UPV Digital RepositoryUPV-DRUniversity of the Philippines Visayas
 

Undergraduate Research Paper

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14583/28

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Degree: search.filters.degree.Bachelor of Science in Biology

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    Analysis and assessment of the performance of the Municipal Leadership and Governance Program (MLGP) partner municipalities in improving health outcomes
    Araneta, Francine Nicole M.; Bebita, Valerie Anne N.; Endoma, Vivienne F.; Estanda, Marvyn Kerziel A.; Gupeteo, Mary Pauline Kriszl E.; Lebosada , Billy John F.; Magsipoc, Karla Mae B.; Pabello, Kimberly T.; Samoro, Karmela Marie G.; Villaruel, Rigel Kent Q. (2016-05)
    The Zuellig Family Foundation through the Municipal Leadership and Governance Program (MLGP) aims to bridge the gap between the Filipinos and the promise of universal health care through the local health managers. They developed a local health scheme with focus on the World Health Organization’s six building blocks of health system so the country can achieve the Millennium Development Goals on health by 2015. However, there are still no available methods that can measure the success of health programs such as the MLGP. In order to address this issue, the researchers proposed to quantify and assess the health outcomes and the municipality performance. Heath outcomes are represented using secondary data provided by the MLGP municipalities on the performance indicators for MDGs for health. Municipality performance is quantified using a scoring system developed by the researchers based on the performance indicators assigned by the ZFF for each WHO building-block and significant improvement in municipality performance was found. The health data was analyzed by comparing the medians of yearly data using non- parametric/distribution-free sign tests. Among the health indicators with complete data for all 28 municipalities, significant difference in health outcomes was found in the data proportions of ratio of facility-based deliveries (FBD), ratio of births attended by births attended by skilled personnel (SBA), contraceptive prevalence rate (CPR), and proportion of fully immunized children (FIC). Maternal and infant mortality ratio, under-five mortality rates and malnutrition rate did not show any significant difference within the two-year intervention time span. Ratio of facility-based deliveries and ratio of births attended by skilled personnel showed significant positive correlations with health financing, health service delivery, and total performance indicators. There is also a significant positive correlation between CPR and leadership and governance, health financing, health human resource, access to medicine and technology, health service delivery and total performance indicators. However, it is interesting to note that the data on fully immunized children showed significant but negative correlations with leadership and governance performance indicators.
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    Dengue knowledge, policies, and Aedes Larval population in selected low and high dengue priority areas in Miagao, Iloilo
    Amihan, April Rose D.; Basco, Arriane Mae P.; Escosia, Edmon B.; Monsale, April Joy P.; Solis, Jann Christian V.; Varon, Marianne Joy C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2009-03)
    This cross-sectional study aimed to compare the knowledge, practices, and Aedes larval populations between low and high dengue priority areas of Miagao, Iloilo. Questionnaire-guided interviews were conducted to assess the knowledge and practices of the study population. Larval surveys were done to determine Aedes larval populations, expressed as House Index (HI), Container Index (CI), and Breteau Index (BI). Results showed that respondents from the low priority area were more knowledgeable on dengue than the respondents from the high priority area (p<0.05). Dengue preventive practices were found to be the same for both low and high priority areas. There was, however, no significant relationship between dengue knowledge and dengue preventive measures (p<0.05). Results from (he larval survey revealed that both low and high priority areas have HI and BI above the WHO standards (HI<5%; BI<20) and are considered “high risk” areas. Lastly, age (p<0.01) and educational attainment (p<0.05) were found to affect an individual’s dengue knowledge.
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    Antibiotic resistance patterns of selected pathogenic bacteria isolated from water and bottom sediments in the Iloilo tidal inlet (Iloilo River)
    Agustin, Eda Mae S.; Cabrias, Ligaya April May S.; Fuentebella, Elaine Joyce V.; Gestuveo, Rommel J.; Lagon, Raymond Anthonn Kriztee J.; Saldajeno, Cypruz Anne L.; Tunguia, Saer Martin J.; Villanueva, Regene Patricia Natividad V. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2010-03)
    In recent years, antibiotic resistance of pathogenic bacteria has become a public health concern. Although little is known of its environmental implications the presence of both potentially pathogenic bacteria and fecal coliform in rivers and other bodies of water raises the question of whether resistance transfer may actually occur in bodies of water. In relation, the Iloilo River for one has experienced changes in both ecological and sanitary conditions. With this threatening problem of both ecological and fecal pollution, the river could harbor microflora that could be of public health concern. Using the Kirby-Bauer Method, this study generally aims to determine the antibiotic resistance of pathogenic bacteria isolates (E. coli, S. typhi and V. cholerae) from water and bottom sediment samples from selected sampling areas in Iloilo River, Iloilo City. Stock cultures of bacterial isolates were obtained from the UPV-National Institute of Molecular Biology and Biotechnology (UPV-NIMBB) and were subjected to antibiotic resistance analysis using 12 different antibiotic classes. Results showed varying degrees of resistance in all of the sampling sites and species. Most isolates were highly resistant to penicillin and oxacillin and least resistant to ciprofloxacin. Multidrug-resistant isolates were also observed in all of the sampling sites and species. More isolates from bottom sediments were observed to be highly resistant than the isolates from water samples. Pearson correlation revealed that the antibiotic resistance patterns among the different species isolated from the different sources and sampling sites appeared to vary in a systematic manner indicative of a similar mechanism of antibiotic resistance development.
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    Identification and antibiotic resistance of enterococcus (Schleifer and Kilpper-Bälz 1984) species isolated from Iloilo river
    Ansino, Jan Reygine G. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2014-04)
    The presence of Enterococci species in the water has long been regarded as an indicator of fecal contamination. With the increasing rate of antimicrobial resistance globally, infections caused by enterococcal species became difficult to treat. The study determined the species distributions and antimicrobial resistance profiles of Enterococcus species isolated from water and sediment samples in the Iloilo River. Susceptibility of the isolates was tested against ampicillin, penicillin, ciprofloxacin, chloramphenicol, doxycycline, erythromycin, tetracycline, and vancomycin. Among the 115 fecal coliform samples, a total of 61 (53%) isolates were confirmed to belong to the genus Enterococcus. Of these, 40% were identified as Enterococcus faecalis, followed by Enterococcus faecium 33% and Enterococcus avium (4%). Isolates showed resistance to tetracycline (51%), erythromycin (18%), and doxycycline (13%) and a high level susceptibility to ampicillin (100%), chloramphenicol (93%), and penicillin (90%). Multiple antibiotic resistance patterns revealed resistance to at least four antibiotics. Six (12%) vancomycin-resistant Enterococci (VRE) were detected. The results revealed low level of antibiotic resistance in the river. However, the presence of these fecal contaminants in the river can still pose a potential risk to the human health and the of spread of antibiotic resistance genes, indicating the great importance of improving risk management practices in the river.
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    Biosorption of Copper (CU) and Lead (PB) by heavy metal-resistant bacterial isolates from Iloilo River
    Ampuan, Naira Mitz W. (Division of Biological Sciences, College of Arts and Sciences,University of the Philippines Visayas, 2012-04)
    Many indigenous organisms isolated from sites contaminated with heavy metals have tolerance to heavy metal toxicity and these microbial activities have always been the natural starting point for all biotechnological applications. It is therefore necessary to isolate bacterial strains with novel metabolic capabilities. In this study, a total number of 18 heavy metal-resistant bacteria (10 Pb-resistant and 8 Cu-resistant) were isolated from Iloilo River sediment samples (10° 41.974’N, 122°34.187’ E). Biosorption of Cu and Pb by the bacterial isolates was characterized to evaluate their applicability for heavy metal removal from industrial waters. Selecting the highest resistance to metal toxicity’ and biosorption, 4 isolates were identified by phenotypic characterization. Identification of the top four metal-resistant isolates designates: Vibrio charchariae (83%) for Cu4; Vibrio harveyi (79%) for Cu6; Moraxella equi (100%) for Pb8; and Phoecoenobacter uteri (92%) for Pb9. Evaluation of specific metal biosorption by the isolates after incubation for 48 h suggested that none of the isolates have good potential for bioremoval of metals from contaminated environments.
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    Antibacterial activity of Aqueous and Methanol extracts of selected Basidiomycetes from UPV, Miagao, Iloilo, Philippines
    Ambubuyog, Marja Victoria U. (Division of Biological Science, College of Arts and Sciences, University of the Philippines, 2013-03)
    The Basidiomycetes represent an enormous source for natural products with diverse chemical structures and wide-ranging antimicrobial activity. This study was done to investigate the antibacterial property of selected species of basidiomycetes aqueous and methanolic extracts against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Specifically, it aimed to (1) determine the antibacterial activity of the aqueous extract of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa based on the zone of inhibition; (2) determine the antibacterial activity of the methanolic extract of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa based on the zone of inhibition; and (3) compare the aqueous and methanolic extracts of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa based on the zone of inhibition. A total number of ten species were collected namely Auricularia sp., Schizophyllum sp., Trametes pubescens, Trametes versicolor, Trametes elegans, Ganoderma sp.(l), Ganoderma sp.(2), Stereum sp. and two unknowns. Dried samples were crushed and extracted with water and methanol. Antibacterial activity was determined using the Kirby-Bauer disc diffusion method. The results showed a negative to weak inhibition against the test microorganisms which is inconsistent with the results of previous studies. It could possibly be attributed to the preparation of the samples and the fruiting body maturity that influenced the antibacterial potential of the samples. It appears that fruiting bodies used in the present study may not be reliable sources of bioactive compounds for antibacterial activity.