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Undergraduate Research Paper

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14583/28

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Degree: search.filters.degree.Bachelor of Science in BiologySDG: search.filters.sdg.SDG 15 - Life on land

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    Dengue knowledge, policies, and Aedes Larval population in selected low and high dengue priority areas in Miagao, Iloilo
    Amihan, April Rose D.; Basco, Arriane Mae P.; Escosia, Edmon B.; Monsale, April Joy P.; Solis, Jann Christian V.; Varon, Marianne Joy C. (Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 2009-03)
    This cross-sectional study aimed to compare the knowledge, practices, and Aedes larval populations between low and high dengue priority areas of Miagao, Iloilo. Questionnaire-guided interviews were conducted to assess the knowledge and practices of the study population. Larval surveys were done to determine Aedes larval populations, expressed as House Index (HI), Container Index (CI), and Breteau Index (BI). Results showed that respondents from the low priority area were more knowledgeable on dengue than the respondents from the high priority area (p<0.05). Dengue preventive practices were found to be the same for both low and high priority areas. There was, however, no significant relationship between dengue knowledge and dengue preventive measures (p<0.05). Results from (he larval survey revealed that both low and high priority areas have HI and BI above the WHO standards (HI<5%; BI<20) and are considered “high risk” areas. Lastly, age (p<0.01) and educational attainment (p<0.05) were found to affect an individual’s dengue knowledge.
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    Biosorption of Copper (CU) and Lead (PB) by heavy metal-resistant bacterial isolates from Iloilo River
    Ampuan, Naira Mitz W. (Division of Biological Sciences, College of Arts and Sciences,University of the Philippines Visayas, 2012-04)
    Many indigenous organisms isolated from sites contaminated with heavy metals have tolerance to heavy metal toxicity and these microbial activities have always been the natural starting point for all biotechnological applications. It is therefore necessary to isolate bacterial strains with novel metabolic capabilities. In this study, a total number of 18 heavy metal-resistant bacteria (10 Pb-resistant and 8 Cu-resistant) were isolated from Iloilo River sediment samples (10° 41.974’N, 122°34.187’ E). Biosorption of Cu and Pb by the bacterial isolates was characterized to evaluate their applicability for heavy metal removal from industrial waters. Selecting the highest resistance to metal toxicity’ and biosorption, 4 isolates were identified by phenotypic characterization. Identification of the top four metal-resistant isolates designates: Vibrio charchariae (83%) for Cu4; Vibrio harveyi (79%) for Cu6; Moraxella equi (100%) for Pb8; and Phoecoenobacter uteri (92%) for Pb9. Evaluation of specific metal biosorption by the isolates after incubation for 48 h suggested that none of the isolates have good potential for bioremoval of metals from contaminated environments.
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    Antibacterial activity of Aqueous and Methanol extracts of selected Basidiomycetes from UPV, Miagao, Iloilo, Philippines
    Ambubuyog, Marja Victoria U. (Division of Biological Science, College of Arts and Sciences, University of the Philippines, 2013-03)
    The Basidiomycetes represent an enormous source for natural products with diverse chemical structures and wide-ranging antimicrobial activity. This study was done to investigate the antibacterial property of selected species of basidiomycetes aqueous and methanolic extracts against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Specifically, it aimed to (1) determine the antibacterial activity of the aqueous extract of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa based on the zone of inhibition; (2) determine the antibacterial activity of the methanolic extract of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa based on the zone of inhibition; and (3) compare the aqueous and methanolic extracts of selected species of basidiomycetes in inhibiting the growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa based on the zone of inhibition. A total number of ten species were collected namely Auricularia sp., Schizophyllum sp., Trametes pubescens, Trametes versicolor, Trametes elegans, Ganoderma sp.(l), Ganoderma sp.(2), Stereum sp. and two unknowns. Dried samples were crushed and extracted with water and methanol. Antibacterial activity was determined using the Kirby-Bauer disc diffusion method. The results showed a negative to weak inhibition against the test microorganisms which is inconsistent with the results of previous studies. It could possibly be attributed to the preparation of the samples and the fruiting body maturity that influenced the antibacterial potential of the samples. It appears that fruiting bodies used in the present study may not be reliable sources of bioactive compounds for antibacterial activity.