches on impacts of oil spills on mangroves focused on the immediate post-spill acute effects. However, the persistence of oil in sediments at oil spill sites has been reported in a number of published works. As such, this remaining oil could serve as source of stress to mangroves resulting into the expression of sub-lethal effects such as changes in foliage and canopy, abnormalities in reproductive structures among others. Furthermore, oil in mangroves also has the potential of increasing fungal attacks due to their impairment of natural defense mechanisms such as salt removal from leaves and trunks. Thus, this study was conducted to determine if there was a difference in fungal species composition and frequency of occurrence of fungal population of mangrove leaves collected in oiled and unoiled areas within Taklong Island National Marine Reserve, Nueva Valencia, Guimaras following the spill that occurred in August I1,2006. Fungi from diseased leaves of Sonneratia alba were isolated and characterized using Acidified Potato Dextrose Agar (APDA) for samples collected in January 2009 only. A total of 7 and 6 species were identified from among the isolates in oiled and unoiled sites. Although there is a little difference in species composition, almost all the isolated species was frequently occurring in both oiled and uncoiled sites. Gliomastix morurom (5.8%) in the unoiled site was only frequently occurring. The results may imply that difference in frequency of occurrence of certain species might be an indicator for sub-lethal effects of oil but needs further investigations to consider environmental factors among other factors.Item Community structure, biomass production and carbon stock assessment of mangroves in Barangay Ambolong, Batan, AklanAgregado, Leizel B. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2013-04)Mangroves are evergreen shrubs and trees of several unrelated families that thrive within intertidal zones of sheltered flats, coves, bays, and estuarine regions extending inland along streams and river where water is primarily brackish. Continued threats to their existence call for their protection and conservation. This study was conducted to assess the community structure of mangroves in Brgy. Ambolong, Batan, Aklan by 1). measuring physico-chemical parameters (salinity, temperature, pH) 2). describing the community structure in terms of and in terms of a) basal area, b) stems per hectare, c) relative density, d) relative frequency, e) relative dominance, f) importance value, g) Index of Diversity, h) seedling and sapling density, and i) zonation pattern; 3). to assess the bidmass production and carbon stock. This study was done to provide preliminary information regarding the current status of mangroves in the area. Three (3) transect lines, each containing three (3) plots, were established in the area. Twenty (20) mangrove species belonging to eleven (11) families were identified: Acanthaceae - Acanthus ebreatus, Acanthus volubilis; Arecaceae - Nypa fruticans; Avicenniaceae - Avicennia alba, Avicennia marina, Avicennia officinalis, Avicennia rumphiana; Bombaceae — Camptostemon philippinensis; Euphorbiaceae - Excoecaria agallocha; Meliaceae — Xylocarpus grioanatum, Xylocarpus moluccensis; Myrsinaceae — Aegiceras corniculatum; Myrtaceae - Osbornia octodonta; Rhizophoraceae - Bruguiera cylindrica, Ceriops decandra, Rhizophora apiculata, Rhizophora mucronata, Rhizophora stylosa; Rubiaceae - Scyphiphora hydrophyllacea; Sonneratiaceae - Sonneratia alba. Water salinity in the study area ranged from 27-30 ppt; soil temperature ranged from 28°C to 30°C. Avicennia marina had the highest values for basal area (4.22 m2/ha), stems per hectare (15,700 stems per hectare), relative density (63.56), relative frequency (63.56%), relative dominance (48.10%), and was the most important species with the importance value of 175. Rhizophora stylosa had the lowest values for basal area (0.002 m2/ha), stems per hectare (100 stems/ha), relative density (0.40 trees/ha), relative frequency (0.4%), relative dominance (0.23%), and was the least important species, having the importance value of 0.832. Rhizophora apiculata and Avicennia officinalis, together with Rhizophora stylosa, had the lowest value for stems per hectare (100 stems/ha), relative density (0.40 trees/ha), and relative frequency (0.4%). Seedling density was highest for Avicennia alba(187 seedlings/ha) and lowest for Bruguiera cylindrica (1 seedling/ha). Sapling density was highest for Avicennia marina (54 saplings/ha) and lowest for Excoecaria agallocha (1 sapling/ha). The Index of Diversity was H=0.598. The highest recorded litter was 336.6 g in December 19, 2012-January 2, 2013 while the lowest was recorded at 18.2 grams in December 5-December 29, 2012. Highest biomass production was that of Avicennia marina (962.94 tons/ha), while the lowest was Avicennia officinalis (0.009 tons/ha). Avicennia marina had the highest carbon stock value of 433.32 tons/ha; the lowest was that of Avicennia officinalis (0.004 tons/ha).Item Density of mangrove trees in selected municipalities in the Province of Antique, Panay Island, PhilippinesArelis, Ian Jerwin C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2006-03)Mangroves line one quarter of the world’s tropical coastlines, and approximately 117 countries and territories have mangrove resources within their borders. Although over recent years mangrove deforestation has occurred at a phenomenal rate worldwide, constant assessment in the Philippines has been made to monitor its current status. To characterize the density of mangrove trees better in Panay Island, surveys were undertaken within the coastal and riverine estuaries in the 11 municipalities of the province of Antique. Detailed records were taken by transect and ocular surveys along 40 barangays with 75 transects and 223 plots established. The mangroves of Antique were composed of 31 species of true mangroves belonging to 14 families and a total of 16 generas. Considerable values in terms of relative density and stems per hectare were found with Nypa fruticans as the highest, with a relative density of 70.372% trees per hectare and an average stem density of 4,457.249 stems/ha. Other significant species included Sonneratia alba (4.534%; 625 stems/ha), Avicennia marina (4.524%; 1,525 stems/ha), and Rhizophora apiculata (4.232%; 50 stems/ha). Close examination of the data suggested the variability of distribution patterns and density to be attributable to the differing responses of individual species to the environmental factors and other human activities. It was also concluded that habitat classification and topography could directly affect mangrove distribution and also capable of altering basic zonation patterns.Item Physiochemical factors affecting mangrove distribution and occurrence in selected sites in the Province of AntiqueAgapin, Antonio, Jr. D. (Division of Biological Sciences, Bachelor of Science in Biology, University of the Philippines Visayas, 2009-03)Mangrove forests are unique inter-tidal ecosystems comprised of taxonomically diverse, salt-tolerant tree and other plant species that are primarily found o.i relatively sheltered coastlines, deltas, estuaries, and lagoons in tropical and subtropical regions of the world. The objective of this study is to generally determine the physicochemical factors influencing the distribution and occurrence of mangroves in selected sites in the Province of Antique and to specifically measure selected physicochemical factors influencing the distribution and occurrence per species per municipality and correlate selected physicochemical factors like soil and water salinity, soil and water pH, and soil and water temperature influencing the distribution and occurrence per species per municipality using Pearson Correlation Coefficient. There were 224 plots established in 11 municipalities in the province of Antique. Twenty-five plots were established in Anini-y, 44 in Hamtic, 11 in San Jose, 5 in Belison, 18 in Patnongon, 23 in Bugasong, 11 in Barbaza, 9 in Tibiao, 26 in Culasi, 23 in Pandan, and 29 in Semirara. A total of 27 true mangrove species belonging to 12 families were found in the 11 municipalities of Antique. The 12 families are Family Avicenniaceae represented by Avicennia alba , Avicennia marina, Avicennia officinales, and Avicennia rumphiana', Family Combretaceae represented by Lumnitzera racemosa and Lumnitzera littorea; Family Euphorbiaceae represented by Excoecaria agallocha; Family Lythraceae represented by Pemphis acidula; Family Meliaceae represented by Xylocarpus granatum and Xylocarpus moluccensis; Family Myrsinaceae represented by Aegiceras coniculatum and Aegiceras floridum; Family Myrtaceae represented by Osbornia octodonta; Family Arecaeae (Palmae) represented by Nypa fruticans; Family Rhizophoraceae represented by Bruguiera cylindrica, Bruguiera gymnorrhiza, Bruguiera sexangula, Ceriops decandra, Ceriops tagal, Rhizophora apiculata, Rhizophora mucronata, and Rhizophora stylosa; Family Rubiaceae represented by Scyphiphora hydrophyllacea; Family Sonneratiaceae presented by Sonneratia alba, Sonneratia caseolaris, Sonneratia ovata; and Family Sterculiaceae represented by Heritiera littoralis. For Soil pH, the highest value of 8.87 was observed in the municipality of Hamtic and the lowest at 6.32 in the municipality of Semirara. Overall mean soil pH was 7.31. For Water pH, the highest value of 8.96 was observed in the municipality of San Jose, and the lowest value of 6.29 in the municipality of Tibiao. Overall mean water pH was 7.23. For salinity, it ranged from 3.1 ppt (Tibiao) to 27.47 (Semirara) and the overall mean was 13.37 ppt For Water Temperature, the highest value of 29.48 °C was observed in Anini-y & the lowest value of 24.74 °C in the municipality of Semirara with an overall mean of 27.40 °C. For Soil Temperature, the highest value of 30.2 °C was observed in the municipality of Belison & the lowest value of 24.97 °C in the municipality of Semirara with an overall mean of 27.50 °C. Among the species present, Aegiceras floridum showed significant correlation with Soil pH (favor low soil pH), water pH ( favor low water pH), salinity ( favor high salinity), soil temperature (favor high soil temperature) and water temperature (low water temperature), A. rumphiana showed significant correlation with water pH (favor low water pH) and soil temperature ( favor low soil temperature), B. cylindrica showed significant correlation with soil pH ( favor high soil pH), C. decandra showed significant correlation with water pH (favor low water pH) and salinity (favor high salinity), L. littorea showed significant correlation with soil pH (favor high soil pH), water pH (favor high water pH), salinity (favor high salinity), soil temperature (favor high soil temperature) and water temperature (favor high water temperature), O. octodonta showed significant correlation with soil pH (favor high soil pH), water pH (favor low water pH), salinity ( favor low salinity), soil temperature (favor high soil temperature) and water temperature (favor low water temperature), S. caseolaris showed significant correlation with water pH (favor low water pH), salinity (favor low salinity), soil temperature (favor low soil temperature) and water temperature (favor low water temperature) and S. ovata showed significant correlation with soil pH ( low soil pH), salinity (favor low salinity) and water temperature (favor low water temperature). Species like A. corniculatum, A. alba, A. marina, B. sexxangula, E agallocha, H. littoralis, L racemosa, N. fruticans, R apiculata, R mucronata, R stylosa, S. alba, X. granatum and X. moluccencis showed wide tolerance (no correlation) in all physicochemical factors (Soil Salinity, Water pH, Soil pH, Soil Temperature and Water Temperature).